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Химический состав можжевельника сибирского каллусной ткани и послеэкстракционного остатка.

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. 2012.
2. . 145–150.
581.192:582.477.6
,
©
*
.
,
.
,
.
,
(
.
82,
), e-mail: shjlv@mail.ru
,
.
–
Juniperus sibirica
.
Burgsd.
.
:
,
,
,
,
,
-
.
Juniperus sibirica Burgsd. –
.
,
,
,
,
,
-
[1].
–
,
[1–3],
,
-
.
[4].
.
,
,
-
,
[2, 5].
,
,
-
[6].
.
,
-
[7].
.
,
–
,
-
[3, 7].
,
-
[7],
(
)
e-mail: shjlv@mail.ru
–
.
,
.
,
,
–
, e-mail: dk@xf.tsu.ru
,
,
[8],
,
–
, e-mail: enaesh@mail.ru
,
*
[8].
,
,
.
-
146
.
,
[8].
.
,
.
Juniperus sibirica,
0,8–0,9% [9].
,
2,11% [8].
,
:
,
,
,
,
80%
,
Juniperus sibirica,
,
[8].
,
,
[10].
,
-,
,
.
,
, -,
,
,
2% 3-
),
8%
,
,
78,9% [8].
(
.
42%
,
,
-
,
,
266
,
,
,
%
2,6%
,
,
,
,
-
.
[11].
-
,
.
.
,
-
[12–18].
[19].
,
,
-
.
Juniperus
sibirica
,
[16, 20, 21].
[22].
Juniperus
sibirica
.
.
Juniperus sibirica
,
1,8–2%.
,
.
Juniperus sibirica
1% (
–
,
,
13
,
-
. 1).
-
.
.
.
.
.
.
77,3%
2,67%
, 21,08%
,
74,63%
.
.
–
1%.
,
,
Juniperus sibirica
.
,
.
-
[23, 24].
,
(
2.
)
.
147
…
1.
Juniperus sibirica
,%
,%
62,02
4,17
2,94
0,17
0,89
0,10
2,04
2,57
1,41
0,99
32-
0,46
2,25
3,63
2,27
9,42
2,81
0,17
0,07
0,43
1,19
,
2.
-
Juniperus sibirica,
.
[14, 25–27].
Juniperus sibirica
%
.*
0,29±0,03
0,09±0,0003
0,31±0,01
0,0018±0,00005
6,77±0,01
–
–
–
–
–
–
,
.
.
.
Juniperus sibirica
1
0,0018%
.
Juniperus sibirica
:
%
–
–
–
–
–
21,78±0,16
4,68±0,14
1,43±0,01
95,42±0,29
126,04±0,25
85,42±0,61
.–
.
[19] (
. 3).
-,
–
,
,
,
,
,
-
.
-
,
,
.
.
,
-
,
.
.
,
[28].
,
,
-
,
,
[29].
,
2,4- (0,2
),
,
(400
-
).
Juniperus sibirica
(
,
4
. 4).
.
,
4,
,
Juniperus sibirica
(37,46%).
(38,17%),
,
.
-
148
.
3.
,
.
,
.
Juniperus sibirica
, /100
3,00
15,00
10,00
1,00
10,00
80,00
200,00
30,00
30,00
5,00
30,00
Pb
Cu
Zn
V
Ni
Ti
Mn
Ba
Sr
Zr
B
.
, /100
<1000
0,03
<1,00
>5,00
5,00
>5,00
0,10
0,20
5,00
>5,00
P
Ag
Y
K
Na
Ca
Al
Fe
Si
Mg
4.
Juniperus sibirica
,%
(40%
)
.
37,46±0,07
13,90±0,08
24,27±0,01
19,13±0,08
4,77±0,06
0,0018±0,00005
6,77±0,01
36,52±0,35
12,46±0,10
23,82±0,14
22,41±0,34
4,50±0,12
0,0038±0,00005
7,50±0,32
,
,
,
.
Juniperus sibirica
.
(
,
Juniperus sibirica
5,
(56,15
.
(1,08
. 5).
,
54,75%
),
-
J. sibirica
(24,12 25,43%).
J.sibirica
1,28%)
-
Pleurotos ostreatus Fr (Kumm).
J.sibirica
Pleurotos ostreatus Fr.
-
6.
,
.
4,7
,
–
,
5,6
.
-
J.sibirica
6,67
7,18%
.
J.sibirica
.
,
,
,
,
.
5.
Juniperus sibirica
,%
(40%
)
9,32±0,07
17,81±0,05
38,34±0,03
27,94±0,08
3,61±0,01
1,08±0,03
.
8,15±0,03
14,03±0,09
40,72±0,12
31,18±0,14
4,01
1,28±0,05
149
…
6.
J.sibirica
,%
.
19,32±0,05
15,13±0,01
33,27±0,11
21,56±0,17
4,41±0,03
5,04±0,07
19,07±0,03
11,37±0,07
37,93±0,01
24,86±0,21
4,93±0,01
7,20±0,05
,
,
Juniperus sibirica,
.
–
,
–
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
,
,
,
,
.
.
. ., 1987. 277 .
.
.,
., 2001. 350 .
//
.
.
.
.,
.
.,
.
., 1982. 184 .
, 2002. 5. . 8–9.
.
//
.
.
:
.
.
, 1982. . 170–171.
.
. ., 2000. 224 .
.
. ., 1978. 175 .
.
Juniperus L.:
,
,
:
. ...
.
.
.
, 2008. 155 .
.,
.
. ., 1973. 360 .
.
.
, 1952. . 108–114.
.
. ., 1992. 160 .
.,
.,
.
//
. 1977. 3. . 3–27.
.,
.
«
»
//
. 1971. 2. . 68–71.
Muranaka T., Miyata M., Ito K., Tachibana S. Production of podophyllotoxin in Juniperus chinensi's callus cultures
treated with oligosaccharides and a biogenetic precursor // Phytochemistry. 1998. Vol. 49, N2. Pp. 491–496.
.
. ., 1987. 191 .
.,
.
:
.
.
. 2603
.
, 1988. 28 .
.
. ., 1954. 135 .
.
. ., 1975. 322 .
.,
.,
.
. ., 1978. 14 .
.,
.,
.
.
, 1996. 358 .
.
.
. ., 1965. 411 .
.,
.,
.
in vitro //
. 2008. . XXV, 1–2. . 68–70.
.
.
, 2000. 664 .
.,
.,
.,
.
: 3- . ., 2001. . 2. 764 .
.
.
Lignum austriacum L.
//
. 2002. 3. . 37–41.
.,
.
// The Biology of Plant In Vitro and Biotechnology: abstracts VIII International Conference.
, 2003. . 88–89.
Anticancer extract // Chem. Eng. (USA). 2000. Vol. 107, N8. Pp. 17.
.
–
,
,
//
. 1999. . 46, 6. . 837–844.
.
:
. ., 1989. 280 .
20
2011 .
6
2011 .
150
.
,
.
,
.
Zyryanova J.V.*, Aeshina E.N., Velichko N.A. CHEMICAL COMPOUND OF A JUNIPERUS SIBIRICA B., CALLUS
TISSUE AND POST-EXTRACTION THE REST
Siberian State University of Technology, Mira Street, 82, Krasnoyarsk (Russia), e-mail: shjlv@mail.ru
The object of research had been chose a Juniperus sibirica, growing on territory of Krasnoyarsk region of Ermakovsky
area. The purpose of the given work was studying of the qualitative and quantitative maintenance of biologically active substances and mineral structure of a Juniperus sibirica. In connection with uniqueness Juniperus sibirica B. and slow growth had
been offered alternative technology of reception of biologically active substances by means of callus tissue.
The maintenance of biologically active substances was defined by the standard techniques used at the analysis of vegetative raw materials.
Research of mineral structure of needles was spent by atomno-adsorbtion plasma spectroscopy.
Identification of the separate components which are a part of essence, was spent with use of methods of a gas-liquid
chromatography and hromato-massspektrometry.
Chemical compound research callus tissue and post-extraction rest Juniperus sibirica B. spent by the techniques accepted
in chemistry of wood and biochemistry of plants.
As a result of the done work the maintenance of the most valuable biologically active substances in needles, callus tissue
and post-extraction rest of Juniperus sibirica B. has been defined Krasnoyarsk region growing in territory. Whence it is possible to draw a conclusion that a Juniperus sibirica – a source of valuable substances for medicine, perfumery, the foodprocessing industry, and the culture of a fabric – the perspective direction, allowing to keep a genofund of valuable tree species.
Keywords: Juniperus sibirica, a chemical compound, biologically active substances, essence, callus tissue, postextraction the rest.
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Received October 20, 2011
Revised December 6, 2011
*
Corresponding author.
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остатки, ткани, состав, каллуснои, сибирской, можжевельник, химические, послеэкстракционного
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