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The Bodian technic as applied to the complicated cell boundaries of the renal epithelium in mammals.

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L l sis well known, the shape of tlic cells in various portions
of tlic renal tubnlcs in mnmnials is frequmitly quite complicated (for a broad statement of the prohlcm sec Zimmermann,
’11). The demonstration of the liig:.hly irregular cell boundaries can asnally he accomplished, a n d thcn with hut partial
succcss, only by specialized technics, which are rather limited
in numhcr. The object of this not? is to acltl to this number
tlic tcchnic recently cleveloped by Bodian (’36, ’37) f o r the
study of the nei’rous system. Tn our litiiids this method like\vi\e has given on17 pa hj- impiq-nation of the cell boundaries, and none of the variations in the technic \?hic*liwe have
en11)loyed has yielclcd impregnation of rno1.e thaii a small
oritp of the tuhules visible in a given section. Neverthe, \\lien s u c c e d i ~ lresults a r c ohtaiizecl the pictiires are so
sharp aiitl so iiistrnctive that the mcthod is to br Iiigkily rocommtwcled a s supplcmentaq- to tliose already arailablc. 7’hc
technic furtlicrmore h a s the clistiiict nclvantage of being applicable t o miterial well fixed by perfusion witli any fixatire,
embccliletl in paraffin, and scctionetl a t a ~ i ydesired thiclmehs.
Figur c 1 illustrates the a p p e a r ~ ~ i c in
c , a Itotiian pl*epnration,2 of the cell 1)oundar.ies in the pars convoluta of the proxiE’elluw of t h e Henry Stiong Denisor1 Jlcc1ir:jl k’onndation.
(’at killwl n i t h jllurniu~itiiigg a 5 ; perfused via thoracic aortz nit11 salt solut1011,
followed bq ‘SUM’; m a l l b l o t k s of c-oltcl ( u p p a r ~ n t l y well perfusccl) then
iiiirnersed in ‘Snsa. f o r :I short tirnc hcfoir deh)dratiou, etc. Prcpar:ition ill
potargol 2 1 Iioiirs.
115
I’IIL
4NA‘lYJMIChl
RECCRP
\OI
72
hO
1
116
Fig. 1 ltnpregiintion of cell bounr1:irii.s iii pars convolutii of proxirnal tubule
of c a t ~ p l i ~ ~ Bodinn
i i .
[Jrepaiation ; l ~ ~ ~ t fsection,
iii
5 p . Cnmcra liirirla driiw
iiig a t X 3150; :IR rcprodnccd, x 2,520.
BODIAN T E CHNL C F O E TXNAL CELL B O C N D A l ~ l E S
117
ma1 tubule of the cat liepliroi~ Tlie figure ~*cpi*ehents
N
selected instance, showing the maximal impregnatioii wliicli
we have been able to obtain. Iii maiiy installers tlie pictnre
is more delic+ate, often cxceediiigly S O , but is always of the
same essential character. The drawing illlistrat es R section
through the rmal epitlieliiim a t a poiiit whrre the t n h l e is
aiiclergoing a rather sharp h i d , so that the cells a r e transectcd at I arying levels k)etween tlie lurneri a i d the 1)usemc.nt
membrane. In the periplieral 1)ortion of the di-awiiiq, particnlarly to the left and helow, as the cell boundai.irs approach tlir
basement meinbrane the impiqiiation is more delicate than
in the central portion. This iiicreasc in delicacy toward the
basement mernbraiie is a ratliei- c~liaracteristi(~
finding.
Thc cell honndai-ies a r e impregiiatcd as rows of mierenly
spaced black dots. That these clots do iiot represent filamentous structures, seen in cross s ion, is obvions from the fact
that (in this and other prepar
11s) the boundaries maintain
the same dotted cliaracter irrespective of tlie plane iii d i c h
they a r e cut. Furtliermor-e, in places wliere o i i ~call study the
sides of the cells iii mi-facc vim-, one finds sheets of clots,
spaced wi tli variable regularity, the intcrsli
dots bei iig appareiitly entirelF iiiiimpregiiated. At the lumiiial surface of the colls the h o u r i d a ~ + i e(trimiual
~
lm*s) a w
likewise dotted.
The vacuolation of the cytoplasm is an eq~rcssioiiof thc.
f a t dropJcts oriqiiially present in the c.ells. Tlie delimitatioii
of these vacuolar spaces is in some instances sliaiyer iii the
OJ-iqinalpi-epamtion thaii iii the tlrawiny. As liaq been amp1:demonstrated, tlie cdls of this portion of the cat ii~plirona i - e
usually ladcii wit11 fat u n c l ~ riiormtil coiidit ions, n.her.eas the
terminal poi-tioii of tlic proximal t i h l e is fat-free (Peter,
'09 ; Zimmcrmann, '11; Foote, ' 3 6 ) .
SL-XMAI~Y
The Kodian technic l o r iien-ous tissues C t m be iisccl siiccessf u l l y f o r tlic impiqpatioii of the complicated eel I bomitlaries
of the reiial epithelium in mammals. The ii)ipi*egiiation is
exceedingly sharp, arid is granular in c*haracter.
I18
A. L. BKAFFLTN A S D .T. ,I. EOOTE
I~I’I’ERATI~RC
(’ITED
HODTAX,D.
1936 A ~ i e ninetliod for
ining n c r i v fibers axid iicr\c ciidiiigs in
niountcd p:uaffin sections. Anat. Rcc., vol. G5, pp. 89-97.
~ _ _ _
1837~ Thr s t a i n i n g of p a ~ a f i nswtions of nrivoiis tissnes n i t h a r t i Lated protnrgol. The role of fiaativrs. Ibid., 701. 69, pp. 153-163.
EWIF, J . .I. 1936 Segmental diff erentintion In the proxjnial eonvoluted tubule
of the rna~ninalianncpliroii. Proc. Woe. Exp. Bicil. and Med., vol. 34,
pp. 196 198.
PETER,K. 1909 Dic Nicrenkaniilchen des Sfenschen iind einiger Siingetiere.
In: Untersuc.hungen uher Ban nnd Entwirkluiig der rU iere, lierausgcgrbcn von Karl Peter. Gustav Fisclier, J e n n . Erstes Heft. h. 1-355.
Z I I V I ~ I I . R M A ~ ‘K.
R ’ , ITr. 1911 Zur Morphologie dcr Hpithclzrllen der Hztugrtier
niere. Arch. f . rnikios. Anat., E d . 78, R. 199-231.
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boundaries, technik, epithelium, mammal, complicated, renar, applied, bodian, cells
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