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InterLabService

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InterLabService #Interlabservice
InterLabService (ILS) presents
AmpliSens® PCR and Real-Time PCR
kits developed and produced by Russian Central Research Institute for Epidemiology
Key features:
wide range of
pathogen detection
high specificity and
sensitivity
ready-to-use reagents
cost-effectiveness
MUL
TIPL
EX
®
AmpliSens MultiPrime Real-Time PCR kit
HCV/HBV/HIV Testing of donated blood
Simultaneous detection of HCV, HBV, HIV-1, and
HIV-2
Enhanced sensitivity compared to serological
analysis
Testing of both individual samples and mini-pools
Possibility of usage in PCR workflow automation
Compatibility with various nucleic acid extraction
solutions
«AmpliSens® HCV/HBV/HIV-FL» Real-Time PCR kit
is intended for simultaneous detection of hepatitis
C virus RNA (HCV), hepatitis B virus DNA (HBV),
human immunodeficiency virus type 1 RNA (HIV-1)
and type 2 RNA (HIV-2) in blood samples by RealTime PCR.
«AmpliSens® HCV/HBV/HIV-FL» was developed by the Russian
Central Research Institute for Epidemiology to provide safety
control tests during blood transfusions, transplantations, and
preparation of blood products. Investigation of plasma samples
combined into mini-pools is also admissible.
Options for automation
• Real-Time PCR provides direct detection of viral RNA/
DNA;
• Plasma samples or their pools are recommended for
analysis. Serum, blood components, biopsy samples, etc.
can also be used;
Soft-hardware complex
for testing of donated blood
• Extraction is performed from large volume of clinical
samples (up to 1 ml) to ensure maximal sensitivity of
the test;
• Analysis is conducted in one tube, targets are independently detected in five or four channels (with or without
HIV-2,correspondingly)
HCV RNA
Green
HIV-1 RNA
Yellow
HBV DNA
Orange
HIV-1 RNA
Crimson
Exogenous internal control
Pooling
Neon 100-1-8 (XIRIL)
Red
Testing of blood - two approaches:
consequent:
PCR analysis is performed only for samples determined as
negative in ELISA.
Nucleic acid extraction
and preparation for PCR analysis
QIASymphony SP/AS (QIAGEN)
cost-effective
time-consuming
parallel:
PCR and ELISA are performed for all plasma samples.
comprehensive
expensive
Amplification and detection
Rotor-Gene Q (QIAGEN)
www.interlabservice.ru/en
Special software for
total process control
and analysis of results
Plasma Pooling
«AmpliSens HCV/HBV/HIV ® -FL» reagent kit can be used for analysis of individual samples as well as for several
plasma samples combined into a mini-pool. Plasma pooling allows reducing price of analysis and increasing laboratory
throughput.
Number of plasma samples recommended for mini-pools is 4-10 with total volume of 1000 μl.
Plasma pooling techniques:
Single pooling – every single sample is a part of only one
pool.
10 pools
100 samples = 10 pools
This way of pooling is
recommended if:
frequency of positive
samples in PCR analysis
is less than 1% (applicable
if samples are negative in
ELISA);
there is no need to decode
mini-pools promptly
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Double pooling (matrix pooling) – every single sample is
a part of 2 pools.
20 pools
100 samples = 20 pools
This way of pooling is
recommended if:
frequency of positive results after PCR analysis
is ≥1% (PCR analysis
is done in parallel to
ELISA);
there is a necessity in
fast decoding of positive
mini-pools
1
11
21
31
41
51
61
71
81
91
2
12
22
32
42
52
62
72
82
92
3
13
23
33
43
53
63
73
83
93
4
14
24
34
44
54
64
74
84
94
5
15
25
35
45
55
65
75
85
95
6
16
26
36
46
56
66
76
86
96
7
17
27
37
47
57
67
77
87
97
8
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28
38
48
58
68
78
88
98
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29
39
49
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Nucleic Acid Extraction
Methods recommended for nucleic acid extraction from blood plasma
Manual methods:
• RIBO-prep – precipitation method (from 100 μl);
• RIBO-sorb – sorbent method (from 100 μl);
• MAGNO-sorb – magnetic bead-based method (from 200 or 1000 μl).
Semi-automatic methods:
• «NucliSENS® easyMAG» workstation («bioMérieux», France) with use of reagent kit EM-plus (from 100-1000 μl).
Automatic methods:
• Neon 100-1-4 workstation (Xiril, Switzerland) for pooling and QIAsymphony SP/AS (QIAGEN, Germany) for nucleic acid
extraction and sample preparation for PCR.
Sensitivity
Analytical sensitivity (detection in a pool*)
Extraction
volume, μl
Extraction method
HCV,
IU/ml
HBV,
IU/ml
HIV-1,
copies/ml
HIV-2**,
copies/ml
100
RIBO-prep
RIBO-sorb
«NucliSENS® easyMAG» + EM-plus
100
50
200
600
200
MAGNO-sorb
50
25
100
300
1000
MAGNO-sorb
«NucliSENS® easyMAG» + EM-plus
QIASymphony Virus/Bacteria Midi Kit
10
5
20
60
*Sensitivity for individual samples is multiply dependent on dilution during pooling
**Reagent kits: Cat.№ TR-V62-Ms and R-V62(RG,Dt)
Advantages of «AmpliSens® HCV/HBV/HIV-FL» Real-Time PCR kit
Multiplex reaction in one tube saves time and costs
Analytical sensitivity and specificity correspond to the highest
worldwide standards
Mini-pools (up to 10 samples in each pool) can be tested along
with individual samples
Combination of reverse transcription and amplification reactions
simplifies the protocol and reduces number of possible
mistakes
Test reveals infected persons even if ELISA-results are negative and
serve as confirming investigation for ELISA-positive samples
Protected RNA extraction controls and DNA amplification controls
allow monitoring of every step of analysis
Automated soft-hardware complex was developed to analyze
samples and interpret results
Real-Time PCR kit was approved in state trials and evaluated at
large blood transfusion stations and transplantation centers
Automatic or manual extraction from 1000 μl of blood plasma
ensures maximal sensitivity
Ordering Information
Cat. №
Name
Description
TR-V62-Ms
(RG,Dt)-CE
Detection of Hepatitis C (HCV) RNA, B (HBV) DNA, HIV-I RNA, and HIV-II RNA (5 optical
«AmpliSens®
channels are required).
HCV/HBV/HIV-FL» Complete set of kits (nucleic acid extraction kit «MAGNO-sorb», reverse transcription and
Real-Time PCR kits) for use with RG and Dt. 100 tests
R-V62(RG,Dt)-CE
Detection of Hepatitis C (HCV) RNA, B (HBV) DNA, HIV-I RNA, and HIV-II RNA
(5 optical channels are required).
«AmpliSens
Reverse transcription and Real-Time PCR kits for use with RG and Dt.
HCV/HBV/HIV-FL»
Kits are recommended for RNA/DNA extraction: MAGNO-sorb variant 1000, reagents
for «NucliSENS® easyMAG» (+ reagent set EM-plus), RIBO-prep, RIBO-sorb. 100 tests
®
Detection of Hepatitis C (HCV) RNA, B (HBV) DNA, and HIV-I RNA (4 optical channels are
required).
«AmpliSens
R-V50-4x
Reverse transcription and Real-Time PCR kits for use with RG, iQ, Mx, and Dt.
(RG,iQ,Мх,Dt)-CE HCV/HBV/HIV-FL»
Kits are recommended for RNA/DNA extraction: MAGNO-sorb variant 1000, reagents for
«NucliSENS® easyMAG» (+ reagent set EM-plus), RIBO-prep, RIBO-sorb. 100 tests
®
MAGNO-sorb
Kit for DNA/RNA extraction from 1000 μl of human plasma and blood serum. Magnetic
bead-based method.
For manual or automated extraction using Neon 100-1-8 (Xiril’s Robotic Workstation).
100 tests
K2-16-200-CE
MAGNO-sorb
Kit for DNA/RNA extraction from 200 μl of human plasma and blood serum. Magnetic
bead-based method.
For manual or automated extraction using Neon 100-1-8 (Xiril’s Robotic Workstation).
100 tests
K2-9-Et-100-CE
RIBO-prep
Kit for RNA/DNA extraction from blood plasma, liquor, saliva, amniotic fluid, and swabs.
Precipitation method. 100 tests
K2-1-Et-100-CE
RIBO-sorb
Kit for RNA/DNA extraction, universal. Silica sorbtion method. 100 tests
К2-15-96-CE
EM-plus
Additional reagent kit for RNA/DNA extraction on automatic station «NucliSENS®
easyMAG» with use of «AmpliSens® HCV/HBV/HIV-FL». 100 tests
K2-16-1000-CE
Olsuf’evskiy per., 8/1, 119021, Moscow, Russia
Tel.: +7 (495) 664 28 84 | Fax: +7 (495) 664 28 89
pcr@interlabservice.ru
www.interlabservice.ru/en
Автор
tatarskiy-80
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