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Znt. J. Cancer: 66,713 (1996)
Publication of the InternationalUnion Against Cancer
Publicationde I'Union InternationaleContre le Cancer
0 1996 Wiley-Liss, Inc.
ERRATUM
BOTZLER,C., KOLB,H.-J., ISSELS,
R.D. and MULTHOFF,G., Noncytotoxic alkyl-lysophospholipid treatment hcreases
sensitivity of leukemic K562 cells to lysis by natural killer (NK) cells. Znt. J. Cancer, 65,633-638 (1996).
The immunoblot portions in Figures 3 and 4 were not readily recognizable. Following are revised figures with legends for these
Figures.
The authors regret the inconvenience.
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FIGURE3 - HSP72 immunoblot from K562 membranes after
nonlethal heat shock and after ET-18-OCH3treatment. Relative
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protein amounts (15 kg) derived from membrane lysates of K562
cells incubated at 37°C (untreated, Lane l), 41.8"C for 2 hr
(followed by a recovery period at 37°C for 2 hr; HS, Lane 2) or
after treatment with 25 pg/ml ET-18-OCH3 at 37" for 2 hr
followed by a recovery period at 37" for 2hr; ET-lS-OHC,, Lane
were run on a 10% SDS-polyacrylamide gel under reducing
conditions and transferred to nitocellulose. The 72kDa heat shock
protein band was demonstrated with an HSP72 specific monoclonal antibody and detected by the ECL system. The immunoblots
were qualified by laser densitometry; the values represent the ratio
of HSP72 levels observed in the membrane of K562 cells exposed
to heat or to ET-18-OCH3relative to the level in untreated cells.
The results are the means of 3 independent experiments; vertical
bars represent SD. (Inset) One representative immunoblot, showing the amount of HSP72 in the membrane of untreated, heat
treated (HS) and ET-18-OCH3treated K562 cells.
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FIGURE 4 - HSP72 immunoblot from cytoplasmic lysates of
K562 cells after nonlethal heat shock or after ET-18-OCH3
treatment. Relative HSP72 protein amounts in the cytoplasm of
K562 cells. Expression of cytoplasmic HSP72 in either untreated
(Lane l),heat treated (41.8"C, 2 hr; followed by a recovery period
at 37°C for 2 hr; Lane 2) or ET-18-OCH3 treated (25 pgiml, 2 hr;
followed by a recovery period at 37°C for 2 hr; Lane 3) K562 cells.
The cell lysates were run on a 10% SDS-polyacrylamide gel under
reducing conditions and transferred to nitocellulose. The 723 kDa
heat shock protein band was demonstrated with an HSP72 specific
monoclonal antibody and detected by the ECL system. The
immunoblots were quantified by laser densitometry; the values
represent SD. (Inset) One representative immunoblot, showing
the amount of HSP72 in the cytoplasm of untreated, heat treated
(HS) and ET-18-OCH3treated K562 cells. Similar results were
obtained after identical treatments and a 6 hr recovery period at
38°C (data not shown).
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