вход по аккаунту


Book Review Cross Electrophoresis its Principles and Applications. By S

код для вставкиСкачать
and routes form the subject matter of volume 22 of this
comprehensive handbook of biochemistry, which, SO t o
speak, gives a chapter by chapter account of the foundations
of a “submolecular biology”. I n the first chapter A . and
B. Pufltnan describe the general ideas and methods of
quantum biochemistry, which is concerned with the quantum
mechanical investigation of the electronic structure of
biologically important molecules. The achievements and
predictions realizable by means of this theoretical treatment
are illustrated by a number of examples. B. Grabe’s new
interpretation of the “energy-rich bond” is, of course, not
mentioned since oniy the literature up t o the beginning of
1966 is referred to. Attempting t o force such a rapidly
developing science into a relatively slowly appearing compendium clearly has its limitations.
The mechanisms of energy transfer are dealt with by Th. Forster in a short section devoted t o the problem of transfer of
free energy between various cell components. A lucid account is given of the theoretical and the experimental approaches t o the resolution of this problem which, of course,
still rely largely on the use of models. Charge transfer
complexes may be involved in conducting energy from one
system t o another in solution and in solids. The points
touched on in the first chapter are discussed in more detail
in the chapters written by E . J . Bullock. The latter also
contain a tabular survey of the complex molecules of most
biological substances, and in particular those of the flavins
and the pyridine nucleotides. The excellently written and well
thought out section by P. Mitchell is likely to prove of
greatest interest t o the biochemist. In 25 pages the principles
of the chemo-osmotic ion translation through membranes
are discussed. T h e four chapters blend,well t o give contemporary treatment of the subject. As the price might lead
one to expect, printing and presentation are faultless.
L. Jnenicke
[NB 721a IE]
Comprehensive Biochemistry. Edited by M . Florkin and E. H .
Stotz. Vol. 28: Morphogenesis, Differentiation and Development. Elsevier Publishing Company, Amsterdam-London-New York 1967. 1st Edit., xii, 276 pp., Dfl. 42.50.
This volume of “Florkin-Stotz” demonstrates that embryology has a t last outgrown its descriptive biology stage and
is beginning t o be understood o n a molecular level as the
chemical and physical parameters of the growing embryo
become accessible to measurement. The contributors to this
volume d o not conceal the fact that the analytical treatment
of these problems is tedious, and that a chemical understanding of the phenomenon of “life” is still far off. As on
every occasion in the past, the editors have succeeded in
finding experienced and skilled specialists t o write about the
selected topics. A . Monroy deals with oogenesis, in which a n
inert mature egg is fertilized and made to grow into a living
organism by making the genetic information fixed in it
capable of being identified. The information is contained in
nucleic acids, the behavior of which during cell differentiation
is described by J . Brachet in analogy t o the better understood
processes in bacteria. E. Scarano and G . Augusti-Tocco deal
with chemical embryology in a most carefully compiled
section which contains 416 references.
The biochemical processes and the metabolic pathway
regulators acting by interaction of macromolecules during
embryogenesis are modeled largely on reactions known to
take place in the adult organism. A n article by T . Yamuda,
which resembles more a review than a critical evaluation,
discusses how the embryonic induction factors effect the
formation of specific cell populations from undifferentiated
prospective material.
The biochemical processes occurring during hormone-induced,metamorphosis of amphibia are described by R . Weber.
This widely embracing work provides interesting interpretations of long known but still uncertain processes which
must operate in conjuction with “an attuned organism”.
Angew. Chem. internat. Edit.
Vol. 7 (1968) I No. 4
The analogous chapter on insect metamorphosis by L. J . Gilbert is an indication of the potential of insect biochemistry
rather than just a compilation of data. This volume is
stimulating in many respects although some of the aspects
are treated subjectively. The bibliography includes very
recent work; unfortunately, however, references t o previous
volumes are somewhat sparse. The neat arrangement of the
work needs no reiteration. Like its predecessors, this volume
has been printed on soft rough paper. The readability is
therefore spoilt, not by glare, but rather by unsharp contours.
L. Jaenicke
[NB 721 b IE]
Cross Electrophoresis, its Principles and Applications. By S .
Nakamura. Elsevier Publishing Co., Amsterdam-LondonNew York 1966. 1st Edit., ix, 194 pp., numerous illustrations and tables, Dfl. 47.50.
In 1953 Grassmann and Hiibner showed that paper electrophoresis by the curtain principle is suitable for detection of
complex formation or association by dyestuffs if the two
components are applied in such a way that the paths taken
by them on electrophoresis cross each other. This principle
was applied by Nakamuru t o horizontal paper electrophoresis,
extended to a two-dimensional technique, and tested on a
series of interacting systems. Thus arose what is called “cross
electrophoresis”. However, a special case, in which the
substances are applied to the strips in parallel streaks so that
the faster-moving component passes over the slower (which
reacts with it), is described by Lung as ‘‘Uberwanderungselektrophorese” (overrunning electrophoresis).
The first 36 pages of this book are devoted to principles and
apparatus, the next 23 pages t o immunochemical use of the
method. It seems t o the reviewer that, particularly in immunological reactions, the usual carrier material (paper) might be
replaced with advantage by agarose plates or acetate foil;
then, similar results are t o be expected t o those obtained by
Ressler’s or Laurell’s method in which the antibodies are
equally distributed in the medium. Later sections of the book
treat the behavior of trypsin inhibitors and the possibilities
of studying enzyme-substrate complexes. 400 citations from
the literature are given in this book, including more than
30 t o work of the author.
For those who can and wish to apply this comparatively
little-used technique to their own problems, this book is a
safe guide, being written in clear language and well set out.
Unfortunately the pictures obtained by means of cross
electrophoresis have no aesthetic appeal!
B . Kickhofen
[NB 675 IE)
The Structure of Inorganic Radicals. By P. W. Atkins and
M . C . R . Symons. Elsevier Publishing Corp., AmsterdamLondon-New York 1967. 1st Edit., x, 280 pp., numerous
illustrations and tables, bound, Dfl. 60.-.
Our knowledge of free radicals, their electronic states and
bonding, has been greatly extended by the use of electron
paramagnetic resonance (EPR). In this work, inorganic
radicals have a special place because their composition is
generally simple. Their electronic states are important for
clarification of the electronic structure of analogous diamagnetic molecules which arise from these radicals by
addition or removal of an electron. In the book under review,
a n attempt is made t o collect the EPR results on inorganic
radicals in a uniform framework, to consider them critically,
and thus t o make them accessible t o a wider circle of readers.
The book begins with a n introduction to the technique of
EPR investigations and t o the methods of preparation and
matrix-isolation of radicals. This forms the basis for a
gradually developing treatment of the types of radical :
electrons in matrices and in solution; ions and atoms in
matrices; and radical molecules containing two, three, four,
or five atoms. A chapter in which generally valid conclusions
Без категории
Размер файла
160 Кб
book, application, cross, review, electrophoresis, principles
Пожаловаться на содержимое документа