# Book Review Gas Chromatography Principles Techniques and Applications. By A. B

код для вставкиСкачатьRapid developments in this field will of course mean that now there are even more recent variants of instruments, which no longer have the faults of their predecessors, and this inevitably overtakes a carefully compiled book such as this. Examples of omissions due to this factor include a detailed account of the electrofocusing technique, rapid analysis of enzymes in acrylamide gel, the more recent gel electrophoresis methods, the theoretical derivations for particle weight determination of proteins by gel filtration, and many others. But the material that is here is first class and extremely useful for any practising biochemist. The individual sections of the hard cover compilation are to be issued as separate paperbacks, which is very sensible, since any experimenter who is using or thinking of using these methods will want the appropriate book at hand. It would also be a good idea if these were just as sturdily bound as the main volume, since it is probable that they will be handed around from one worker to the next. L. Jaenicke [NB 982a IE] Laboratory Techniques in Biochemistry and Molecular Biology. Vol. 2. By T. S. Work and E. Work, NorthHolland Publishing Comp., Amsterdam 1970. 1st ed., 408 pp., bound, Dfl. 75.-. The second volume of “Laboratory Techniques” consists of two very timely sections : automatic enzyme determination, and the principles of cellulose-exchanger chromatography. D. B. Roodyn, known as the author and editor of a whole series of extremely valuable monographs, here presents a review of over 200 pages of the techniques by which serial analysis and series of analyses needed in modern clinical and biochemical laboratories can be automated and programmed. Although as far as equipment is concerned the author places most emphasis on the Technicon Autoanalyzer, his presentation is by no means one-sided, since he tries to bring out the fundamental principles and to be just to the other methods. This is extremely well done. Descriptions are given of continuous and discontinuous sampling, single and multiple enzyme analyses, serial characterization of the physicochemical parameters of enzymes, data processing, and presentation of the results. In an appendix a FORTRAN program for a general enzyme analysis system is given, together with a summary of all the published automated enzyme determinations up to 1968. For all his enthusiasm for his subject, the author’s presentation is critical and balanced. This is a book by a practical man for the practical man, who will here find an advisor reliable in every respect. Before any chemical research can begin, the substances to be used or investigated must be purified. For complex biological materials and macromolecules, ion exchangers based on cellulose have very recently been introduced and have proved themselves extremely useful. E. A . Peterson is one of the pioneers in this field. His 170-page monograph results from the wealth of experience he has accumulated over the past 15 years. It begins with a description of the cellulose ion exchanger, and then turns to the theoretical foundations of chromatography and the effect of varying the parameters. The next sections, of special value from the practical viewpoint, deal with selection of the chromatographic conditions, preparation of the exchanger, packing and charging of the columns, the actual way in which 586 elution is carried out, and treatment of the eluate. Finally, some current biochemical applications of importance are described. Here too valuable tips are given and the commercially available instruments are listed. The wealth of information given will enable even the novice chromatographer to approach his task with a certain amount of confidence and to make sense of his findings. In both parts of this book the clear presentation, the circumspect and practical arrangement of the material, and the excellent appearance are particularly praiseworthy. This book should be available to every biochemist. L. Jaenicke [NB 982b IE] Gas Chromatography, Principles, Techniques and Applications. By A.B. Littlewood. Academic Press, New York 1970. 2nd. Edit., 546 pp., 152 figures, DM ca. 90.-. The second edition of Littlewood’s book, like the first, is divided into three general sections on principles,techniques, and applications of gas chromatography. The strength of the book lies in the particularly clear and lucid treatment of the theory in Chapters 1-6. However, the discussion of the theoretical relations separately from the applications in the last few chapters will occasionally make it diilicult for the analyst using the book to find explanations for practical observations that he is unable to interpret. Moreover, a comprehensive treatment of the diverse applications of gas-chromatographic methods can scarcely be achieved in the space of one textbook. Applications should be discussed in a textbook only to make the relations easier to understand and to facilitate the use of the special literature. This task is approximately fulfilled by the last part of the book. As far as the second part of the book is concerned, the reviewer is of the opinion that it would have been better to place the emphasis on other phenomena and relations. The theory and construction of detectors such as the thermal conductivity cell and the argon detectors are described in too much detail. The analyst nowadays buys an instrument or an important part of an instrument, such as a detector, and will only rarely be able to make technical modifications and improvements. The properties of a detector that play a large part in determining the reliability and accuracy of quantitative analyses, such as sensitivity, dead volume, linear dynamic range, drift behavior, and calibration factors are important in this connection. The treatment of the argon detectors, with the possible exception of the electron capture detector, in no way reflects their practical importance, which is decreasing. Chapters 9 and 10 could have been shortened in favor of Chapter 11 in particular. Reaction gas-chromatographic methods and combined methods, column changing techniques, and automation of the analysis and of the evaluation are more important than some relations discussed in Chapters 9 and 10. Finally, the reviewer would ask the publisher to make the print clearer and to draw more attention to important conclusions and basic principles by the use of bold type and indentations. To sum up, we can only say that the strength of the book lies in the clear and easily understandable treatmeni of the theory, though the book can also provide some important hints for practical problems. Gerhard Schomburg [NB 973 IE] Angew. Chem. internal. Edit. 1 Vol. I0 (1971) No. 8

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