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Book Review Immuno Enzyme Techniques in Cytochemistry. By W. D. Kuhlmann

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practical basics of organotransition metal chemistry (Collman, Hegedus: Principles and Applications of Organotransition Metal Chemistry is recommended), to bring university organic chemistry tuition u p to date, especially in
the practical field-The book deserves to sell, to be improved by its authors by the making good of the gaps
which have been mentioned and to be continually updated
by the inclusion of new methods.
Thomas Kauffmann (NB 660 IE]
Organisch-chemisches Institut
der Universitat Munster (FRG)
Die Bleibelastung des Menschen. By G. Lehnert and D .
Szadkowski. Verlag Chemie, Weinheim 1983. vii, 96 pp.,
D M 28.00,-ISBN 3-527-26096-X
This booklet describes in 80 pages the important sources
of lead uptake (described here as lead burden) and their
regulating boundary conditions, the functional changes initiated (“stresses”) and the public health measures necessary (in essence tolerance levels). The actual effects of lead
and therapeutic questions are only touched on cursorily.
The descriptive mode of presentation makes the work suitable for use as a n introduction to the field for non-specialists and less useful for medical and toxicological experts.
Its value lies in the long list of literature references covering the occurrence and possibilities of uptake of lead from
industry, technology, foodstuffs and confections and beverages, in the home and in the air. Daily and annual variations and the possibilities of mobilizing lead in the individual are devoted particular attention. There are no references to guide the interested reader to the larger, more
comprehensive monographs on lead and the effects of
lead, although they are continually borrowed from both
for the text and the diagrams. The MAK for lead is erroneously quoted as 150 pg/m3; since 1977 the value has been
100 pg/m3.
Dietrich Henschler [NB 658 IE]
Institut fur Toxikologie und Pharmakologie
der Universitat Wiirzburg (FRG)
Selectivity- A Goal for Synthetic Efficiency. Workshop
Conferences Hoechst. Vol. 14. Edited by W . Bartmann
and B. M. Trost. Verlag Chemie, Weinheim 1984. xi, 423
pp., bound, D M 85.00.-ISBN 3-527-26142-7
Selectivity is a conditio sine qua non for efficiency in
chemical synthesis. The discovery of new, more efficient
regio-, chemo- and stereoselective methods is thus of particular importance. The advances in this field are so breathtaking that several selective syntheses are often available
now for complex structures that were previously only difficultly accessible. The Achilles heel of many selective methods and syntheses is, however, their lack of practicability.
Biological synthesis is still better than chemical for many
complicated natural products of wide application such as
penicillins, cephalosporins, steroids and macrolides. The
challenge to chemical synthesis is increased by the development of gene technology. It is greatly to be welcomed
that the papers presented by internationally acknowledged
specialists at the 14th Workshop Conference Hoechst are
now available in book form and have thus been made
available to a wider, interested public. The following individual topics are treated:
Stereoselectivity in the Synthesis of Cyclic Ethers (P. A .
Bartlett); Some Uses of Silicon-Nitrogen Bonds in Organic
Synthesis ( R . J . P. Corriu and J . J . E . Moreau); Designed
Complexes-Science and Applications (0.J. Cram and J.
M. Cram); Asymmetric Synthesis of Carbonyl Compounds
and Primary Amines (0.
Enders); Stereoselection by Allylboronates. The Highs and the Lows (R. W . Hoffnzann); Palytoxin (Y. Kishi); Tris(dia1kylamino)sulfonium Enolates
and Phenoxide ( R . Noyori); Regio-, Diastereo- and Enantioselective Carbon-Carbon Bond Formation-Recent
Studies and Applications in Organic Synthesis (W. Oppolzer); Selectivity and Reactivity in Oligosaccharide Synthesis
(H. Paulsen); Organotin Reagents for Selective Reactions:
Synthesis and Applications of a-Heterosubstituted Organotins ( M . Pereye, B. Elissondo und J.-P. Quintard); Chiral
Building Blocks in Natural Product Synthesis (G. Quinkert); Regioselectivity in Metal-Promoted Carbon-Carbon
Coupling Reactions (M.F. Semrnelhack); Strategies for
Controlling the Stereocheniical Course of Yeast Reductions (C. J . Sih, B. Zhou, A . S . Gopalan, W.-R.Shieh and F.
Van Middlesworth) ; Conformational Control of Chemical
Reactions ( W . C . Still) ; Radical-Mediated Cyclisation
Processes (G. Stork); Diastereoselectivity and Asymmetric
Induction in the Diels-Alder Reaction with Inverse Electron Demand (L. F. Tietze) ; New Palladium-Catalyzed
Reactions of Allylic Compounds via n-Allylpalladium
Complexes (J. Tsuji); Selectivity in Excited State Chemistry (P. A . Wender); Chiral Building Blocks in Alkaloid
Synthesis (E. Winterfeldt); Selectivity in Organic Synthesis
( H . Wynberg); On the Mechanism of Titanium-Tartrate
Catalyzed Asymmetric Epoxidation ( K . B. Sharpless, S. S .
Woodard and M . G. Finn); Selectivity in Chemical and Enzymatic Synthesis (J. E. Baldwin).
The contributions, of which several have been published
in the meantime in other places in this or even in an extended form, are in their majority very clearly and synoptically written. Comprehensive reference lists make it possible to become further engaged in the widely ranging
topics. The book is to be recommended to all who stand
somewhat at a distance from this field and wish an overview-albeit incomplete-of the present state of this most
topical field of organic chemistry. The purchase of this
book which offers valuable stimulation with its collection
of the latest results from twenty-two research groups
would also pay the preparative organic chemist working in
this field. However, the price seems rather high for a n extract-like “snapshot” of a rapidly developing field.
Hans-Joachim Gais [NB 657 IE]
Institut fur organische Chemie und
Biochemie der Technischen
Hochschule Darmstadt (FRG)
Immuno Enzyme Techniques in Cytochemistry. By W . D .
Kuhlmann. Verlag Chemie, Weinheim 1984. xiii, 162 pp.,
bound, DM 128.00.-ISBN 3-527-26078-1
Advances in fundamental biological and medical research are based to a large extent on the introduction of
new methods. Nowadays, no laboratory working in cell
biology can manage without gene manipulation and/or
immunological techniques. Scientists are always being
confronted anew with the problem of choosing and/or
Anyeu Chem Int Ed Enyl 24 (19RSi No 7
meaningfully adapting the right methods for their particular purposes from the wealth of possible choice. Therefore
practical handbooks fulfill an important purpose and every new publication is eagerly awaited. The work in question-written in English-is divided into eight chapters: 1)
Basic Methods and Aspects in Immunological Reagent
Preparation, 2) Preparation of Immunohistological Reagents, 3) Histological Immuno Enzyme Techniques, 4)
General Preparation of Biological Specimens, 5) Immunoperoxidase Histology in the Light Microscope, 6) Immunohistological Assays for Electron Microscopy, 7) Immunohistological Specificity, 8) Practical Aspects.
The book claims to provide a review of the concepts and
practical aspects of immunohistological methods, in particular those of immuno enzyme techniques, it promises a
detailed description of the preparation and purification of
the necessary reagents, wishes to warn the reader about
making false interpretations and to be a trustworthy manual for all who have to become conversant with immunohistological methods.
Does it fulfill what it promises? The answer, unfortunately, is a very definite no. The reader who attempts to
work through the instructions is confronted with insuperable problems. Who knows offhand what protein A is used
for (p. 28, 44, 45, the explanation follows o n p. 62, only p.
45 is mentioned in the index), or what a n epitope is (p. 32,
an antigen determinant), what type of ape is hiding behind
the name Cercopithecus aethiops (p. 122, long-tailed monkey) and how one obtains its kidney cells, because they are
required for one of the experiments.
The presentations are sometimes too trivial and sometimes too specialized for the scientist with years of laboratory experience. Unfortunately there is no general review
of the immuno enzyme techniques, but merely a glimpse of
the work being carried out in the author’s laboratory. N o
details are given of how the results he has obtained may be
applied to other systems; the results were sometimes obtained under extremely lavish conditions. Many of the details are imprecise; for example, it is not clear from the text
nor from the legends to Figures 1- 10 and 1 - 15 what protein is yielded by the kinetics or separation illustrated. Yet
this information is essential, for these results only hold for
a particular protein; they cannot be applied to others. In
addition, data are given about the acrylamide concentrations to be used in producing gels (p. 26), but no mention
is made of the fact that gels with these concentrations are
only suitable for proteins of a particular size range. Nowadays, apparatus for polyacrylamide gel electrophoresis
(often home-made) is part of the standard equipment but
what laboratory has the chamber made of vitreous silica
sheet? Normal glass or plate glass are usual. Vitreous silica
is much too expensive. The suggested dimensions
(18 x 9 x 0.3 cm3, p. 26) are intended for special cases. As
shown by the results in Figure 1-19 these proved to be
quite unsuitable for the separation presented since the voltage drop over 18 cm is much too high to achieve a n optimal separation of the material to be separated. A “slotformer” is a chamber-shaped piece of plastic which looks
quite different than is shown in Figure 1-18.
It remains unclear as to why the section ”. .. lectin binding technique“ (p. 50/5l) has been inserted. It is to be expected that the reader will know that concanavalin A (a
lectin) binds glucosyl and/or mannosyl residues and that
as a result these must be exposed on the surface of peroxidase molecules. The mention of G O T staining (p. 65) is
just as incomprehensible. There is a lack of information
about the particular advantages of the methods and their
Anyew Chcm Inr
Ed Engl 24 (1985) No 7
field of application. On the other hand, pure gastrin (p. 85)
is used in the performance of a n investigation without it
being explained how this is obtained. The choice of reagents is not “a matter of personal preference” (p. 62), but
depends quite definitely on the aim and the questions
posed. Remarks such as “Then, alternative procedures
must be elaborated” (p. 98) or “Antibody concentrations
and incubation times were chosen by trial and were within
the limits of immunohistological assays” (p. 120) are of
very little value and serve to frustrate the reader.
Every chapter ends with a comprehensive list of references. There are no critical comments concerning the
methods reported in the literature. Neither are there any
persuasive arguments for the superiority of immuno enzyme techniques over other immunological methods, e.g.
the indirect immune fluorescence with FITC as marker.
This process is employed throughout the world and has led
to spectacular results in recent years. Why was this not
honored by even one single mention in the list of references?
The resolution of the figures is frequently disappointing.
Figure 5-4 is incomprehensible. There are no points of reference or orientation. It is not possible to make out what is
supposed to have been tagged. In many other figures (5-1,
5-2, 5-7 etc.) comparable tissue sections (partial figures)
are reproduced at different magnifications-which is also
confusing. What a pity-an opportunity has been wasted.
Here it has only been possible to highlight a few of the aggravating deficiencies. One thing remains to be said: the
price cannot in any way be justified.
Peter uon Sengbusch [NB 661 IE]
Institut fur Allgemeine Botanik
und Botanischer Garten der
Universitat Hamburg (FRG)
Smectic Liquid Crystals. Textures and Structures. By G. W.
Gray and J. W . Goodby. Leonard Hill, Glasgow-London 1984. xxvi, 162 pp., bound, L 46.00.-ISBN 0-24944168-3
Ellis Horwood Series in Analytical Chemistry. Organic
Trace Analysis. By K . Beyerrnann. Ellis Horwood, ChiChester 1984. 365 pp., bound, L 35.00.--ISBN 0-85312638-0
Einfuhrung in die Physikalische Chemie. By H . Labhart.
Teil V: Molekulspektroskopie. 2nd revised edition by E .
Haselbach. Springer-Verlag, Berlin 1984. xi, 156 pp.,
stitched, D M 24.80.--ISBN 3-540-12983-9
Strategies and Tactics in Organic Synthesis. Edited by 7‘.
Lindberg. Academic Press, New York 1984. xiv, 370 pp.,
bound, $ 57.00.--ISBN 0-12-450280-6
Modern Inorganic Chemistry. Series Editor: J . P. Fackler,
Jr. Carbon-Functional Organosilicon Compounds. By V.
Chvalovsky and J . M . Bellama. Plenum Press, New York
1984. xiii, 301 pp., bound, $ 45.00.--ISBN 0-306-41671-9
Topics in Current Chemistry. Series Editor: F. L. Boschke.
Vol. 123: Structural Chemistry. Springer-Verlag, Berlin
1984. vii, 186 pp., bound, DM 88.00.--TSBN 3-54013099-3
Wiley Series in General Heterocyclic Chemistry. Series Editor: E. C . Taylor and A. Weissberger. Physical Methods
in Heterocyclic Chemistry. Edited by R . R . Gupta. John
Wiley, Chichester 1984. xi, 682 pp., bound, L 153.00.ISBN 0-471-09855-8
Food Analysis. Principles and Techniques. Vol. 1 : Physical
Characterization. Edited by D. W . Gruenwedel and J. R .
Whitaker. Marcel Dekker, Basel 1984. 352 pp., bound,
SFr. 166.00.-1SBN 0-8247-7181-8
'Industrial Electrochemistry. By D. Pletcher. Chapman and
Hall, London 1984. xii, 325 pp., paperback, L 14.95.ISBN 0-412-26530-3
Specialist Periodical Reports. Spectroscopic Properties of
Inorganic and Organometallic Compounds. Vol. 16.
Edited by E. A . V. Ebsworth. The Royal Society of
Chemistry, London 1984. 363 pp., bound, X 78.00 (price
reduction for members of the Royal Society of Chemistry).-ISBN 0-85186-143-1
The Chemistry of Heterocyclic Compounds. Series Editor:
A . Weissberger and E. C. Taylor. Azepines. Part I and
Part 11. Edited by A . Rosowsky. John Wiley, Chichester
1984. Part I : xvi, 822 pp., bound, f 222.75.-ISBN 0471-01878-3; Part 11: xix, 889 pp., bound, f 222.75.ISBN 0-471-89592-x
Theilheimer's Synthetic Methods of Organic Chemistry.
Vol. 38: Yearbook 1984. Edited by A . F. Finch. S. Karger, Basel 1984. xxiii, 620 pp., bound, SFr. 578.00.ISBN 3-8055-3817-0
Chemistry of Peptides and Proteins. Vol. 2. Proceedings of
the Fourth USSR-FRG Symposium, Tubingen, FRG,
June 8-12, 1982. Edited by W. Voelter, E. Bayer, Y. A.
Ovchinnikov, and E. Wiinsch. Walter de Gruyter, Berlin
1984. xii, 497 pp., bound, DM 185.00.--ISBN 3-11009580-7
Ion Exchange Technology. Edited by D. Naden and M .
Streat. Ellis Horwood Publishers, Chichester 1984. 742
pp., bound, L 45.00.-ISBN 0-85312-770-0
Rheinisch-Westfalische Akademie der Wissenschaften. Vortrage N 328: P. Starlinger: Transposition: Ein neuer
Mechanismus zur Evolution; K . Rajewsky: Antikorperdiversitat und Netzwerkregulation im Immunsystem.
Westdeutscher Verlag, Wiesbaden 1984. 52 pp., paperback, DM 15.00.-ISBN 3-531-08328-7; ISSN 00665754
The Institution of Chemical Engineers. Symposium Series
No. 89: Fluid Mixing 11. A Symposium Organized by the
Yorkshire Branch and the Fluid Mixing Processes Subject Group of the Institution of Chemical Engineers,
Bradford University, April 3-5, 1984. Pergamon Press,
Oxford 1984. 207 pp., bound, f 18.70.-ISBN 0-0803 I41 6-3
NATO AS1 Series. Series C: Mathematical and Physical
Sciences. Vol. 124: Magnetic Resonance. Introduction,
Advanced Topics and Applications to Fossil Energy.
Edited by L. Petrakis and J. P. Fraissard. D. Reidel Publishing Company, Dordrecht 1984. xi, 807 pp., bound,
HF1. 255.00.-ISBN 90-277-1752-4
Allenes in Organic Synthesis. By H . F. Schuster and G. M .
Coppola. John Wiley, Chichester 1984. xiii, 358 pp.,
bound, f 54.95.--ISBN 0-471-87284-9
Proceedings of the Xth IUPAC Symposium on Photochemistry. CCC-Interlaken, Switzerland, July 22-27, 1984.
Symposium Editor: A. M . Braun. Presses Polytechniques
Romandes, Lausanne 1984. XI, 619 pp., paperback, SFr.
75.00.-ISBN 2-88074-064-9
Werkstoff Glas. By K . Kiihne. Akademie-Verlag, Berlin
1984. 287 pp., paperback, DM 35.00.-Order No. 763187-0 (6742)
Institution of Chemical Engineers Symposium Series No.
87: EFCE Event No. 299. ISCRE 8 : The Eighth International Symposium on Chemical Reaction Engineering,
University of Edinburgh, 10- 13 September 1984.
Edited by G. S . G. Beveridge and P. N. Rowe. Pergamon
Press, Oxford 1984. xiv, 826 pp., bound, f 55.00.-ISBN
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