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Book Review Methods of Enzymatic Analysis. Vol. 2. Samples Reagents Assessment of Results. Edited by U. Bergmeyer J. Bergmeyer and M

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metry of the lines of an Hg spectrum recorded at low resolution is caused by pressure broadening of the lines (instead of being caused by the curvature of the image of the
monochromator entry slit in the plane of the exit slit).
Chapters 12 (“Lasers”, 19 pp., 120 refs.) and 13 (“Miscellaneous Sources”, 9 pp., 23 refs.) are probably intended
to justify the general title of the book. The inconsequence
of the author in chapter 12 is quite remarkable, for he
writes a relatively large amount on an infrared laser (CO?
laser), but is satisfied in the case of the argon and krypton
ion laser with the statement that both lasers can lase at various wavelengths in the UV and visible range. Chapter 13
is typical of the style of this book. Here sunlight, the deuterium lamp, synchrotron radiation and the high pressure
mercury lamp all come together under the title “Miscellaneous”.
Chapter 14, (“Ultraviolet Irradiators”, 36 pp., 80 refs.)
contains alongside 80 literature references a list of 58
chosen journal articles (with titles) concerning the design
(or construction) and analysis of photochemical reactors.
Finally chapter IS (17 pp., 72 refs.) treats “Hazards of Ultraviolet Radiation”.
It can be seen from these critical remarks and the contents of the book that this book-in contrast to the very
generally formulated title-is only directed at a very limited circle of readers. This is clearly expressed by the author in the first sentence of his foreword: “This book has
been written primarily for the benefit of those who have a
responsibility for, or an interest in, the design, construction, purchase or operation of the “hardware” associated
with applied photochemistry”. The book is uselesswhether as textbook or as reference source-for everyone
else and particularly for those who work in the field of
photophysics.
Finally it may be useful for the reader to know that there
are two very good older books with similar titles. (I) “Ultraviolet Radiation” by L. R . Koller (Wiley, New York
1965, 3 12 pp.) and (11) “Ultraviolette Strahlen” edited by J .
Kiefer (de Gruyter, Berlin 1977, 661 pp.). Both books were
known to the author of the book that has been reviewed,
but they have obviously only served him as a model for his
choice of title. The application of UV radiation is described in (11) by means of examples; it is thus very suitable for use as a textbook.
Bernhard Nickel [NB 680 IE]
Max-Planck-Institut fur
Biophysikalische Chemie, Gottingen (FRG)
Methods of Enzymatic Analysis. Vol. 2. Samples, Reagents,
Assessment of Results. Edited by U . Bergmeyer. J . Bergrneyer, and M . Grassl. Verlag Chemie, Weinheim 1984.
xvi, 539 pp., bound, D M 215.00 (when ordering all ten
volumes).- ISBN 3-527-26040-4 (complete work)
Publication of Bergmeyer’s 3rd edition of “Methods of
Enzymatic Analysis” proceeds with volume 2 which marks
the transition to practice from the theory presented in volume I.‘*] Volume 2 “covers the three steps in obtaining results from laboratory: the pre-analytical phase, the postanalytical phase, and the analysis itself.” This objective is
achieved in a straightforward and concise manner.
The first chapter in volume 2 deals with specimens and
samples. The first section describes preparation and pro[ * ] C I Anyev
Clirm
1111
Ed Enql 23 (1984) 171
Anqrw. (%em l r i r ELI Enyl. 24 (1985) No. 9
cessing methods for samples in clinical laboratory
sciences, food chemistry, cosmetics and pharmacy. The
next section discusses cell and tissue disintegration and includes methods for animal tissues, subcellular fractionation, microorganisms and plant tissues. The third section
describes and compares methods for protein determination. Throughout this chapter, factors which influence and
interfere with these various methods are described, as well
as ways to avoid potential pitfalls.
Reagents for enzymatic analysis are the focus of the second chapter. This chapter includes a section discussing the
handling of reagents (nomenclature, quality requirements,
storage and stability), and diagnostic kits. The compendium of reagents for enzymatic analysis found in the previous edition has been expanded and is included in this
section; assays and characteristics of 143 enzymes and I19
coenzymes, metabolites and reagents are now described. A
new addition is a section including descriptions and characteristics of standard reference materials for biochemistry.
The third chapter is titled “Evaluation of Experimental
Results.” The first section in this chapter discusses transformation of experimental data into results, end-point determinations, non-linear reaction curves, use of standard
curves, standard substances and their terminology and definitions. Equations useful for calculating metabolite concentrations and enzyme activities from photometry, radiometry and related techniques are included along with
specific illustrative examples. A second section covers the
topic of quality control. After a discussion of errors, precision and accuracy, the principles of quality control and its
applications in clinical chemistry are described. A subsection on the use of statistics in quality control covers basic
concepts, evaluation of results, and curve fitting.
This volume concludes with a useful appendix with lists
of symbols, quantities, units and constants; chemical and
biochemical abbreviations; NAD(P)H absorption coefficients: numbering and classification of enzymes; and a table of atomic weights. This volume should find daily use in
the biochemical laboratory as a handbook of both methods
and data.
George M. Whitesides [NB 676 IE]
Harvard University, Cambridge (USA)
Einfiihrung in die Pharmazeutische Chemie. 2nd completely
revised edition. By 0 . - E . Schultz and J . J . Schneckenburger. Verlag Chemie, Weinheim 1984. xv, 510 pp.,
bound, DM 68.00.-ISBN 3-527-26098-6
At the moment, pharmaceutical chemistry is undergoing
a change into medicinal chemistry. In this process the
main emphasis is moving from the chemical via the biochemical into the medical field. Whether this is an advantage for the subject of pharmaceutical chemistry and the
education of pharmacists really remains to be seen. This
book places the medicament with its synthesis, properties
and analysis in the forefront again. The compounds are
summarized according to their pharmacological properties, but described from a chemical point of view. This system is occasionally abandoned, if this is practically forced
by chemical analogy; thus the antitussive normethadone is
treated alongside methadone which is an analgesic. This is
acceptable because there is a cross-reference under the antitussives. The individual substances are specified under
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