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Book Review Protein and Peptide Analysis by Mass Spectrometry. Edited by J. R. Chapman

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publication describing the example of the
reaction. In addition it is possible to request information about the catalytic
cycle on which the reaction is based and
the relevant reaction parameters (substrate, substitution pattern, nucleophile,
leaving groups, etc.). The catalytic cycle
and the list of reaction parameters can also be found in identical form in the Handbook of Palladium-Catalyzed Organic Reactions.
c) Probably the most effective way of
using the data bank is by means of the
various search facilities that are provided.
These allow one to specify keywords relating to the solvent, catalyst, reaction conditions, ligands, yield, and authors. By
carrying out a search with two or more
simukaneous criteria one can limit the
number of matching reactions that it produces and thus arrive more quickly at the
required data. One can also perform a
search in which these criteria are combined with a chosen reaction scheme described by using the ISIS/Draw facility. In
fact, however, it is often advantageous to
use such a reaction scheme by itself, either
by searching directly for the reaction that
the scheme depicts, or by carrying out a
search to list all those reactions in which
the structures of the educt and product are
present as subunits. In another type of situation one’s interest might lie not in a particular reaction but in reactions that involve a given substrate. In that case the
program allows olie to specify the substrate structure together with a reaction
arrow and obtain a list of all reactions
in which that structure is present, either
in its own right or as a subunit. In a
similar way one can also carry out a
search to find a synthesis giving a particular product.
Thus, the Database of Palladium Chemistry provides a toolkit that affords the
user rapid access to important information of all kinds. Furthermore, the data
bank is to be kept up-to-date by continually adding new reactions, typically at
a rate of 500-700 each year. That
prospect will be of great interest to everyone concerned with organopalladium
Franz Zumpe
Organisch-Chemisches Institut
der Universitat Heidelberg (Germany)
Angen,. Chem. Int. Ed. Engl. 1997,36,No. 20
Protein and Peptide Analyis by Mass
Spectrometry- Edited by J. R. Chapman. (Series: Methods in Molecular
Biology, Vol. 61.) Humana Press,
Totowa, 1996. 350 pp., hardcover
$69.50.-ISBN 0-896-03345-7
J. R. Chapman has already edited several successful books on mass spectrometry. The present book of 21 chapters is
concerned with applications of modern
mass spectrometric methods to the analysis of proteins and peptides.
In the first, introductory chapter P.
Roepstorff gives a short summary of the
past and present development of mass
spectrometry. Here also the work of the
late Michael Barber, ‘father’ of FAB ionization, to whom the book is dedicated, is
honored. Then follows an introduction to
mass spectrometry by Chapman, in which
soft ionization methods, mass analyzers,
and modern techniques such as MS/MS
are explained.
The choice of the topics of the subsequent chapters is well balanced and covers
many current analytical problems in
protein chemistry. However, special problems of synthetic peptide chemistry, such
as the characterization of synthetic peptides or analytical problems of combinatorial chemistry, are not discussed. Some
of the topics dealt with in detail are the
determination of the primary structure of
proteins by tandem mass spectrometry, by
low- and high-energy CID, PSD or ladder
sequencing, mass spectrometric fingerprinting for the identification and characterization of proteins, determination of
post-translational modifications (determination of the disulfide bridges), and the
analysis of gIycopeptides and protein conjugates. Also quantitative aspects are discussed in one chapter (quantitative analysis of neuropeptides) . Further chapters
deal with the secondary structural analysis of proteins, the determination of noncovalent (supramolecular) interactions of
proteins, the computer-assisted analysis
of mass spectra, and the identification of
proteins by means of protein databases.
Since the chapters have been written by
different authors (all of whom are recognized experts in the field of biochemical
mass spectrometry), one might expect
some overlapping and repetition. Fortu-
Verlag GmbH. D-694.51 Weinheim, 1997
nately, however, this is not the case. The
individual chapters are on different and
well-demarcated topics, and repetitions
are rare (one of the few exceptions: a table
containing the masses of the amino acid
residues appears both in Chapter 12 and
in Appendix 111).
In accordance with their increasing importance for protein analysis, the two
modern soft ionization techniques of electrospray ionization (ESI) and matrixassisted laser desorption ionization
(MALDI) are treated most thoroughly.
Nevertheless, the classical methods such
as SIMS und FAB have not been forgotten.
As usual for books of the series Methods in Molecular Biology, each chapter is
organized in sections entitled ‘Introduction’, ‘Materials’, ‘Methods’, and ‘Notes’.
These sections contain much advice and
many practical tips, so that the scientist
with the required analytical equipment at
his disposal can put the particular method
into practice right away in his own laboratory. At the end of each chapter is a bibliography, which also, commendably, includes very recent literature (up to the
year 1996). In addition the appendix contains over 180 further references, most of
them from 1995.
The text is well illustrated by numerous
figures and tables and contains only a few
typing errors (e.g., p. 322, ‘Vat’ instead of
‘Val’; p.327, ‘Tryp’ instead of ‘Trp’). The
subject index could be more detailed.
Some terms, such as ‘FTMS’ or ‘ion trap’,
which occur in the text, are not listed here.
The book provides the protein chemist
with comprehensive information about
the capabilities of modern mass spectrometry for biopolymer analysis. Because it is also helpful for practical applications in the laboratory (for example in
explaining which detergents interfere with
the analysis of proteins by ESI-MS), it
can be recommended unreservedly for all
scientists working in the field of protein
chemistry. Before one picks up this book,
however, one should take seriously the
warning that Chapman gives in the preface: ‘the use of mass spectrometry might
be habit-forming’!
Jorg W Metzger
Institut fur Siedlungswasserbau
der Universitat Stuttgart (Germany)
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