close

Вход

Забыли?

вход по аккаунту

?

Carbohydrate impairment and insulin secretory abnormalities among Macaca mulatta from Cayo Santiago.

код для вставкиСкачать
American Journal of Primatology 11:147-162 (1986)
Carbohydrate Impairment and Insulin Secretory
Abnormalities Among Macaca mulatta From Cay0 Santiago
CHARLES F. HOWARD JR.', MAlT J. KESSLER', AND SUSAN SCHWARTZ2
'Division o Metabolic and Immune Diseases, Oregon Regional Primate Research Center,
Beaverton, Caribbean Primate Research Center, University of Puerto Rico School of Medicine,
Sabana Seca
L
Rhesus macaques (Macaca mulatta) from Cay0 Santiago were examined for
evidence of carbohydrate intolerance indicative of potential development of
non-insulin-dependent diabetes mellitus (NIDDM). Monkeys 6 to > 20 years
from natal Groups J, M, and P, a n AGED Group (all >20 years), and
unrelated monkeys removed from the island in association with other groups
(CAYO), were examined with intravenous glucose tolerance tests (iv-GTT).
Morphometric measurements were made on all tested monkeys. Impairments included fasting hyperglycemia ( > 115 mg/dl), impaired glucose
clearance (K < 2.0%/min), fasting hyperinsulinemia (> 150 pU/ml) or hypoinsulinemia (<20 pU/ml), and insulin secretory abnormalities (>500 pU/
ml or < 75 pU/ml). Natal groups J and M had 31%with impairments, group
P had 0% the AGED group had 45%, and the CAYO group had 33%.
Impaired glucose clearance was usually attributable to a reduced insulin
response. Impairments correlated significantly (p < 0.05) to body weight
and a modified Quetelet index, but not to sex, acute stress, or islet cell
antibodies; the relationships to age could not be delineated in this survey.
Impairments in monkeys are indicative of various stages in the asynchronous development of carbohydrate intolerance leading to NIDDM.
Key words: non-insulin-dependent diabetes mellitus (NIDDM), insulin secretion,
genetics, Modified Quetelet Index, morphometry, islet cell antibody
INTRODUCTION
Spontaneous diabetes mellitus has been reported to occur in several species of
nonhuman primates [Howard, 1983; Howard, 19841. The primate disease has usually
been similar to that classed in human beings as non-insulin-dependent diabetes
mellitus (NIDDM). In humans, NIDDM is associated with hyperglycemia and glucose intolerance, usually obesity, and often aberrations in insulin secretion or utilization [Ganda & Soeldner, 1977; Luft et al, 1981; Fajans, 1981; Defronzo et al, 19831.
There are no consistent lesions in the islets of Langerhans. Although genetic factors
play a major role in the development of NIDDM, the disease is aggravated by
environmental factors. NIDDM cannot be readily induced in laboratory animals. In
Received October 16, 1985; revision accepted May 5, 1986.
Address reprint requests to Dr. Charles Howard, Jr., Oregon Regional Primate Research Center, 505 N.W.
185th Ave., Beaverton, OR 97006.
0 1986 Alan R. Liss, Inc.
148 / Howard, Kessler, and Schwartz
contrast, a n insulin-deficient state, closely analogous to insulin-dependent diabetes
mellitus (IDDM) most commonly found in younger human beings, can be produced
in numerous laboratory animal species by pancreatic beta cell ablation techniques,
eg, surgical removal of the pancreas or use of diabetogenic drugs [Howard, 1983;
Howard, 19841.
Much of the published work on diabetes in nonhuman primates consists of case
reports on individual animals [Sokoloverova, 1960; Valerio et al, 1969; Digiacomo et
al, 1971; Kirk et al, 1972; Jones, 1974; Leathers & Schedewie, 1980; Rosenblum et
al, 1981; Howard, 19831. When investigators have sought NIDDM in primates, their
studies have often focused on the associations of diabetes, obesity, and aging. Hamilton and co-workers [Maller & Hamilton, 1968; Hamilton, 1972; Hamilton et al,
1972; Hamilton & Ciaccia, 19781 found that some rhesus macaques caged for many
years became obese; a few developed metabolic changes associated with diabetes.
Hypothalamic lesions [Ranson et al, 1938; Hamilton & Brobeck, 19631 have also
been used to induce overfeeding, obesity, and diabetes, but lack of predictability in
metabolic developments after lesion placement has prevented this experimental
induction technique from being more widely adopted. Efforts to overfeed macaques
[Hansen, 1979; Hansen & Jen, 19791 or surveys of primate groups [Walike et al,
19771 for possible carbohydrate impairment have produced results of limited value.
Although obesity occurs in several primate species, it is not necessarily accompanied
by carbohydate impairment or diabetes [Kemnitz, 19841.
To be most useful for studies on NIDDM, the disease should develop spontaneously in the animals; the causes should encompass genetic and environmental
vectors analogous to those of their human counterparts; there should be information
available on genetic relationships; and it should be possible to recognize the potential for future development of NIDDM in the animals early enough to permit
longitudinal studies on the etiologies and progression of the disease. The rhesus
macaques maintained on Cay0 Santiago fulfill many of these criteria.
Some anecdotal evidence suggested the occurrence of diabetes among the freeranging rhesus macaques maintained on Cay0 Santiago, Puerto Rico. Loy [1970]
observed “One of the dead females suffered from diabetes mellitus, which may have
been exacerbated by the food shortage”; after observing the diabetic female eat two
lizards, he wondered “. . . whether this female’s diabetes was connected with her
consumption of animal protein.” Sade et a1 [1983] commented that a “. . . genealogy
with two diabetic females showed a n average pattern of early infant mortality.”
Both reports apparently referred to females EK and JJ in group F, a group that later
fissioned to yield groups F, M, P, and 0. None of the reported cases was definitively
diagnosed or confirmed. Spontaneous obesity has also been reported in the Cay0
Santiago population [Rawlins & Kessler, 1982; Kemnitz, 19841. Because of these
observations and because the matrilines are known as far back as six generations,
these macaques seemed ideal for evaluation of carbohydrate impairment and insulin
secretory abnormalities.
MATERIALS AND METHODS
Subjects
Rhesus macaques from India were introduced to the island of Cay0 Santiago in
1938. The colony has been used for a variety of behavioral and biomedical studies
[Rawlins, 1979; Rawlins et al, 1984; Rawlins & Kessler, 19861. Animals have been
removed periodically from the island, but no new stock has been added except
through births. Figure 1, which illustrates relationships among the various natal
groups, modifies and extends information contained in a n earlier report [Sade et al,
19761. Several groups were culled from the island in the early 1970s. By 1976, the
Glucose Intolerance in Macaca mulatta I 149
0
Fig. 1. Interrelationships among natal groups on Cay0 Santiago between 1954 and 1986. Remnants of
groups A, C, E, H, and K were removed after troop size had been significantly decimated through selective
culling of individual monkeys. Intact social groups J,0, M, and P were removed in 1984 and 1985. Group
0 was sold and was unavailable for testing. Solitary males are not included in this illustration.
population had become organized into five naturally formed social groups (groups F,
I, J, L, and M consisting of 17 matrilines) and a small band of peripheral males.
Group 0 formed in 1977 and group P in 1984 as a result of the fission of group F
along matrilineal lines [Rawlins, 19791.
Detailed life histories were kept on each monkey through a daily census [Rawlins & Kessler, 19861. Data on natality, morbidity, mortality, and group affiliation
were recorded by a team of experienced observers. Paternity was not known. Each
troop comprised one to four matrilines with a variable number of associated adult
males. The matrilines consisted of a n adult female, her adult female offspring, and
their juveniles and infants. Females remained with the natal group for life or until
fission occurred. Adult males were often nonnatal members of the group, since most
adolescent males disperse from their natal troop at puberty [Rawlins & Kessler,
19821.
The animals on Cay0 Santiago were provisioned daily with 0.23 kg per monkey
of commercial, high-protein (24-26%) monkey diet (Agway, Inc., Syracuse, NY;
Allied Mills, Inc., Chicago, IL;Ralston Purina Co., St. Louis, MO). They also foraged
on the tropical vegetation and were geophagic [Sultana & Marriott, 19821. Water
was available ad libitum from a n automatic water collection and distribution system. The macaques received no disease prophylaxis, but moribund animals were
permanently removed from the island for treatment or euthanasia [Rawlins &
Kessler, 19821.
Groups J and M were removed from Cay0 Santiago in January and February of
1984, and group P was removed in January of 1985; all were housed at the Caribbean
150 / Howard, Kessler, and Schwartz
Primate Research Center (CPRC) Sabana Seca Field Station near San Juan. Monkeys were caged individually, in outdoor group enclosures (7.6 m2), or in corrals
(0.25-2 ha). Thirty-six mature monkeys captured as part of groups J, M, or P
belonged to other natal groups. Data on these monkeys, all males, were included as
a miscellaneous group designated CAYO. In addition to the monkeys from Cay0
Santiago, other macaques maintained as part of a study on aging (>20 years) at
Sabana Seca were also examined (AGED group).
Monkeys weighed from 6 to 12 kg. Only those at least 6 years old were examined; some were more than 20 years of age. Data in this report are on 93 females
and 57 males; the preponderance of females reflects the availability of defined
matrilines. Thirty-two females were pregnant during a t least one test. Pregnancy
could be detected by palpation at 30 days gestational age. By 80 days, gestational
age could be determined within +5%, as verified later by birth records. Lactation or
the presence of a n infant was usually recorded along with other pertinent information at the time of the iv-G'M7. Examination of birthldeath records also provided
information about whether a female would still have been nursing.
Tests and Assays
Intravenous glucose tolerance test. Monkeys were caged individually overnight; food was removed by 1600, and the macaques were sedated the following
morning between 0800 and 1030 h r with a n intramuscular dose of 12 mg of
ketamine-HC1 (Vetalar@,Parke-Davis, Morris Plains, NJ) per kg of body weight.
Sedation was maintained with additional ketamine as necessary. For a n intravenous glucose tolerance test (iv-GTT), a 7-ml blood sample was drawn (zero time
sample); 3 ml was allowed to clot, centrifuged, and serum was collected and frozen.
The remaining 4 ml was added to a tube containing 10 U of heparin and 0.02 ml of
1 M bemamidine-HCI for collection of plasma. After withdrawal of the zero-time
sample, 0.45 g of glucosekg of body weight (1 ml of D50W glucose solutionkg of
body weight) was injected, and 3-ml blood samples were withdrawn at 10, 20, 30,
45, and 60 min. Serum was analyzed for glucose (Beckman glucose analyzer) and
immunoreactive insulin (IRI) (AmershadSearle radioimmunoassay kit). A K-value
(percent glucose clearance/min) was calculated from the glucose levels in the samples removed from 10 min onward until the glucose concentration was within 10%
of the fasting level; thus, the K values represent only the time of metabolic glucose
clearance. A AIRI value was calculated as the increment of insulin secreted during
the first 10 min (insulin level at 10 min minus that at zero rnin). The AIRI has been
established as a sensitive indicator of the acute insulin response [Howard & Fang,
19841. Plasma was analyzed for glucagon with Unger 04A antibody, a n antibody
that gives results identical to those obtained with the earlier 30K antibody [Howard
& Van Bueren, 19811.
Islet Cell Antibody (ICA) Assay. Neonatal Papio anubis pancreas was fixed in
Bouin's solution, embedded in paraffin, and sections were cut and processed through
organic solvents and rehydration. After a n overnight incubation with Macaca
mulatta plasma at dilutions of 1:4 and 1132,sections were washed, and reactions of
antibodies with islet cell antigens were visualized by the immunoperoxidase technique using diaminobenzidine [Erlandsen et al, 1975; Howard & Fang, 19841. The
reaction was scored as negative or minimal (0to 1) or positive (2 to 3);only scores
of 2 or 3 are reported [Howard & Fang, 19841.
Morphometric measurements. Measurements were taken for a Quetelet index
modified for monkeys (MQD at the time of the iv-GTT [Rawlins et al, 1984; Walker
et al, 19841. Crown-rump lengths (vertex to ischial callousities) were measured with
Vernier anthropometric sliding calipers accurate to 1.0 mm; the macaques were in
Glucose Intolerance in Macaca mulatta I 151
left lateral recumbency. Scales accurate to 0.1 kg were used to weigh animals. The
MQI was calculated a s [body weight (kg)/crown-rump length (ern)]' x 1000. Subcutaneous, abdominal skin folds were measured with skin fold calipers (Cambridge
Scientific Instruments); measurements were made approximately 1 cm above the
navel.
RESULTS
Intravenous Glucose Tolerance Tests
Although fasting hyperglycemia a t > 140 mg/dl is definitive for a diagnosis of
diabetes in human beings, levels of 115 mg/dl or greater in humans [Brunzell et al,
19761 as well as in monkeys [Howard, 19831 are considered evidence of fasting
glucose abnormalities. K values of >2.0%/min are characteristic of the nondiabetic
state. Values of 1.0 to 2.0%/min indicate impaired glucose tolerance (IGT); < l.O%/
min occurred in overt diabetes. Limits of normality for insulin measurements were
established from the data on these macaques. All of the values for fasting IRI and of
AIRI were averaged, even though some values were obviously excessive. After a
mean and standard deviation were computed, those values exceeding the mean by
more than 3 SD were discarded. Values above this limit would constitute only 0.26%
of the total sample frequency; values included four AIRI values of > 800 pU/ml and
three fasting IRI of >400 pU/ml. The second calculated mean for AIRI was established at 216.1 pU/ml with a SD of 141.6 and a SEM of 13.7; the upper limits of
normality for AIRI were set at the mean f 2 SD, ie, a AIRI value of >500 pU/ml
was considered as abnormally great. A similar procedure followed for the fasting IRI
values gave a second mean of 61.5 pU/ml with a SD of 42.9 and a SEM of 4.0.
Fasting IRI values greater than mean + 2 SD, ie, > 150 pU/ml, were established as
abnormal. Fasting hypoinsulinemia has been previously established at < 20 pU/ml
[Howard, 19781. Minimal insulin secretion is also abnormal; a AIRI of <75 pU/ml
was established as indicating impairment (AIRI mean minus 1SD).
Means and limits of glucose and insulin values measured during a n iv-G'M'
were established. Fourteen nonimpaired males and 14 nonpregnant, nonimpaired
females were selected (every third monkey on the lists), and means for K and for
glucose and insulin values at each sampling time during the iv-GTT were calculated.
Results were similar whether K values were averaged or whether K was computed
from the mean of glucose values a t 10, 30, and 45 min; inclusion of the 60-min
sample gave erroneous results since the glucose concentration was close to baseline
by 45 min. The average K for females was 3.91 f 0.32%/min, significantly greater
(p < 0.05) than the average K of 3.04 0.24%/min for males. Fasting insulin levels
were essentially the same in males and females (57.2 f 9.4 vs. 55.1 f 6.5), but the
AIRI of 220.3 i 18.1 in females was greater than the AIRI of 152.4 & 13.1 in males
(p < 0.05). There was a slight difference in secretory patterns; insulin peaked at 10
min in females and then decreased, whereas the insulin in males rose slightly more
by 20 min before diminution.
These average glucose and insulin values measured during a n iv-G'M' are used
in Figure 2 to indicate limits of normality. The vertical shaded area represents the
mean f 1 SD. Since males and females were not identical, the appropriate control
is plotted for each sex. Representative iv-G'M' results are plotted in Figure 2 to
convey some of the more common patterns. Figure 2A (OT Female) shows results
from a diabetic monkey with hyperglycemia, impaired glucose clearance, and virtually no insulin response. In Figure 2B (Z1 Female), insulin secretion was minimal,
but glucose clearance was only slightly impaired. Figure 2C (738 Male) illustrates
impaired glucose clearance and insulin secretion; fasting levels of insulin were
slightly elevated, but insulin response was minimal. Examples of fasting and secre-
+
K = 5.32
A5Female
AIR1 = 28
K=17O
.OOTE
C 738 Male
4oo-
-
300 -
P
g 2
8
0
200-
joo-
n-
4oo-
800-
AIR1 - 66
r=,w
-E
--
F
565 Female
AIR1 = 506
800-
K=081
600 -
600-
-
?
L
E
2
400-
200-
0-
0-
0-
tory hyperinsulinemia are shown in Figures 2D (748 Male) and 2E (A5 Female).
Figure 2F (565 Female) illustrates an uncommon pattern in which there was impaired glucose clearance accompanied by fasting hyperinsulinemia, but with a
gradual diminution in insulin levels.
Table I summarizes the different combinations of impairments by sex and
pregnancy status. Five males and two females had impaired glucose clearance with
no evidence of insulin impairments, although most had fasting insulin values and
AIR1 well below the mean. Fifteen monkeys had decreased glucose clearance associated with minimal fasting insulin and/or AIRI; only one of these was hyperglycemic.
The largest group included 23 monkeys with fasting insulin values or AIRI above
the established limits, but with no evidence of changes in fasting glucose or glucose
clearance.
Glucose Intolerance in Macaca mulatta t 153
TABLE 1. Distribution of Different Impairments by Sex and Pregnancy Status*
Possible impairmentsa
Fasting
A IRI
> FBG
<K
X
X
X
X
X
lRI
<
>
>
<
X
X
X
Males
5
2
2
1
X
X
X
X
X
X
X
-
1
1
1
18
23
6
3
1
4
X
X
Totals
3
-
2
2
2
2
1
8
1
4
1
-
-
X
X
Females
Non-PG
PG
-
*IRI, immunoreactive insulin; PG, pregnant.
aImpairments: <K, glucose clearance of <2%/min; > FBG, fasted blood glucose of > 115 mg/dl; >AIRI,
insulin secretory increment of > 500 pYml; < AIRI, insulin secretory increment of < 75 pU/ml; >fasting
IRI, fasting insulin concentrations of > 150 pU/ml; c fasting IRI, fasting insulin concentrations of < 20
pU/ml.
TABLE 11. Distribution of Impairments by Group, Sex, and Pregnancy Status*
Females
Group
N
J
6
5
0
4
24
39
M
P
Aged
Cay0
Totals
Males
I
2
2
0
2
12
18
(%I)
N
(25)
(28)
(0)
(33)
(33)
(32)
25
4
5
7
0
41
Nonpregnant
I
(%I)
13
4
0
6
0
23
(34)
(50)
(0)
(46)
(0)
(36)
N
6
11
6
0
0
23
Pregnant
I
(%I)
2
3
0
1
0
6
(25)
(21)
(0)
(100)
(0)
(21)
Group
totals
54
29
11
20
36
150
(%I)
(31)
(31)
(0)
(45)
(33)
(31)
*N, nonimpaired I, impaired, which include: glucose clearance of < 2%/min;fasting glucose levels of > 115 mg/dl;
increased or decreased insulin secretory response ( >500 or < 75 pU/ml);increased or decreased fasting insulin levels
(> 150 or < 20 pU/ml);PG, pregnant; (%I), percentage of monkeys with some form of impairment.
Table I1 contains a summary of results within the different groups, separated
by sex and by pregnancy status. Impairments include any abnormalities in fasting
levels of glucose or insulin, insulin secretory response, and/or glucose clearance. The
percentages of the monkeys with impairments were virtually the same in natal
groups J and M and in the CAY0 males. However, the females of group P lacked
any impairments. The AGED group had a slightly greater percentage of those with
impairments, especially in females, but it was not statistically significant.
Average glucagon concentrations were 290 f 74 pg/ml for impaired and 188 f
21 pg/ml for nonimpaired monkeys (p > 0.05). Examination of selected impaired vs.
nonimpaired groups or subgroups produced no significant differences among mean
glucagon values. However, glucagon levels did correlate with the ICA scores. Glucagon levels in monkeys with ICA scores of 0 averaged 163 f 10 pg/ml, whereas
154 / Howard, Kessler, and Schwartz
TABLE 111. Comparisons of Insulin Secretory and Fasting Values Among Normal Males,
and Pregnant and Nonpregnant Females*
Males
Females
Nonpregnant
Nonlactating
Lactating
Pregnant
No.
AIR1 (pU/ml)
Fasting IRI
(pU/ml)
39
143.7 k 9.3"
56.0 k 5.9
41
24
16
23
200.0 f 16.2"
227.8 f 20.1"
216.9 f 30.4"
182.3 f 19.5
48.0 f 3.8
51.6 f 4.9
43.4 6.2
64.6 8.7
*Values are the mean Ifr SEM AIRI is the insulin secretory increment, and fasting IRI is the fasting
insulin concentrations.
"Values differ by p < 0.01 for females vs. males.
monkeys with ICA scores of 2 or 3 had glucagon levels of 240 & 23 pg/ml (p < 0.01).
The prevalence of ICA averaged 30% among both nonimpaired and impaired
monkeys.
Sequential Comparisons
The effects of stress on carbohydrate clearance were assessed in order to differentiate between inherent carbohydrate impairment and changes related to new
caging conditions, handling, or sedation. Of the monkeys in group M tested in
January 1984, soon after removal from Cay0 Santiago, 11were tested again in May
1984. Data were analyzed both by comparing means with the Student's t-test and
by analyzing changes in individual monkeys with the Wilcoxon signed rank test.
Neither the decrease in mean K value from 4.0 f 0.5 to 3.2 f 0.4%/min nor the
increase in AIRI from 148 f 29 to 165 f 46 pU/ml were significant. Changes in
individual monkeys were not significant.
Comparison by Age and Sex
All monkeys were > 6 years. The average age of both impaired and nonimpaired
monkeys in groups J and M was about 11 years. Although the 45% incidence of
impairment in the AGED group was greater than in most other groupings, age
differences were not significant. Sex had no effect on the prevalence of the impairments; 23 of 64 nonpregnant females (36%) and 18 of 39 males (32%) had impairments. There were no apparent differences when specific types of impairments were
compared.
Effects of Pregnancy
Results from pregnant and nonpregnant monkeys are tabulated separately in
Tables I and I1 in order to examine for possible effects of pregnancy on the various
impairments. The 21% incidence of impairments in pregnant monkeys is less than
the 36% in nonpregnant monkeys, but not significantly. Table I11 contains further
comparisons of fasting and secretory insulin values by sex, and by pregnancy and
lactation status for nonimpaired monkeys. The average AIRI values for all females
were greater than the average AIRI values for males; results are consistent with the
group of monkeys selected for analysis of iv-GlT results. Values for nonpregnant
females were different when results were compared to those of the males. Fasting
IRI values did not differ significantly among various groups of monkeys.
Testing individual monkeys when pregnant and again when they are nonpregnant is the only way to establish whether impairments in pregnant females are due
Glucose Intolerance in Macaca mulatta I 155
TABLE IV. Effects of Pregnancy on Metabolic Values*
Nonpregnant
Monkey
No.
514
989
268
K
(%/mid
6.8
4.1
2.4
Pregnant
(mg/dl)
AIRI
(pU/ml)
Fasting
IRI
(pU/ml)
K
(%/mid
FBG
(mg/dl)
AIR1
(pU/ml)
Fasting
IRI
(pU/ml)
46
66
64
254
137
38
149
28
26
4.1
7.0
0.6
57
46
151
519
746
87
90
137
166
FBG
* K , glucose clearance; FBG, fasted blood glucose; AIRI, insulin secretory increment; fasting IRI, fasting insulin
concentrations.
TABLE V. Comparisons of Morphometric Measurements Among Macaca mulatta With and Without
Metahalic Imoairments*
Modified
Quetelet Index
Nonimpaired
Impaired
Males
Femalesb
32.0
20.8
1.5'
+ 1.1"
*
39.4 2.9"
26.8 + 2.4"
Skinfold (cm)
Weight (kg)
Nonimpaired
Impaired
Nonimpaired
Impaired
9.5 f 0.2"
7.2 0.2"
11.0 f 0.4"
8.2 0.3"
6.0 f 0.6
5.9 0.7
6.8 f 0.9
8.0 + 1.4
+
*Values are the mean SEM.
"Valuesbetween nonimpaired and impaired monkeys are significantly different by p < 0.01.
'Nonpregnant females.
to inherent metabolic defects or to the pregnancy. Three such monkeys are presented
in Table IV and are not included in other tabulations. Numbers 514 and 989 from
group M had greater AIRI when pregnant than when nonpregnant; changes were
sufficient to place them in the impaired classification when pregnant. Number 268
was the subject of an earlier report [Kessler et al, 19851. More recent testing showed
her to have normal fasting glucose and glucose clearance, but insulin secretion
remained impaired and fasting IRI was quite low. In addition to the three monkeys
listed in Table IV, four other monkeys tested while pregnant and again when
nonpregnant are included in the data in Tables I, 11, and III; three of these tested
normal under all conditions and one had impairments in iv-G?T during and after
pregnancy.
Morphometric Comparisons
Morphometric measurements for males and nonpregnant females, both lactating and nonlactating, are presented in Table V. The average values for the MQI and
weights were significantly greater for impaired than for nonimpaired monkeys.
Impaired males averaged 1.5 kg more and impaired females 1.0 kg more than their
nonimpaired counterparts (p < 0.01). Skinfold thicknesses of impaired monkeys
were also greater than in nonimpaired monkeys, but not significantly.
Genealogy
Figure 3 shows abbreviated matrilines for the monkeys tested in groups J, M,
and P. Monkeys tested are presented in boldface type; other monkeys are included
to delineate matrilines. Asterisks indicate the presence of impairment, whether of
fasting glucose, glucose clearance, insulin secretion, or fasting insulin levels. Im-
156 / Howard, Kessler, and Schwartz
[GlOUpJ]
073-1-4 (00-001
JJ I-d (00-00)
'OT-I(65- )
092-1-6 !00-001
SO09 I-d IW-00)
Ui-f-d (61-83)
418-m (71- I
6251(14- 1
xo-1-a( 6 3 . ~ 1
24-1-6 167-841
'303md (69-84)
458-1-6 171-84)
824.1 (74. )
940.1R8-
1
563.1-6 (73-84)
'685-1-4 (75.85)
8K-I-d 166-82)
662-Id (I3-81)
A114IIP )
8034 (I4. I
447-1-4 ! I t -791
682-1 (75- 1
TN-I-d (82.77)
9K.M (€4441
561-m(73784-I(16
'327-l(10 I
'924-rn(78-
1
1
I
486-1-6 (72-841
946-1 (787E-m-d (68-85)
I
107-rn(71. )
522 l(72- )
751-1(76- )
656-1(7& )
899-1 (78- )
E w a (56-79)
*P84 (66- )
780-1(76- )
972-m(78- )
022-1-6 (54-00)
W-14 (MI-84)
JI-I-d 161-00)
Jz-1-a (65 oa)
518-1172- )
734-1 ( 7 6 )
'832.1(77. )
*937-1(78- )
6J-I-d (68-80)
'514-1(72- )
8571-6 (77-84)
640m(75- )
293-1(69 )
793-1(77. )
'989-1179- )
517-1-6 (72- 1
740-1(76- )
939-Id (18.81)
994-1 ( 7 9 )
'571-1-6 (73- )
7%-1(77- )
076.1-6 171-00)
KO-I-d (00-001
VL-1(63- )
'6461(75- )
831-m(77- )
ZM-I-d 164.00)
'611-m(74- )
WX-I(M- )
53&m(73- )
Kl-f-d (65-85)
476-f (71- )
700-f (75- )
861-f (77- )
984-f (79- )
789-f (76- )
N2-f (66- )
41 1-f-d (71-82)
649-f (75- )
A73-f (79- )
854-f (77- )
979-f (79- )
840-f (77- )
Fig. 3. Genealogy of groups J, M, and P. The code numbersiletters of the monkeys tested are in boldface
type; the asterisk indicates the presence of impairment. Monkey numbers in light type were not tested,
but have been included in the chart to allow delineation of familial relationships. The year of birth and of
death, where known, are in parentheses. f, female; m, male; d, dead.
pairments appeared frequently throughout both groups J and M, and several matrilines had some increased prevalence. Two of three offspring of WF in group J had
impairments. Only two of five offspring of X J in group J had impairments, but both
of these had at least one offspring with impairments. Two of three offspring of both
518 and YL in group M had impairments. Most striking was the lack of any
impairments found in group P.
Glucose Intolerance in Macaca mulatta I 157
DISCUSSION
There are multiple etiologies for NIDDM in human beings. Commonly, NIDDM
occurs in older people; about 80% are overweight. Many are considered insulinresistant. A current scenario for NIDDM supports a tissue refractiveness to insulin,
involving either the receptor binding or metabolic defects distal to the receptors
[Roth, 1980; Olefsky, 1981; Kolterman et al, 1981; Skyler, 19821. However, fasting or
response hyperinsulinemia is not a consistent finding associated with IGT or diabetes [Defronzo et al, 19831. Fasting insulin levels may remain within normal limits.
Impairment in the acute secretory phase of insulin release is often noticeable in the
early stages preceding diabetes; more severe carbohydrate impairment is associated
with a significant decrease in the amount of insulin secreted Fajans, 1981; Luft et
al, 19811. Insight into the various causes of NIDDM requires identification of specific
subpopulations, as well as recognition of the many metabolic alterations occurring
during the progression toward overt diabetes.
Availability of suitable experimental animals can facilitate studies on NIDDM.
Unfortunately, there are few animals with known heritable conditions analogous to
human NIDDM [Howard, 19841. Among diabetic rodents, the db and ob mouse
strains are perhaps the closest to human NIDDM in the ways that specific metabolic
abnormalities develop [Coleman, 19821. These mice become obese and hyperinsulinemic, and show moderate to severe carbohydrate intolerance depending on genetic
backgrounds [Coleman & Hummel, 19731. Diet can be used in some circumstances
to exacerbate carbohydrate intolerance [Leiter et al, 19831.
The most intriguing data on the natural development of diabetes in monkeys
can be gleaned from a series of papers by Hamilton and co-workers [Maller &
Hamilton, 1968; Hamilton, 1972; Hamilton et al, 1972; Hamilton & Ciaccia, 19781.
Caged, sedentary, well-fed Macaca mulatta were followed as they aged and became
obese, hyperinsulinemic, and eventually diabetic.
The potential for producing diabetic primates has generally not been realized
because there have not been suffkient numbers or they have not been monitored
sufficiently long. Also, a satisfactory breeding situation has never been established
for ongoing, in-depth studies and for provision of monkeys to other investigators
studying diabetes.
The Cay0 Santiago rhesus macaques have been under continuous observation
since 1956. Because new monkeys have been introduced only through births, the
population is relatively homogeneous and matrilineal relationships have been established. Obesity has been documented, and there have been anecdotal comments of
apparent overt diabetes. The recent reduction of the Cay0 Santiago population
through removal of monkeys to the CPRC Sabana Seca Field Station has made
many of them available for biomedical research. One of our primary objectives was
to establish whether these monkeys did have carbohydrate impairment and fasting
or insulin secretory abnormalities, as the early observations suggested, and whether
such abnormalities would be associated with their natal group, matrilines, age, or
body morphometry, especially obesity.
We have identified substantial numbers of macaques with hypo- or hyperinsulinemia and IGT. Just as there are many causaI factors for diabetes in humans,
impairments in these monkeys likely relate to a variety of circumstances.
Genetics does appear to play some role in the appearance of impairments.
Abnormalities appeared commonly in groups M and J. Impairments were more
frequent among several sibling units than among others in these groups. The
absence of impairments among group P monkeys further supports the selective
effects of inheritance on carbohydrate intolerance among these monkeys.
158 / Howard, Kessler, and Schwartz
Morphometric measurements relative to body mass (MQI and weight) did reveal
greater size of impaired monkeys. The Modified Quetelet Index, Obesity Index Rh,
and abdominal skinfold measurements have been found to correlate strongly with
body fat values derived from tritiated water determinations in monkeys [Walker et
al, 1984; Jen et al, 19851. Skinfold measurements were also greater in impaired than
in nonimpaired monkeys, but differences lacked statistical significance; problems
associated with experimental variations in establishing skinfold thicknesses have
been noted [Bray, 19761. Age could not be established as a major contributor to
impairments, although the selection of monkeys in this first phase of the study may
have masked age effects. Impairments were equal between females and males.
Some of the iv-GITs were conducted during January or February, when many
females were pregnant, and in May, when mothers were lactating. Differences in
glucose clearance, fasting insulin levels, and/or insulin secretory responses associated with pregnancy in monkeys have previously been noted, although results were
not consistent among investigators [Pitkin et al, 1970; Mintz et al, 1972; Meis et al,
19821. Rather than differentiate among results obtained during early or late pregnancy, we chose to examine data from all pregnant females as one category. We had
previously reported gestational diabetes in a n aged pregnant female, who also had
IGT postpartum [Kessler et al, 19851. Some of the females in this current group with
apparent abnormalities during pregnancy may eventually be found to have been
gestationally diabetic, as were the three reported here. However, gestational diabetes does not likely account for a significant number of the abnormalities among the
females. Certainly the percentage of abnormalities among nonpregnant females was
greater than among pregnant females, although results were not significantly different. The presence of gestational diabetes in this initial examination merely adds
further credence to our postulate that IGTs have various causes within this group of
rhesus macaques.
An additional concern was whether the observed abnormalities were due to
inherent metabolic aberrations or whether they were stress-induced. Although monkeys were initially in a seminatural, free-ranging environment, conditions on Cay0
Santiago cannot be considered stress-free, eg, social and sexual competition, aggression, and food availability can be major contributors to stress. The stresses of the
new situation at Sabana Seca would include caging conditions affecting freedom of
movement, handling, sedation, etc. Acute stress can impair carbohydrate clearance
[Honjo et al, 1976; Ausman & Gallina, 1978; Streett & Jonas, 19821, presumably
through augmentation of catecholamine secretion, which inhibits the acute insulin
response [Miller & Soeldner, 19691. Certain sedatives and anesthetics can cause
significant insulin secretory changes, although ketamine .HC1 seems to cause only
minimal changes in glucose clearance or in the insulin response [Kemnitz & Kraemer, 1982; Brady & Koritnik, 19851. Monkeys examined with iv-GIT immediately
after removal from Cay0 Santiago and again later showed minimal changes. The
current results do not support imposed stress as a major contributor to the observed
carbohydrate impairments.
The prevalence of ICA has direct bearing on delineation of subpopulations of
impaired monkeys. The cause of diabetes in Celebes black macaques [Sulawesi
crested macaques] (Macaca nigra) is a n islet lesion in which secretory cell deterioration occurs concurrently with deposition of the protein amyloid [Howard, 1972;
Howard, 19781. As the secretory cells deteriorate, released antigens induce ICA
formation; the ICA can be used as a marker for the islet lesion and beta cell loss in
Macaca nigra [Howard & Fang, 19841. Among these Macaca mulatta, the presence
of ICA did correlate with increased glucagon concentrations. Such a n increase would
be expected in light of recent results [Howard, 19861 that showed that quantities of
alpha cells increased during progression of the islet lesion in Macaca nigra. In the
current work, we wanted to examine Cay0 Santiago monkeys for NIDDM in which
Glucose Intolerance in Mucucu rnuluttu I 159
a n islet lesion did not play a role, ie, in which causes for diabetes are associated with
genetics, diet, obesity, receptor defects, and old age. More than 70% of the Cayo
Santiago monkeys are ICA negative and thus are less likely to have a n islet lesion
as the cause for impairments.
Our results can be placed in the context of the asynchronous metabolic changes
that occur during progression toward diabetes. Sequential changes for monkeys with
NIDDM, excluding those with abnormalities due to a n islet lesion or pregnancy,
have been noted by other investigators [Hamilton & Ciaccia, 1978; Hansen &
Bodkin, 19851 and have been further corroborated in our studies. Hyperinsulinemia
or other insulin abnormalities, whether fasting or secretory, can be apparent before
there is overt carbohydrate intolerance; many of the hyperinsulinemic monkeys
were normoglycemic and had normal carbohydrate clearance. Presumably, in those
monkeys as in human beings, the chronic milieu of insulin secretion is sufficient to
prepare the cells for metabolic clearance before arrival of the glucose bolus, ie, cells
are adequately primed so that glucose clearance is seemingly unrelated to insulin
secretion. As the effects of genetics, diet, and obesity contribute further to the
continuation of hyperinsulinemia, impaired carbohydrate clearance or moderate
fasting hyperglycemia may appear. With time, the beta cells lose their ability to
respond with adequate insulin secretion and there is severe fasting hyperglycemia
and marked carbohydrate intolerance. Our survey allowed identification of many
monkeys with tendencies toward further deterioration. Some of these monkeys will
likely progress to a n overtly diabetic state. Through recognition of subpopulations
of monkeys with different causes for carbohydate impairment and in different stages
of development toward diabetes, it will be possible to utilize these macaques to gain
a better understanding of their progression toward diabetes and to establish analogies with similar aspects of the human disease.
CONCLUSIONS
1. Over 30% of the Macaca mulatta removed from Cay0 Santiago as well as
others now housed at Sabana Seca (aged 6 to > 2 0 years) had evidence of carbohydrate intolerance.
2. Carbohydrate impairments, mainly decreased glucose clearance in iv-GlTs,
but also some fasting hyperglycemia, were found in 15% of the monkeys.
3. Insulin aberrations of fasting hypo- or hyperinsulinemia and secretory response abnormalities of either minimal or excessive insulin during iv-GTTs were
present i n 27%of the monkeys.
4. Male and nonpregnant female monkeys with impairments had significantly
greater Modified Quetelet Indexes and body weight than did their nonimpaired
counterparts.
5. The prevalence of impairments does relate to matrilines; impairments appeared often in groups J and M, whereas group P monkeys had no significant
impairments.
6. Neither sex nor islet cell antibodies were associated with the prevalence of
impairments.
7. Age as a contributing factor could not be delineated in this study.
8. Acute stress was not likely a contributor to the carbohydrate impairments.
9. Monkeys exhibited several metabolic abnormalities analogous to those observed during development of non-insulin-dependent diabetes mellitus in human
beings.
ACKNOWLEDGMENTS
The work described in this article, Publication No. 1447 from the Oregon
Regional Primate Research Center (ORPRC), was supported by Animal Resources
Branch Grant RR-00163 and General Research Support Grant RR-05694, both from
160 / Howard, Kessler, and Schwartz
the Division of Research Resources, National Institutes of Health. The ORPRC is
fully accredited by the American Association for the Accreditation of Laboratory
Animal Care (AAALAC). Work in the ORPRC laboratories was supported by U.S.
Public Health Service Grants AM-21982, AG-2281, and RR-05694. At the CPRC,
work was supported by U.S. Public Health Services grant RR-01293 to the University of Puerto Rico. Joann Wolff, Tonya Van Bueren, and Fred Feil a t the ORPRC
and Sammy Martinez, Janis Gonzalez, and the staff of the CPRC provided valuable
technical assistance.
REFERENCES
Ausman, L.M.; Gallina, D.L. Response to
tes mellitus. ARCHIVES OF INTERNAL
glucose loading of the lean squirrel monkey
MEDICINE 137:461-469,1977.
in unrestrained conditions. AMERICAN Hamilton, C.L. An observation of long-term
JOURNAL OF PHYSIOLOGY 234:R20experimental obesity and diabetes mellitus
R24, 1978.
in the monkey. JOURNAL OF MEDICAL
Brady, A.G.; Koritnik, D.R. The effects of
PRIMATOLOGY 1:247-255,1972.
ketamine anesthesia on glucose clearance Hamilton, C.L.; Brobeck, J.R. Diabetes melin African green monkeys. JOURNAL OF
litus in hyperphagic monkeys. ENDOCRIMEDICAL PRIMATOLOGY 14:99-107,
NOLOGY 73512-515,1963.
1985.
Hamilton, C.L.; Ciaccia, P. The course of deBray, G. The obese patient, pp 1-450 in MA- velopment of glucose intolerance in the
JOR PROBLEMS IN INTERNAL MEDI- monkey (Mucucu muluttu). JOURNAL OF
CINE, VOL. 9. L.H. Smith, Jr., ed. Phil- MEDICAL PRIMATOLOGY 7:165-173,
adelphia, W.B. Saunders Co., 1976.
1978.
Brunzell, J.D.; Robertson, R.P.; Lerner, R.L.; Hamilton, C.L.; Kuo, P.T.; Feng, L.Y. ExperHazzard, W.R.; Ensinck, J.W.; Bierman,
imental production of syndrome of obesity,
E.L.; Porte, Jr., D. Relationships between
hyperinsulinemia and hyperlipidemia in
fasting plasma glucose levels and insulin
monkeys. PROCEEDINGS OF THE SOCIsecretion during intravenous glucose tolerETY FOR EXPERIMENTAL BIOLOGY
ance tests. JOURNAL OF CLINICAL ENAND MEDICINE 140:1005-1008,1972.
DOCRINOLOGY AND METABOLISM Hansen, B.C. Induction of obesity in nonhu42:222-229, 1976.
man primate models of human obesity. Pp
Coleman, D.L. Diabetes-obesity syndromes in
291-314 in PRIMATES IN NUTRITIONAL
mice. DIABETES 31:l-6, 1982.
RESEARCH. K.C. Hayes, ed. New York,
Coleman, D.L.; Hummel, K.P. The influence
Academic Press, 1979.
of genetic background on the expression of Hansen, B.C.; Jen, K.-L.C. Caloric intake and
the obese gene in the mouse. DIABETOLOweight changes in adult rhesus monkeys.
GIA 9~287-293,1973.
PRIMATES IN NUTRITIONAL REDefronzo, R.A.; Ferrannini, E.; Koivisto, V.
SEARCH, K.C. Hayes, ed., Academic Press.
New concepts in the pathogenesis and treatPp 59-71,1979.
ment of noninsulin-dependent diabetes Hansen, B.C.; Bodkin, N.L. Beta cell hyperresponsiveness to glucose precedes both
melitus. AMERICAN JOURNAL OF MEDICINE 7452-81,1983.
fasting hyperinsulinemia and reduced gluDigiacomo, R.F.; Myers, R.E.; Rivera Baez, L.
cose tolerance in NIDDM. DIABETES
Diabetes mellitus in a rhesus monkey (Mu- 34:8A, 1985.
cuca muluttu): A case report and literature Honjo, S.; Kondo, Y.; Cho, F. Oral glucose
review. LABORATORY ANIMAL SCItolerance test in the cynomolgus monkey
ENCE 21572-574,1971.
(Mucucu ~ ~ . s c ~ c u Z U F ~ S ) . LABORATORY ANIErlandsen, S.L.; Parsons, J.A.; Burke, J.P.; MAL SCIENCE 26:771-776,1976.
Redick, J.A.; Van Orden, D.E.; Van Orden, Howard, Jr., C.F. Spontaneous diabetes in
L.S. A modification of the unlabeled antiMucucu nigru. DIABETES 21:1077-1090,
body enzyme method using heterologous
1972.
antisera for the light microscopic and ultra- Howard, Jr., C.F. Insular amyloidosis and distructural localization of insulin, glucagon,
abetes mellitus in Mucucu nigru. DIABEand growth hormone. JOURNAL OF HISTES 27:357-364,1978.
TOCHEMISTRY AND CYTOCHEMISTRY Howard, Jr., C.F. Diabetes and carbohydrate
impairment in nonhuman primates. F'p 123~666-677,1975.
Fajans, S.S. Etiologic aspects of types of dia36 in NONHUMAN PRIMATE MODELS
betes. DIABETES CARE 4:69-75,1981.
FOR HUMAN DISEASES. W.R. Dukelow,
Ganda, O.P.; Soeldner, S.S. Genetic, acquired
ed. Boca Raton, CRC Press, 1983.
and related factors in the etiology of diabe- Howard, Jr., C.F. Diabetes mellitus: Rela-
Glucose Intolerance in Macaca mulatta I 161
tionships of nonhuman primates and other pathogenesis of maturity onset diabetes. DIanimal models to human forms of diabetes. ABETES CARE 4:58-63,1981.
ADVANCES IN VETERINARY SCIENCE Maller, 0.; Hamilton, C.L. Sucrose and caAND COMPARATIVE MEDICINE 28:115loric intake by normal and diabetic mon149,1984.
keys. JOURNAL OF COMPARATIVE AND
Howard, Jr., C.F. Changes in islet cell com- PHYSIOLOGICAL PSYCHOLOGY 66:444position during development of diabetes in 449, 1968.
Macaca nigra. DIABETES 35165-171, Meis, P.J.; Kaplan, J.R.; Koritnik, D.R.; Rose,
J.C. Effects of gestation on glucose toler1986.
Howard, Jr., C.F.; Van Bueren, A. Immuno- ance and plasma insulin in cynomologous
reactive glucagon in nondiabetic and dia- monkeys (Macaca ~ ~ S C ~ C U Z U F ~ S ) . AMERIbetic Macaca nigra. HORMONAL AND CAN JOURNAL OF OBSTETRICS AND
GYNECOLOGY 144543-545,1982.
METABOLIC RESEARCH 13:203-206,
Miller, R.E.; Soeldner, J.S. Suppression of
1981.
Howard, Jr., C.F.; Fang, T.-Y. Islet cell cyto- portal venous insulin concentration by epiplasmic antibodies in Macaca nigra. DIA- nephrine in the conscious monkey. DIABEBETES 33219-223,1984.
TOLOGIA 5179-182,1969.
Jen, K.-L. C.; Hansen, B.C.; Metzger, B.L. Mintz, D.H.; Chez, R.A.; Hutchinson, D.L.
Adiposity, anthropometric measures, and Subhuman primate pregnancy complicated
insulin levels of rhesus monkeys. INTER- by streptozotocin-induced diabetes mellitus.
NATIONAL JOURNAL OF OBESITY THE JOURNAL OF CLINICAL INVESTIGATION 51:837-847,1972.
9~213-224,1985.
Jones, S.M. Spontaneous diabetes in mon- Olefsky, J.M. Insulin resistance and insulin
action. An in vitro and in vivo perspective.
keys. LABORATORY ANIMAL 8:161-166,
1974.
DIABETES 30:149-162.1981.
Kemnitz, J.W. Obesity in macaques: Sponta- Pitkin, R.M.; Van Orden, D.E.; Reynolds,
neous and induced. ADVANCES IN VET- W.A. Plasma insulin response and glucose
ERINARY SCIENCE AND COMPARA- tolerance in pregnant rhesus monkeys. ENDOCRINOLOGY 86:435-437,1970.
TIVE MEDICINE 28:81-114, 1984.
Kemnitz, J.W.; Kraemer G.W. Assessment of Ranson, S.W.; Fisher, C.; Ingram, W.R. Adiglucoregulation in rhesus monkeys sedated posity and diabetes mellitus in a monkey
with ketamine. AMERICAN JOURNAL OF with hypothalamic lesions. ENDOCRINOLPRIMATOLOGY 3:201-210,1982.
OGY 23~175-181,1938.
Kessler, M.J.; Howard, Jr., C.F.; London, W.T. Rawlins, R.G. Forty years of rhesus research.
Gestational diabetes mellitus and impaired NEW SCIENTIST 82:108-110,1979.
glucose tolerance in a n aged Macaca mu- Rawlins, R.G.; Kessler, M.J. A five-year study
Zatta. JOURNAL OF MEDICAL PRIMA- of tetanus in the Cay0 Santiago rhesus monkey colony: Behavioral description and epiTOLOGY 14237-244,1985.
Kirk, J.H.; Casey, H.W.; Hanvell, Jr., J.F. zootiology. AMERICAN JOURNAL OF
Diabetes mellitus in two rhesus monkeys. PRIMATOLOGY 3:23-39,1982.
LABORATORY
ANIMAL
SCIENCE Rawlins, R.G.; Kessler, M.J. THE CAY0
SANTIAGO MACAQUES: HISTORY, BE22~245-248,1972.
Kolterman, O.G.; Gray, R.S.; Griffin, J.; Bur- HAVIOR AND BIOLOGY. The State Unistein, P.; Insel, J; Scarlett, J.A.; Olefsky, versity of New York Press, Albany, New
J.M. Receptor and postreceptor defects con- York, 1986.
tribute to the insulin resistance in noninsu- Rawlins, R.G.; Kessler, M.J.; Turnquist, J.E.
lin-dependent diabetes mellitus. JOURNAL Reproductive performance, population dyOF CLINICAL INVESTIGATION 68:957- namics and anthropometrics of the freeranging Cay0 Santiago rhesus macaques.
969,1981.
Leathers, C.W.; Schedewie, H.W. Diabetes JOURNAL OF MEDICAL PRIMATOLmellitus in a Die-tailed macaaue (Macaca OGY 13:247-259,1984.
nemestrina). ~OTJRNALOF MEDICAL Rosenblum, I.Y.; Barbolt, T.A.; Howard, Jr.,
C.F. Diabetes mellitus in the chimpanzee
PRIMATOLOGY 9:95-100,1980.
Leiter, E.H.; Coleman, D.L.; Ingram, D.K.; (Pan troglodytes). JOURNAL OF MEDIReynolds, M.A. Influence of dietary carbo- CAL PRIMATOLOGY 10~93-101,1981.
hydrate on the induction of diabetes in Roth, J. Insulin receptors in diabetes. HOSC57BVKsJ-db/db diabetes in mice. JOUR- PITAL PRACTICE 15:98-103,1980.
NAL OF NUTRITION 113:184-195,1983. Sade, D.S.; Chepko-Sade, B.D.; Loy, J.; KapLoy, J. Behavioral responses of free-ranging Ian, J.R. Early infant mortality among freerhesus monkeys to food. AMERICAN ranging rhesus monkeys. AMERICAN
JOURNAL OF PHYSIOLOGY 33~263-271, JOURNAL OF PRIMATOLOGY 4:361,
1970.
1983.
Luft, R.; Wajngot, A.; Efendic, S. On the Sade, D.S.; Cushing, K.; Cushing, P.; Dunaif,
162 / Howard, Kessler, a n d Schwartz
J.; Figueroa, A.; Kaplan, J.R., Lauer, C.; related behavior of rhesus monkeys (MaRhodes, D.; Schneider, J. Population dy- caca mulatta) on Cay0 Santiago island,
namics in relation to social structure on Puerto Rico. INTERNATIONAL JOURCay0 Santiago. YEARBOOK OF PHYSI- NAL OF PRIMATOLOGY 3:338,1982.
CAL ANTHROPOLOGY 20:253-262,1976. Valerio, D.A.; Miller, R.L.; Innes, J.R.M.;
Skyler, J.S. Type I1 diabetes: Toward im- Courtney, K.D.; Pallotta, A.J.; Guttmacher,
proved understanding and rational therapy. R.M. Miscellaneous diseases of adults. Pp
101-102 in MACACA MLJLATTA: MANDIABETES CARE 5:447450,1982.
Sokoloverova, I.M. Spontaneous diabetes in AGEMENT OF A LABORATORY BREEDJ
in THEORETICAL ING COLONY. New York, Academic Press,
monkeys. P ~ 171-183
AND PRACTICAL PROBLEMS OF MEDI- 1969.
CINE AND BIOLOGY IN EXPERIMENTS Walike, B.C.; Koerker, D.J.; Goodner, C.J.;
ON MONKEYS. I.A. Utkin, ed. New York, Chideckel, E.W. Assessment of obesity in
pigtailed monkeys. JOURNAL OF MEDIPergamon Press, 1960.
Streett, J.W.; Jonas, A.M. Differential effects CAL PRIMATOLOGY 6:151-162,1977.
of chemical and physical restraint on car- Walker, M.L.; Schwartz, S.M.; Wilson, M.E.;
bohydrate tolerance testing in nonhuman Musey, P.I. Estimation of body fat in female
primates. LABORATORY ANIMAL SCI- rhesus monkeys. AMERICAN JOURNAL
OF PHYSICAL ANTHROPOLOGY 63:323ENCE 32:263-266,1982.
Sultana, C.J.; Marriott, B.M. Geophagia and 329,1984.
Документ
Категория
Без категории
Просмотров
4
Размер файла
1 147 Кб
Теги
secretory, cayo, impairments, mulatta, abnormalities, carbohydrate, among, macaca, insulin, santiago
1/--страниц
Пожаловаться на содержимое документа