Histochimie animale methodes et probl├иmes. By L. Lison. With preface by Pol G├йrard. Gauthire-Villars Paris. 1936код для вставкиСкачать
BOOK REVIEW I-IISTOCHIMIE ANIMALE METHODES ET PROBL~CMES.BY L. Lison. With preface by Pol GBrard. Gauthire-Villars, Paris. 1936. This volume appearing as a part of the Collection des ActualitEs Biologiques under the direction of 31.Robert Levy forms a n important contribution to histochemistry. It is especially timely in that there would seem to be an awakening interest in this peculiarly fundamental yet frequently misguided branch of histology. Advances have followed the development of essentially new methods and in the last few years we have had made available several sound techniques, From the papers which have followed as a result of these methods it is apparent that histochemistry is again in a n ascending phase of its ageless cycle. 31.L. Lison has laboriously collected from the literature many of the accepted methods for the demonstration of various substances in tissue sections. Better yet he has tried them, altered them and has rendered a critical opinion as to their value. He has laid down several fundamental requirements of all histochemical techniques. Among these the most important are “la conservation exacte de la localisation de la substance recherchke” and “La mise en Gvidence de fagon adBquate et spe’cifique de la substance envisagbe. ” M. Lison has been adequately critical of the latter requirement but for many reasons more lenient in holding histochemical procedures to the former one. He has drawn attention in a convincing fashion to the difficulties encountered in obtaining good reactions because of the extremely minute amounts of material available in histologic$ section. The author’s statement that in some instances histochemical reactions are as much as 50,000 times more sensitive than spectrum analysis would seem however to be somewhat enthusiastic. The ambitious scope of M. Lison’s book is indicated somewhat by his chapter titles. These range through micro-spectroscopy, histospectrography, micro-spectro-fluoroscopy, polarized light, microincineration, alkaline metals, heavy metals, anions (some twenty-two methods given and discussed), proteins, products of protein metabolism, nucleoproteins, purines, lipoids, masked lipoids, glucides, peroxydases and phenolases, tyrosinase and dopaoxydase. 501 CKE AX‘ATOJIIOAL RECORD, VOL. 68, KO. 4 502 BOOK REVIEW A little less than one-half of the volume is taken u p with chapters dealing with the general aspects of histochemistry, the study of physical properties of tissues and the mineral elements. These subjects have been, for the most part, accorded satisfactory treatment. The remaining 187 pages are devoted to organic histochemistry, including proteins and their products, lipoids, pigments, etc. Some attention has been given to enzymes and their localization in cells. The bibliography is well selected and carefully presented in subject divisions at the end of each chapter. There is no index but the lack is less felt when one encounters the list of bibliographic abbreviations, the page reference table of authors and the very complete table of contents. While for the most part the book shows every evidence of careful thought and planning it is a little difficult to understand why so much attention has been paid to some particular reactions and so little given to the question of preparing tissues for exanination. For example, seventeen pages are utilized in reviewing and describing methods for the detection of calcium and the Altmann-Gersh frozen dehydration technique is dismissed with a few sentences. That the method is ‘malheureusement fort complexes’ is quite beside the point if it, it is known to do, produces good results. However, M.Lison has produced a most worthwhile and useful book and for it deserves all commendation. GORDON 11. SCOTT.