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Histochimie animale methodes et probl├иmes. By L. Lison. With preface by Pol G├йrard. Gauthire-Villars Paris. 1936

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BOOK REVIEW
I-IISTOCHIMIE ANIMALE METHODES ET PROBL~CMES.BY
L. Lison. With preface by Pol GBrard. Gauthire-Villars, Paris.
1936.
This volume appearing as a part of the Collection des ActualitEs
Biologiques under the direction of 31.Robert Levy forms a n important
contribution to histochemistry. It is especially timely in that there
would seem to be an awakening interest in this peculiarly fundamental
yet frequently misguided branch of histology. Advances have
followed the development of essentially new methods and in the last
few years we have had made available several sound techniques, From
the papers which have followed as a result of these methods it is apparent that histochemistry is again in a n ascending phase of its
ageless cycle.
31.L. Lison has laboriously collected from the literature many of the
accepted methods for the demonstration of various substances in tissue
sections. Better yet he has tried them, altered them and has rendered
a critical opinion as to their value. He has laid down several fundamental requirements of all histochemical techniques. Among these the
most important are “la conservation exacte de la localisation de la
substance recherchke” and “La mise en Gvidence de fagon adBquate et
spe’cifique de la substance envisagbe. ” M. Lison has been adequately
critical of the latter requirement but for many reasons more lenient
in holding histochemical procedures to the former one. He has drawn
attention in a convincing fashion to the difficulties encountered in obtaining good reactions because of the extremely minute amounts of
material available in histologic$ section. The author’s statement that
in some instances histochemical reactions are as much as 50,000 times
more sensitive than spectrum analysis would seem however to be somewhat enthusiastic.
The ambitious scope of M. Lison’s book is indicated somewhat by
his chapter titles. These range through micro-spectroscopy, histospectrography, micro-spectro-fluoroscopy, polarized light, microincineration, alkaline metals, heavy metals, anions (some twenty-two
methods given and discussed), proteins, products of protein metabolism, nucleoproteins, purines, lipoids, masked lipoids, glucides, peroxydases and phenolases, tyrosinase and dopaoxydase.
501
CKE
AX‘ATOJIIOAL RECORD, VOL. 68, KO. 4
502
BOOK REVIEW
A little less than one-half of the volume is taken u p with chapters
dealing with the general aspects of histochemistry, the study of physical properties of tissues and the mineral elements. These subjects
have been, for the most part, accorded satisfactory treatment. The remaining 187 pages are devoted to organic histochemistry, including
proteins and their products, lipoids, pigments, etc. Some attention
has been given to enzymes and their localization in cells.
The bibliography is well selected and carefully presented in subject divisions at the end of each chapter. There is no index but the
lack is less felt when one encounters the list of bibliographic abbreviations, the page reference table of authors and the very complete table
of contents.
While for the most part the book shows every evidence of careful
thought and planning it is a little difficult to understand why so much
attention has been paid to some particular reactions and so little given
to the question of preparing tissues for exanination. For example,
seventeen pages are utilized in reviewing and describing methods for
the detection of calcium and the Altmann-Gersh frozen dehydration
technique is dismissed with a few sentences. That the method is
‘malheureusement fort complexes’ is quite beside the point if it, it
is known to do, produces good results.
However, M.Lison has produced a most worthwhile and useful book
and for it deserves all commendation.
GORDON
11. SCOTT.
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villard, preface, methodes, probl, pol, gauthire, lison, 1936, animals, йrard, paris, иmes, histochimie
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