вход по аккаунту


Human parvovirus b19 dna in synovial fluid.

код для вставкиСкачать
We describe a 33-year-old woman with a serologically proven human parvovirus B19 infection, who
developed synovitis. Using a dot-blot hybridization technique, we detected B19 DNA in her synovial fluid. To
our knowledge, this is the first report of the isolation of
B19 from synovial fluid.
The association of acute viral diseases, such as
hepatitis B, mumps, and rubella, with transient arthritis has for many years been considered as support of
the hypothesis that viruses can be etiologic agents in
chronic arthritides in humans. In particular, infection
with the rubella virus is known to produce an acute
synovitis, which although normally self-limited, has
been reported to recur months or years after the acute
stage in some cases (1,2). During recent years, attention has been focused on the human parvovirus B19;
infection with this virus, too, can lead to arthralgia or
frank arthritis, especially in adult women (3,4). However, there is only l previously published report of an
attempt to isolate B19 from synovial fluid; in that
study, B19 DNA was sought in the synovial fluid of 1
patient, but was not found (5). We report here our
From the Departments of Rheumatology and Virology,
University Hospital, Leiden, The Netherlands.
B. A. C. Dijkmans, MD: Department of Rheumatology;
A. M. W. van Elsacker-Niele, MD: Department of Virology;
M. M. M. Salimans, PhD: Department of Virology; G. A. van
Albada-Kuipers, MD: Department of Rheumatology ; E. de Vries,
PhD: Department of Rheumatology; H. T. Weiland, MD: Department of Virology.
Address reprint requests to B. A. C. Dijkmans, MD, S t d
Centre Rheumatology, Building 1, C2-Q, Leiden University Hospital, PO Box 9600, 2300 RC Leiden, The Netherlands.
Submitted for publication April 10, 1987; accepted in revised form July 9, 1987.
Arthritis and Rheumatism, Vol. 31, No. 2 (February 1988)
findings of B19 DNA in the synovial fluid of a patient
with a serologically proven B19 infection.
Case report. The patient, a previously healthy
33-year-old woman, was referred to the outpatient
clinic of the Department of Rheumatology, Leiden
University Hospital, because of pain in her right shoulder, wrist, knee, ankle, and the proximal interphalangeal (PIP)joints of both hands. These arthralgias had
developed within 24 hours after the onset of an acute
febrile illness with a transient rash. On examination, no
abnormalities were found, except for swelling of both
knees and of the PIP joints of both hands. The findings
of radiographs of the hands and feet were normal.
The patient’s erythrocyte sedimentation rate
was 23 mm/hour (Westergren), the hemoglobin level
was 8.3 mmolesfliter, and the white blood cell count
was 5.0 x 109/liter with a normal differential cell
count. The results of Rose-Waaler and latex fixation
tests were negative; antinuclear antibody was borderline positive. HLA-DR4 was absent. Serologic studies
ruled out recent infection with the rubella virus. However, a serum sample obtained when the patient was
first seen contained B 19-specific IgM antibodies, as
detected by radioimmunoassay (18 arbitrary units;
normal 5 5 [6]; determined by Dr. M. J. Anderson,
London, UK) indicating recent B 19 infection. Analysis of synovial fluid from the right knee showed a white
blood cell count of 6.5 x 103/mm3,with 66% mononuclear cells; crystals were not seen, and no microorganisms could be cultured.
A dot-blot hybridization technique was used to
examine the synovial fluid for B19 DNA, after reduction of viscosity by hyaluronidase treatment (7). This
technique uses single-stranded, labeled DNA as a
probe to detect complementary DNA. DNA was ex-
tracted from the synovial fluid, and B19 DNA was
found with a 32P-labeled B19 probe (8) (Figure 1)
which was kindly provided by Dr. M. J. Anderson.
Synovial fluid from a control patient whose serum
contained no B19 IgM was treated in the same manner,
and gave negative results (Figure 1). Our patient’s
arthralgias and general malaise persisted for 3 months,
but she recovered completely.
Discussion. To the best of our knowledge, this is
the first description of the presence of B19 DNA in the
synovial fluid of a patient with a serologically proven
B19 infection. It must be kept in mind that arthritis
seen in natural and experimental B19 infections in
humans develops after the virus has disappeared from
the circulation (9). The presence of B19 DNA in
synovial fluid in the present case suggests joint invasion by the virus. The technique we used does not
show whether the arthritis was due to direct infiltration
by the virus or to an immunologically mediated phenomenon.
During recent years, parvoviruses have received
attention in connection with the etiology of rheumatoid
arthritis (RA). One of these viruses, designated RA-1,
was isolated from synovial tissue of a patient with RA
and proved to be pathogenic in mice (10). However, the
RA-1 virus is antigenically different from the B19 virus,
and no similarities have been found in restriction enzyme analysis of the RA-1 virus and several known
parvoviruses (10). Attempts to establish a link between
persistent arthritis after B19 infection and RA have
been based on the presence of HLA-DR4, which occurs more frequently in RA patients than in the general
population (11). Reports on the frequency of DR4 in
patients with persistent arthritis after B 19 infection are
conflicting: Klouda et al (12) found an increased frequency, but in a previous study, we did not (13). The
DR4 antigen was absent in our patient.
Our finding of B 19 DNA in the synovial fluid of
a patient with a serologically proven B19 infection
lends support to the possibility that parvovirus infection can play an etiologic role in RA.
I . Chandler JK, Ford DK, Tingle AJ: Persistent rubella
infection and rubella-associated arthritis. Lancet I: 13341335, 1982
Figure 1. Autoradiograph of a nitrocellulose filter, showing DNA
isolated from 4 samples. A l , DNA from the synovial fluid of a
patient who was seronegative for B19 IgM. B1, DNA from the
synovial fluid of the patient described in the present report, who was
seropositive for B19 IgM. A2, DNA from the serum of another
BIPinfected patient (positive control). B2, DNA from the serum of
a patient who was seronegative for B19 IgM (negative control).
2. Grahame R, Armstrong R, Simmons N, Wilton JMA,
Dyson M, Laurent R, Millis R, Mims CA: Chronic
arthritis associated with the presence of intrasynovial
rubella virus. Ann Rheum Dis 42:2-13, 1983
3. Reid DM, Reid TMS, Brown T, Rennie JAN, Eastmond
CJ: Human parvovirus-associated arthritis: a clinical
and laboratory description. Lancet I: 422-425, 1985
4. White DG, Woolf AD, Mortimer PP, Cohen BJ, Blake
DR, Bacon PA: Human parvovirus arthropathy. Lancet
I: 419-421, 1985
5 . Rowe IF, Deans AC, Midgley J, Anderson MJ, Keat
AC: Parvovirus infection in hospital practice. Br J
Rheumatol 26: 13-16, 1987
6. Cohen BJ, Mortimer PP, Pereira MS: Diagnostic assays
with monoclonal antibodies for the human parvoviruslike virus (SPLI’). J Hyg (Lond) 91:113-130, 1983
7. Clewley JP: Detection of hum,n parvovirus using a
molecularly cloned probe. J Med Virol 15:173-181. 1985
8. Anderson MJ, Jones SE, Minson AC: Diagnosis of
human parvovirus infection by dot-blot hybridization
using cloned viral DNA. J Med Virol 15:163-172, 1985
9. Anderson MJ, Higgins PG, Davis LR, Willman JS,
Jones SE, Kidd IM, Pattison JR, Tyrrell DAJ: Experimental parvoviral infection in humans. J Infect Dis
152:257-265, 1985
28 1
10. Simpson RW, McGinty L, Simon L, Smith CA, Godzeski
CW, Boyd R: Association of parvoviruses with rheumatoid arthritis of humans. Science 223:1425-1428, 1984
1 1 . Stastny P: Association of the B cell alloantigen DRw4
with rheumatoid arthritis. N Engl J Med 298:869-871,
12. Klouda FT, Corbin SA, Bradley BA, Cohen BJ, Woolf
AD: HLA and acute arthritis following human parvovirus infection. Tissue Antigens 28:318-319, 1986
13. Dijkmans BAC, Breedveld FC, de Vries RRP: HLA
antigens in human parvovirus arthropathy. J Rheumatol
13:1192-1 193. 1986
Arthritis &
Institutes Page Charge
Effective immediately, all articles accepted for publication in Arthritis & Rheumatism, which were
submitted on or after January 1, 1988, will be subject to a page charge of $70 per page. Expansion
of the journal has necessitated this new policy. Authors will receive instructions for payment when
they receive proofs of the article. Questions regarding the page charge should be directed to A&R's
business office in Atlanta.
Без категории
Размер файла
213 Кб
dna, b19, synovial, human, fluid, parvovirus
Пожаловаться на содержимое документа