Octacalcium phosphate crystals induce inflammation in vivo through interleukin-1 but independent of the NLRP3 inflammasome in mice.код для вставкиСкачать
ARTHRITIS & RHEUMATISM Vol. 63, No. 2, February 2011, pp 422–433 DOI 10.1002/art.30147 © 2011, American College of Rheumatology Octacalcium Phosphate Crystals Induce Inflammation In Vivo Through Interleukin-1 but Independent of the NLRP3 Inflammasome in Mice Sharmal Narayan,1 Borbola Pazar,1 Hang-Korng Ea,2 Laeticia Kolly,1 Nathaliane Bagnoud,1 Véronique Chobaz,1 Frédéric Lioté,2 Thomas Vogl,3 Dirk Holzinger,3 Alexander Kai-Lik So,1 and Nathalie Busso1 Objective. To determine the mechanisms involved in inflammatory responses to octacalcium phosphate (OCP) crystals in vivo. Methods. OCP crystal–induced inflammation was monitored using a peritoneal model of inflammation in mice with different deficiencies affecting interleukin-1 (IL-1) secretion (IL-1 ␣ –/– , IL-1 ␤ –/– , ASC –/– , and NLRP3–/– mice) or in mice pretreated with IL-1 inhibitors (anakinra [recombinant IL-1 receptor antagonist] and anti–IL-1␤). The production of IL-1␣, IL-1␤, and myeloid-related protein 8 (MRP-8)–MRP-14 complex was determined by enzyme-linked immunosorbent assay. Peritoneal neutrophil recruitment and cell viability were determined by flow cytometry. Depletion of mast cells or resident macrophages was performed by pre- treatment with compound 48/80 or clodronate liposomes, respectively. Results. OCP crystals induced peritoneal inflammation, as demonstrated by neutrophil recruitment and up-modulation of IL-1␣, IL-1␤, and MRP-8–MRP-14 complex, to levels comparable with those induced by monosodium urate monohydrate crystals. This OCP crystal–induced inflammation was both IL-1␣– and IL-1␤–dependent, as shown by the inhibitory effects of anakinra and anti–IL-1␤ antibody treatment. Accordingly, OCP crystal stimulation resulted in milder inflammation in IL-1␣–/– and IL-1␤–/– mice. Interestingly, ASC–/– and NLRP3–/– mice did not show any alteration in their inflammation status in response to OCP crystals. Depletion of the resident macrophage population resulted in a significant decrease in crystal-induced neutrophil infiltration and proinflammatory cytokine production in vivo, whereas mast cell depletion had no effect. Finally, OCP crystals induced apoptosis/necrosis of peritoneal cells in vivo. Conclusion. These data indicate that macrophages, rather than mast cells, are important for initiating and driving OCP crystal–induced inflammation. Additionally, OCP crystals induce IL-1–dependent peritoneal inflammation without requiring the NLRP3 inflammasome. Supported by the Fonds National Suisse de la Recherche Scientifique (grant 310030-130085/1) and the Jean and Linette Warnery Foundation. Drs. Ea and Lioté’s work was supported by grants from the Fondation pour la Recherche Médicale, the Association pour la Recherche en Pathologie Synoviale, and the Association Rhumatisme et Travail. Dr. Holzinger’s work was supported by a grant from the German Ministry of Education and Research (BMBF project AID-NET). 1 Sharmal Narayan, PhD, Borbola Pazar, MD, PhD, Laeticia Kolly, PhD, Nathaliane Bagnoud, MSc, Véronique Chobaz, Alexander Kai-Lik So, PhD, FRCP, Nathalie Busso, PhD: Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland; 2Hang-Korng Ea, MD, PhD, Frédéric Lioté, MD, PhD: INSERM UMR-S606, Hôpital Lariboisière, Assistance PubliqueHôpitaux de Paris, and Université Paris Denis Diderot, Paris, France; 3 Thomas Vogl, PhD, Dirk Holzinger, MD: University of Münster, Münster, Germany. Drs. Narayan and Pazar contributed equally to this work. Address correspondence to Nathalie Busso, PhD, Division of Rheumatology, DAL, Laboratory of Rheumatology, CHUV, Nestlé 05-5029, 1011 Lausanne, Switzerland. E-mail: Nathalie.Busso@ chuv.ch. Submitted for publication May 3, 2010; accepted in revised form November 4, 2010. Basic calcium phosphate (BCP) crystals including hydroxyapatite, carbonated apatite, tricalcium phosphate, and octacalcium phosphate (OCP) have long been associated with rheumatic syndromes. BCP crystal deposition occurs most frequently in soft tissue, muscle, and articular sites and can manifest with acute inflammation and tissue degradation. Indeed, the presence of BCP crystals in synovial fluid is more common in 422 OCP CRYSTALS ARE PROINFLAMMATORY IN VIVO patients with more severe osteoarthritis (OA) (1). Furthermore, it has recently been reported that BCP crystal deposition in knee and hip cartilage is associated with end-stage OA (2,3). In the study concerning hip OA (3), the amount of calcification (predominantly BCP crystals) correlated with clinical symptoms and histologic OA grade. The role of inflammation itself in OA disease progression is still uncertain. BCP crystals have also been associated with destructive arthropathies such as the Milwaukee shoulder syndrome (4). However, the mechanisms that underlie the inflammatory reaction induced by BCP crystals remain unclear. In vitro, BCP crystals induce fibroblast proliferation, protooncogene stimulation, production of inflammatory cytokines (interleukin-1 [IL-1] and tumor necrosis factor ␤ ), metalloproteinase production and activation, cyclooxygenase 1 (COX-1), COX-2, and prostaglandin E2 production (5,6), and chondrocyte production of nitric oxide and apoptosis (7,8). In vivo, BCP crystals have been reported to be proinflammatory, inducing neutrophil influx in the rat air pouch model (9). Recently, a role for IL-1␤ has been demonstrated in monosodium urate monohydrate (MSU) crystal– and calcium pyrophosphate dihydrate (CPPD) crystal– induced inflammation; MSU and CPPD crystals are associated with acute gout and pseudogout, respectively. It is not known whether BCP crystals induce inflammation through this pathway. IL-1␤ is a potent inflammatory cytokine, the production of which is tightly controlled at the level of gene expression, proteolytic processing, and secretion (10). Thus, proIL-1␤ protein (protein of 35 kd molar mass) is converted to active IL-1␤ (protein of 17 kd molar mass) mainly by caspase 1, but other leukocyte proteinases such as proteinase 3, elastase, chymase, and granzyme A may also be involved during inflammation (11–16). The activity of caspase 1 is regulated by the inflammasome, an intracellular multicomponent complex that is assembled following cellular stimuli from pathogens and danger signals (17). Several inflammasome complexes have been described, and activation of the inflammasome has been linked to infectious and autoinflammatory diseases (for review, see ref. 17). NLRP3 is thus far the best characterized inflammasome and is formed by the adaptor protein ASC, caspase 1, and NLRP3 (18). NLRP3 gain-of-function mutations are responsible for one of the hereditary autoinflammatory syndromes, cryopyrin-associated periodic syndrome, that responds dramatically to IL-1 inhibition (19). Similarly, NLRP3 is needed for monocyte IL-1␤ production upon stimulation with MSU and 423 CPPD crystals (20), and studies have demonstrated the clinical efficacy of IL-1␤ blockade in both acute gout and pseudogout attacks (21–23). However, the situation in vivo could be different, as has been suggested by studies in which a role of the inflammasome was not demonstrated in murine models of arthritis that are well known to be IL-1␤ dependent (15). Possible explanations for this discrepancy include the contribution of multiple cell types to the inflammatory state in vivo which is not the case in vitro, and the possibility that crystals exert other effects on tissues to provoke an inflammatory response independent of IL-1␤ production. Finally, crystals may interact with host proteins in vivo to modify their phlogistic effects, as has been demonstrated in MSU crystal–induced inflammation (24,25). This prompted us to assess in vivo the inflammatory effect of OCP crystals using the murine peritonitis model, and to dissect the mechanisms involved in IL-1␤ production. Furthermore, the contribution of the NLRP3 inflammasome in OCP crystal–induced inflammation and peritonitis was investigated. MATERIALS AND METHODS Mice. C57BL/6J mice were purchased from Harlan. IL-1␣–/– and IL-1␤–/– mice were a gift from Dr. Yoichiro Iwakura (University of Tokyo, Tokyo, Japan) (26). ASC–/– mice (27) and NLRP3–/– mice (20) were backcrossed into the C57BL/6J background for at least 9 generations and were compared with wild-type (WT) littermates in this study. Mice were bred under conventional, non–specific pathogen–free conditions. Mice ages 8–12 weeks were used for experiments. Institutional approval was obtained for these experiments. Preparation of MSU and OCP crystals. Sterile, pyrogen-free MSU and BCP crystals were synthesized as previously described (9,20). Crystals were suspended in sterile phosphate buffered saline (PBS) and dispersed by brief sonication. All crystals were determined to be endotoxin free (⬍0.01 endotoxin units/10 mg of crystal) by Limulus amebocyte cell lysate assay. Crystal-induced peritonitis. Mice were injected intraperitoneally (IP) with 1 mg of MSU or OCP crystals in 0.5 ml sterile PBS. To analyze the involvement of IL-1, mice were injected IP with either 10 g of neutralizing rabbit polyclonal anti–IL-1␤ antibody (in 0.5 ml PBS) (Novartis) or 200 g of anakinra (recombinant IL-1 receptor antagonist [IL-1Ra]) (Kineret; Amgen) 30 minutes prior to crystal administration. An equal volume of sterile PBS was injected into control mice. To test the involvement of neutrophil proteinases, mice were treated with 1 mg of the neutrophil elastase inhibitor methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethylketone (AAPV; Calbiochem) 1 hour before crystal administration. (AAPV was initially resuspended in DMSO at 10 mg/ml and diluted in PBS at 1 mg/ml for injections.) Control mice were injected with the vehicle alone. After 6 hours, blood was collected, mice were euthanized by CO2 administration, and 424 peritoneal exudate cells were subsequently harvested by performing lavage with 3 ml of PBS. Total numbers of viable peritoneal exudate cells were determined by trypan blue exclusion. Lavage fluids were centrifuged at 450g for 10 minutes. Supernatants were used for analysis of cytokines and myeloid-related protein 8 (MRP-8)–MRP-14 complex. Cells were subjected to cytospin staining and flow cytometric analysis. Neutrophil numbers in the peritoneal exudate cells were determined by multiplying the total cell numbers by the percentage of lymphocyte antigen 6 complex, locus G (Ly6G)–positive CD11b⫹ cells in individual mice. Flow cytometric analysis. Peritoneal exudate cells were resuspended in fluorescence-activated cell sorting (FACS) buffer (5% fetal calf serum [FCS] plus 5 mM EDTA in PBS) and incubated with conjugated monoclonal antibodies (mAb). The mAb used were phycoerythrin-conjugated anti–Ly-6G (clone RB6-8C5), fluorescein isothiocyanate (FITC)– conjugated anti-CD11b (clone M1/70), and allophycocyaninconjugated anti-F4/80 (clone BM8) (all from eBioscience). Peritoneal cells (1 ⫻ 106) were incubated with appropriate conjugated antibodies for 30 minutes at 4°C in the dark. Stained cells were subsequently washed twice in FACS buffer and fixed in BD CellFIX solution (BD Biosciences). All data acquisition was performed on a FACSCalibur flow cytometer (BD Biosciences) using CellQuest software (BD Biosciences). Data analysis was performed using FlowJo software (Tree Star). Enzyme-linked immunosorbent assay (ELISA). Cytokine levels in harvested lavage fluid supernatant were analyzed by IL-1␣ ELISA (BioLegend) and IL-1␤ ELISA (eBioscience) according to the manufacturers’ instructions. Concentrations of MRP-8–MRP-14 complex in serum and peritoneal supernatants were determined by ELISA as previously described (28). Isolation of thioglycolate-elicited peritoneal macrophages. Macrophages were isolated from the peritoneal cavity of C57BL/6J mice as described previously (29). Briefly, naive mice were given IP injections of 4% sterile thioglycolate (0.5 ml). After 4 days, peritoneal cells collected by lavage were seeded at 1 ⫻ 106/ml in RPMI 1640 medium supplemented with 10% calf serum and antibiotics for 4 hours to allow the macrophages to adhere to the plates. Nonadherent cells were subsequently removed and adherent macrophages were used for experiments. Isolation of bone marrow–derived mast cells. Bone marrow–derived mast cells were generated from bone marrow of C57BL/6J mice as described previously (30). Briefly, naive mice were killed and intact femurs and tibias were harvested. Sterile RPMI 1640 medium was repeatedly flushed through the bone shaft using a syringe with a 25-G needle. After lysis of red blood cells, cells were washed and cultured at a concentration of 1 ⫻ 106/ml in RPMI 1640 supplemented with 10% FCS, 100 units/ml penicillin, and 100 g/ml streptomycin. Recombinant mouse IL-3 (5 ng/ml; R&D Systems) was added weekly to the cultures. Nonadherent cells were transferred to fresh medium at least once per week. Cells were used after 8 weeks of culture, when a mast cell purity of ⬎95% was achieved as assessed by toluidine blue staining and FACS analysis of CD117 (c-Kit) expression using FITC-conjugated anti-CD117 mAb (clone 2B8; eBioscience). NARAYAN ET AL Depletion of mast cells. Treatment with compound 48/80 (Sigma) was based on slight modifications to a previously reported protocol (31). Briefly, mice were treated IP with compound 48/80 (2 daily injections of 10 g) for 3 days before crystal administration. Control mice received sterile PBS. Mast cell depletion was confirmed by identification of toluidine blue–stained cells in the peritoneal fluid, following which mice were immediately challenged with an IP dose of 1 mg of OCP crystals. Depletion of resident macrophages. Clodronate liposomes were kindly provided by Dr. Nico van Rooijen (VU University Medical Center, Amsterdam, The Netherlands) and were prepared as previously described (32). Mice were given an IP injection of 200 l of clodronate liposomes. Control mice received liposomes containing PBS. Three days later, macrophage depletion in liposome-treated mice was confirmed by flow cytometry, following which mice were immediately challenged with an IP dose of 1 mg of OCP crystals. Peritoneal cell viability. Cell viability was assessed by flow cytometric analysis using the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences) according to the manufacturer’s instructions. Briefly, 6 hours after IP injection of 1 mg of OCP crystals, peritoneal cells were recovered and stained with FITC-conjugated annexin V and propidium iodide (PI). Viable, early apoptotic, and late apoptotic and/or necrotic cells were identified as annexin V negative/PI negative, annexin V positive/PI negative, and annexin V positive/PI positive, respectively. Statistical analysis. All values are expressed as the mean ⫾ SEM. Variation between data sets was evaluated using Student’s t-test or one-way analysis of variance where appropriate. P values less than 0.05 were considered significant. Data were analyzed using GraphPad Prism software. RESULTS OCP crystal stimulation induces acute inflammation. To study the inflammatory response to OCP crystals in vivo, we used an established model of crystalinduced neutrophil infiltration into the peritoneal cavity. In previous experiments we determined that IP administration of OCP crystals induces a dose-dependent accumulation of neutrophils at the site of crystal deposition, with a plateau effect observed between 0.5 mg and 1 mg (data not shown). Therefore, for subsequent in vivo experiments, we used a dose of 1 mg of OCP crystals/mouse. MSU crystals injected at the same dose were used as a positive control. We initially assessed the in vivo kinetics of neutrophils and macrophages during OCP crystal–induced peritonitis. Compared with baseline levels (PBS-injected negative controls), absolute numbers of neutrophils (Ly-6G⫹CD11b⫹) in the peritoneal lavage fluid increased gradually following IP injection of OCP crystals, reaching a plateau by 6 hours (Figure 1A). In contrast, absolute numbers of macro- OCP CRYSTALS ARE PROINFLAMMATORY IN VIVO 425 Figure 1. Octacalcium phosphate (OCP) crystals induce peritoneal inflammation. Naive C57BL/6J mice were injected intraperitoneally (IP) with 1 mg of OCP crystals. Mice injected with phosphate buffered saline (PBS) were used as negative controls. Injected mice were killed 3, 6, 9, 12, or 24 hours following OCP crystal administration. A and B, Neutrophils (A) and macrophages (B) in peritoneal lavage fluid were quantified by flow cytometry. Values are the mean ⫾ SEM from at least 5 mice per group. ⴱⴱⴱ ⫽ P ⬍ 0.001 versus baseline. C, Naive C57BL/6J mice were injected IP with 1 mg of either OCP crystals or monosodium urate monohydrate (MSU) crystals. Mice injected with PBS were used as negative controls. Six hours following crystal administration, neutrophil recruitment into the peritoneal cavity was quantified by flow cytometry. D and E, Levels of interleukin-1␣ (IL-1␣) (D) and IL-1␤ (E) in the peritoneal lavage fluid were assessed by enzyme-linked immunosorbent assay (ELISA). F and G, Levels of myeloid-related protein 8 (MRP-8)–MRP-14 complex in the peritoneal lavage fluid (F) or in serum (G) were assessed by ELISA. Values are the mean ⫾ SEM from at least 6 mice per group. ⴱ ⫽ P ⬍ 0.05; ⴱⴱ ⫽ P ⬍ 0.01; ⴱⴱⴱ ⫽ P ⬍ 0.001. Ly-6G ⫽ lymphocyte antigen 6 complex, locus G. phages (Ly-6G–CD11b⫹) decreased substantially from baseline values 3 hours after IP injection of OCP crystals (Figure 1B). After 6 hours, the numbers of macrophages within the peritoneal lavage fluid began to increase slowly, although this increase was not statistically significant. Since 6 hours after OCP crystal administration appears to be a good time point for showing the acute reaction of both neutrophils and macrophages in response to OCP crystal injection into the peritoneal cavity, the 6-hour time point was used as the end point for peritoneal lavage fluid harvest for subsequent in vivo experiments. We next compared the inflammatory response to OCP and MSU crystals in vivo. Upon IP administration, OCP crystals were able to induce Ly-6G⫹CD11b⫹ neutrophil influx at levels comparable with those achieved following injection of MSU crystals (Figure 1C). As expected, the absolute numbers of peritoneal Ly-6G–CD11b⫹ macrophages decreased significantly after crystal injection (mean ⫾ SEM 706,000 ⫾ 128,000 in PBS-injected mice, 396,000 ⫾ 230,000 in OCP crystal– injected mice, and 121,000 ⫾ 38,000 in MSU crystal– injected mice) (data not shown). The recruitment of neutrophils was associated with elevated levels of both IL-1␣ and IL-1␤ in the peritoneal lavage fluid of crystalinjected mice compared with levels in PBS-injected controls (Figures 1D and E, respectively). These results also correlated with a significant increase in the level of MRP-8–MRP-14 complex, which is considered a reliable marker of inflammation (33,34), both in peritoneal lavage fluid (Figure 1F) and in serum samples (Figure 1G). Taken together, these observations demonstrate 426 NARAYAN ET AL Figure 2. IL-1 blockade or deficiency prevents OCP crystal–induced inflammation. A, Naive mice or mice pretreated with anti–IL-1␤ monoclonal antibodies or anakinra were administrated an IP dose of 1 mg of either OCP crystals or MSU crystals. Six hours after crystal injection, mice were killed and neutrophil accumulation in the peritoneal cavity was assessed by flow cytometry. Values are the mean ⫾ SEM from at least 10 mice per group. B–D, Wild-type, IL-1␣–/–, and IL-1␤–/– mice were injected IP with 1 mg OCP crystals. Six hours after crystal injection, neutrophil accumulation in the peritoneal cavity was quantified by flow cytometry (B), and levels of IL-1␣ (C) and IL-1␤ (D) in the peritoneal lavage fluid were assessed by ELISA. Values are the mean ⫾ SEM from at least 4 mice per group.ⴱ ⫽ P ⬍ 0.05; ⴱⴱ ⫽ P ⬍ 0.01; ⴱⴱⴱ ⫽ P ⬍ 0.001. See Figure 1 for definitions. that in vivo OCP crystal stimulation is able to induce strong inflammation both locally and systemically. Blockade or deficiency of IL-1 strongly reduces OCP crystal–induced inflammation. We next assessed whether OCP crystal–triggered inflammation acts via IL-1–dependent pathways. Naive mice were treated with anakinra (recombinant IL-1Ra), which binds tightly to IL-1R type I, blocking the activity of either IL-1␣ or IL-1␤ (35). Anakinra-treated mice displayed a significant reduction in both OCP crystal– and MSU crystal– induced neutrophil recruitment (Figure 2A). Decreased OCP crystal– and MSU crystal–induced neutrophil infiltration into the peritoneal cavity was also observed in mice treated with IL-1␤–neutralizing antibodies. Collectively, these results demonstrate that IL-1R activation and IL-1 production play essential roles in OCP crystal– triggered inflammation. Since we showed that both IL-1␣ and IL-1␤ levels were increased in peritoneal lavage fluid samples from crystal-injected mice (Figures 1D and E, respectively) and that anti–IL-1␤ antibodies and recombinant IL-1Ra were efficient in blocking neutrophil recruitment, we next assessed the relative contributions of IL-1␣ and IL-1␤ in OCP crystal–induced inflammation. Mice defi- cient in either IL-1␣ or IL-1␤ were injected with OCP crystals, and peritoneal neutrophil recruitment was assessed 6 hours following crystal administration (Figure 2B). In the absence of IL-1␣, there was a striking and significant decrease in neutrophil recruitment upon OCP crystal injection. A similar decrease was observed in IL-1␤–deficient mice, although this was not statistically significant. As expected, IL-1␣–/– and IL-1␤–/– mice indeed did not produce IL-1␣ and IL-1␤, respectively, after OCP crystal administration, and the absence of one of these cytokines affected the production of the other (Figures 2C and D). These results indicate that IL-1␣ and IL-1␤ as independent cytokines are important in crystal-induced neutrophil recruitment. However, these 2 soluble factors when functioning separately may not be solely responsible for facilitating crystal-induced neutrophil accumulation in the peritoneal cavity. OCP crystal–stimulated inflammation is independent of ASC and NLRP3. Since we have shown that IL-1␤ plays a prominent role in OCP crystal–induced inflammation, we next investigated the contribution of the inflammasome, the multiprotein complex able to convert proIL-1␤ into biologically active IL-1␤, to OCP OCP CRYSTALS ARE PROINFLAMMATORY IN VIVO 427 Figure 3. ASC or NLRP3 deficiency does not affect OCP crystal–induced inflammation. A, Wild-type (WT), ASC–/–, and NLRP3–/– mice were injected IP with 1 mg of OCP crystals. Six hours following crystal challenge, neutrophil accumulation in the peritoneal cavity was quantified by flow cytometry. As expected, OCP crystal injection induced a significant increase in neutrophil recruitment compared with that in PBS-injected controls, and similar numbers of neutrophils were recruited in ASC–/– mice, NLRP3–/– mice, and WT mice. B and C, Levels of MRP-8–MRP-14 complex (B) and IL-1␤ (C) in the peritoneal lavage fluid were assessed by ELISA. D, WT, ASC–/–, and NLRP3–/– mice were treated with 200 g of anakinra 30 minutes prior to injection with 1 mg OCP crystals. Six hours after crystal injection, neutrophil recruitment into the peritoneal cavity was quantified by flow cytometry. Anakinra significantly reduced neutrophil recruitment in all mouse strains. Values are the mean ⫾ SEM from at least 10 mice per group. ⴱ ⫽ P ⬍ 0.05; ⴱⴱ ⫽ P ⬍ 0.01; ⴱⴱⴱ ⫽ P ⬍ 0.001. See Figure 1 for other definitions. crystal–induced inflammation. We investigated the roles of the adaptor protein ASC and of the inflammasome sensor NLRP3. ASC–/– and NLRP3–/– mice were injected IP with OCP crystals. Compared with WT mice, these deficient mice did not show any alteration in their inflammatory response, as demonstrated by similar numbers of neutrophils in the peritoneal cavity (Figure 3A) along with comparable levels of MRP-8–MRP-14 complex (Figure 3B) and IL-1␤ (Figure 3C) in the peritoneal lavage fluid. In addition, anakinra had similar inhibitory effects in WT, ASC–/–, and NLRP3–/– mice (Figure 3D). Taken together, these results indicate that these OCP crystal–associated inflammatory responses are independent of the classic NLRP3 inflammasome and suggest that other inflammasomes may play a role, or, alternatively, that OCP crystal–induced inflammation involves a caspase 1–independent IL-1␤–processing mechanism. In this context we have tested the involvement of neutrophil elastase, a proteinase able to convert proIL-1␤ into biologically active IL-1␤ (13,36). Indeed, when mice were treated prophylactically with AAPV, an inhibitor of neutrophil elastase, we found a nonsignifi- cant trend toward a decrease (a 30% reduction) in both neutrophil recruitment and IL-1␤ levels in peritoneal fluid (data not shown). Mast cell depletion does not attenuate OCP crystal–induced inflammation. We first tested whether mast cells were able to respond to OCP crystals by releasing IL-1␣ and IL-1␤, thereby contributing to the onset of OCP crystal–induced inflammation. We therefore generated bone marrow–derived mast cells of ⬎95% purity. OCP crystal stimulation of mast cells, either previously primed with lipopolysaccharide (LPS) or not, resulted in a massive release of IL-1␣ and IL-1␤ into the supernatant (Figures 4A and B, respectively). To study in vivo the effect of this OCP crystal–associated induction of IL-1 by mast cells, we next induced OCP crystal–mediated peritonitis in mast cell–depleted mice. Mice locally injected with compound 48/80 had ⬎95% depletion of peritoneal mast cells (Figure 4C). We observed comparable levels of neutrophil recruitment between OCP crystal–injected mice pretreated with compound 48/80 and those pretreated with PBS (Figure 4D). These results suggest that resident mast cells do not 428 NARAYAN ET AL Figure 4. Mast cells are not required for OCP crystal–induced inflammation. A and B, Bone marrow–derived mast cells from wild-type mice were stimulated with 500 g/ml of OCP crystals in vitro with or without prior priming with lipopolysaccharide (LPS). Six hours following stimulation, culture supernatants were harvested and analyzed for secretion of IL-1␣ (A) and IL-1␤ (B). Values are the mean ⫾ SEM of triplicate cultures. C and D, Mice received 10 g of compound 48/80 in 0.5 ml PBS IP 72, 48, and 24 hours before OCP crystal administration. Control mice were similarly treated with PBS alone. C, Before crystal injection, mast cells from peritoneal fluid of compound 48/80–injected mice or PBS-injected mice were stained with toluidine blue and counted. Values are the mean ⫾ SEM from 3 mice per group. D, Neutrophil accumulation was quantified 6 hours after crystal injection. Values are the mean ⫾ SEM from 5 mice per group. The difference in neutrophil recruitment between non–mast cell–depleted and mast cell–depleted mice was not significant. ⴱ ⫽ P ⬍ 0.05; ⴱⴱⴱ ⫽ P ⬍ 0.001. See Figure 1 for other definitions. play an important role in the cellular response induced following crystal administration. Macrophage depletion abolishes OCP crystal– induced inflammation. Peritoneal macrophages could also be likely candidates that respond to OCP crystals by releasing IL-1␣ and IL-1␤ and thereby triggering OCP crystal–induced inflammation. When purified peritoneal macrophages that were previously primed with LPS (37) were stimulated with OCP crystals, cells were able to release both IL-1␣ and IL-1␤ in supernatants (Figures 5A and B). We next analyzed the inflammatory response to OCP crystals in mice depleted of resident macrophages by pretreatment with clodronate liposomes (32). An IP injection of clodronate liposomes 3 days prior to peritoneal lavage fluid harvest resulted in ⬎90% depletion of resident macrophages (Figure 5C). Control mice received liposomes containing PBS. We found that macrophage-depleted mice were not able to recruit neutrophils upon IP administration of OCP crystals, whereas in non– macrophage-depleted mice the vast majority of peritoneal cells were neutrophils (Figures 5C and D). As expected, IL-1␤ levels in peritoneal lavage fluid were significantly decreased in mice pretreated with clodronate liposomes (Figure 5E). OCP crystals induce apoptosis/necrosis of peritoneal cells in vivo. We have found that OCP crystals have a deleterious effect on the viability of murine bone marrow–derived macrophages cultured in vitro (⬃50% cell death after 6-hour incubation with 500 g/ml OCP crystals) (37). In order to assess the relevance of such a phenomenon following in vivo OCP crystal administration, peritoneal cells were costained with annexin V and PI. We found that ⬃50% of cells recovered 6 hours after IP injection of OCP crystals were dead (either in late apoptosis or in necrosis). In contrast, the vast majority of cells from PBS-injected mice were viable (Figures 6A and B). DISCUSSION OCP crystals, members of the BCP crystal family, elicit joint as well as periarticular inflammation and may have a pathogenic role in OA. The mechanisms of inflammation due to BCP crystals have not been extensively studied. Here we report that in the murine peri- OCP CRYSTALS ARE PROINFLAMMATORY IN VIVO 429 Figure 5. Resident macrophages are crucial cells for OCP crystal–induced inflammation. A and B, Bone marrow–derived macrophages from wild-type mice were stimulated with 500 g/ml of OCP crystals in vitro with or without prior priming with lipopolysaccharide (LPS). Six hours following stimulation, culture supernatants were harvested and analyzed for secretion of IL-1␣ (A) and IL-1␤ (B). C–E, Mice were treated IP with 200 l of clodronate liposomes. Control mice received liposomes containing PBS. Three days later, mice were challenged with an IP injection of 1 mg of OCP crystals. Peritoneal lavage fluid was collected 6 hours after crystal stimulation, and the recruitment of peritoneal neutrophils was assessed by flow cytometry (C and D). In C, neutrophils (Ly-6G⫹F4/80–) are represented as R2; macrophages (Ly-6G⫺F4/80⫹) are represented as R3. Top, Mice treated with PBS containing liposomes only; middle, OCP crystal–injected mice previously pretreated with PBS containing liposomes; bottom, OCP crystal–injected mice previously pretreated with clodronate liposomes. IL-1␤ levels in the peritoneal lavage fluid from macrophage-depleted and non–macrophage-depleted mice injected with crystals were assessed by ELISA (E). Values in A, B, D, and E are the mean ⫾ SEM from 5 mice per group. Plots in C are representative of 3 mice per group. ⴱ ⫽ P ⬍ 0.05; ⴱⴱⴱ ⫽ P ⬍ 0.001. PE ⫽ phycoerythrin; APC ⫽ allophycocyanin (see Figure 1 for other definitions). Color figure can be viewed in the online issue, which is available at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1529-0131. tonitis model, OCP crystals cause a strong recruitment of neutrophils that is comparable with levels achieved with MSU crystals, whose proinflammatory properties have been well documented (20,38,39). These findings prompted us to investigate the molecular mechanisms responsible for neutrophil accumulation in an experimental model of OCP crystal–induced peritonitis. The recent emergence of the inflammasome as a caspase 1 activator and its role in hereditary autoinflammatory syndromes and in gout raises the question of whether this complex is equally relevant in IL-1␤ pro- duction in BCP crystal– and, especially, OCP crystal– induced inflammatory situations. To address the role of the inflammasome in OCP crystal–induced pathogenesis, we assessed the effect of genetic deletion of different elements of the inflammasome on neutrophil recruitment into the peritoneal cavity upon OCP crystal administration. We found no effect of either ASC or NLRP3 deficiency on neutrophil recruitment, and similar amounts of MRP were detected in the peritoneal cavity. The absence of an obvious influence of NLRP3 and ASC on OCP crystal–induced inflammation un- 430 NARAYAN ET AL Figure 6. OCP crystals induce apoptosis/necrosis of peritoneal cells in vivo. A and B, Mice were injected with PBS as a control or with 1 mg of OCP crystals. After 6 hours, cells were recovered from peritoneal lavage fluid and analyzed for viability by flow cytometry using annexin V and propidium iodide (PI) staining. Viable, early apoptotic, and late apoptotic and/or necrotic cells were identified as annexin V negative/PI negative, annexin V positive/PI negative, and annexin V positive/PI positive, respectively. Values are the mean ⫾ SEM from 4 mice per group. ⴱⴱⴱ ⫽ P ⬍ 0.001. C, Model of OCP crystal–induced peritoneal inflammation. The major event triggering OCP crystal–induced peritoneal inflammation is mediated by OCP crystal–induced cell death. IL-1␣ is released from necrotic cells. IL-1␣ and IL-1␤ bind to IL-1 receptor type I (IL-1RI) on target cells. IL-1RI activation subsequently induces the production of CXCL1 by mesothelial cells and fibroblasts. CXCL1 in turn facilitates neutrophil recruitment, which can also be mediated by MRP and IL-8. ProIL-1␤ can also be released from dead cells. Proteinases derived from neutrophils (such as proteinase 3, elastase, and cathepsin G) or mast cells (such as chymase and cathepsin G) can activate proIL-1␤ independent of the inflammasome. See Figure 1 for other definitions. equivocally rules out a role for the NLRP3 inflammasome in OCP-induced inflammatory responses in vivo. These findings contrast with observations that MSU crystals (20), as well as inorganic particles such as asbestos fibers, silica particles, and alum crystals, activate the NLRP3 inflammasome to produce IL-1␤ (40–42). The presence of IL-1␤ in the peritoneal exudate and the attenuation of inflammation by IL-1␤ blockade and IL-1␤ deficiency suggest that there is an NLRP3 inflammasome–independent mechanism. We and others have recently demonstrated the existence of inflammasome-independent pathways of IL-1␤ processing in IL-1␤–mediated diseases such as antigen-induced arthritis (43), collagen-induced arthritis (44), K/BxN serum transfer–induced arthritis (14), acute arthritis (15), and experimental models of infections (for review, see ref. 11). In these disease models, enzymes distinct from caspase 1 are able to process proIL-1␤ (discussed in refs. 11 and 16). Neutrophil-, macrophage-, and mast cell–derived serine proteinases such as proteinase 3, elastase, cathepsin G, and chymase have been reported to be able to convert proIL-1␤ into the 21-kd active form (10,12,13). A crucial role of chymase and elastase in proIL-1␤ activation was recently shown in the K/BxN arthritis model using specific proteinase inhibitors (14). Consistent with this latter finding, we tested the effects of AAPV in OCP crystal–induced peritonitis and found a 30% reduction in neutrophil recruitment and peritoneal IL-1␤ (this difference did not reach significance OCP CRYSTALS ARE PROINFLAMMATORY IN VIVO [data not shown]), suggesting that a part of proIL-1␤ processing in OCP crystal–induced peritonitis is due to neutrophil elastase. OCP crystal–induced inflammation in the peritonitis model depends on both IL-1␣ and IL-1␤. The first line of evidence for this was the finding that both IL-1␣ and IL-1␤ were released into the peritoneal exudate after crystal injection. A role for both cytokines was highlighted by the inhibitory effects of individual deficiency of IL-1␣ and IL-1␤. Interestingly, their effects on inflammation seem to be linked, since IL-1␣ deficiency had an effect on peritoneal IL-1␤ levels and vice versa. Such a reciprocal regulation of IL-1␣ over the production of IL-1␤ has been previously reported in another experimental model (26). IL-1␣ is biologically active in its precursor form and can be found on the surface of several cells, particularly monocytes, where it is referred to as membrane IL-1␣ (10). Cleavage of the precursor by calpain, a membrane-associated calcium-activated cysteine proteinase, releases mature IL-1␣. It may also be released from dying cells (45). We demonstrated that OCP crystals induced necrosis of ⬃50% of peritoneal cells, principally represented by infiltrating neutrophils. In this context, it is likely that at least part of the peritoneal fluid IL-1␣ that we measured following OCP crystal injection was released from dying cells. Interestingly, it has been shown that IL-1␣ released from necrotic cells triggers CXCL1/ cytokine-induced neutrophil chemoattractant (KC) secretion and recruitment of neutrophils via IL-1R/ myeloid differentiation factor 88 signaling on neighboring mesothelial cells (46). Therefore, we can anticipate that the inflammatory properties of OCP crystals include their ability to induce cellular necrosis. The subsequent passive release of IL-1␣ from dying cells would in turn facilitate chemokine production (eventually CXCL1) and neutrophil recruitment to the inflamed site. Validation of such a mechanism would require crystal administration in mice with a targeted mutation in CXCL1/KC (47). It has recently been shown that in addition to passive release of danger signals such as uric acid or ATP, necrotic cells drive inflammatory cell infiltration in vivo and induce the production of IL-1␤ in an NLRP3 inflammasome–dependent manner (42,48). Our data do not support the influence of such a mechanism, since neutrophil recruitment and IL-1␤ production were not altered in the absence of the NLRP3 inflammasome. Such a discrepancy could be explained by the dose of necrotic cells required to activate the NLRP3 inflammasome. To trigger a sterile inflammatory response 431 through NLRP3, 107 necrotic cells were injected IP (48). In our experimental setting, cells were progressively dying and we recovered ⬃10-fold fewer necrotic cells 6 hours following crystal administration. Thus, below a certain threshold of necrosis, the NLRP3 inflammasome is probably not activated. Mast cells and resident macrophages have been implicated in MSU crystal–induced peritonitis (36,38), and we investigated the contribution of these cells in OCP crystal–induced peritonitis. Depletion studies using either compound 48/80 or clodronate liposomes showed that only peritoneal macrophage depletion led to a reduction of neutrophil recruitment and IL-1␤ levels, demonstrating that resident macrophages play an essential role in the production of IL-1␤ and in neutrophil recruitment in OCP crystal–induced inflammation, even though in in vitro studies, both cell types secreted IL-1␤ when stimulated with OCP crystals. We recently found that OCP crystals stimulated IL-1␤ secretion in murine bone marrow–derived macrophages and peritoneal macrophages through an NLRP3 inflammasome– dependent pathway in vitro (37). The difference, in terms of NLRP3 dependency, between the in vivo and in vitro results may be due to OCP crystal–induced cell death and the release of non–caspase 1 proteinases already discussed, or to factors that can modulate crystal interactions with cells, such as protein coating of crystals in vivo (24,25). An interesting observation during crystal-induced peritonitis was that peritoneal macrophages decreased significantly in the peritoneal lavage fluid following IP administration of both OCP and MSU crystals. Similar disappearance of macrophages has been previously observed in harvested peritoneal lavage fluid following IP injection with MSU crystals (49). This disappearance of macrophages shortly following administration of inflammatory stimuli has been termed “macrophage disappearance reaction” and has been highlighted in other models of acute inflammation (50–52). Finally, we observed a significant release of MRP-8–MRP-14 complex (S100A8/A9, calprotectin) during OCP crystal–induced peritonitis, which may further amplify the local inflammatory response. MRPs are secreted by monocytes and neutrophils following cellular activation or necrosis (for review, see ref. 53) and participate in a positive feedback loop of neutrophil recruitment by up-regulating integrin expression and mediating chemotaxis (54,55). Not only peritoneal but also serum levels of MRP-8–MRP-14 complex were increased upon OCP crystal injection. Similar results were reported in a murine air pouch model of MSU 432 NARAYAN ET AL crystal–induced inflammation that was inhibited by antiMRP antibodies (56). This suggests that in BCP crystal– related diseases such as the Milwaukee shoulder syndrome, MRP levels will be increased and might play a role in pathogenesis. In conclusion, we have demonstrated that in an in vivo model, OCP crystals induce inflammation via IL-1␣ and IL-1␤, independent of the NLRP3 inflammasome, and this process is linked to cell death induced by crystals (Figure 6C). Furthermore, our data highlight that macrophages play a crucial role in this inflammatory process but mast cells do not. These mechanisms can account for the acute inflammatory reaction seen in acute periarthritis and arthritis due to OCP crystals. In OA, in which BCP crystals are found in the cartilage as well as in the joint fluid, these mechanisms do not seem to predominate, since inflammation is not a prominent feature. These results have implications in the search for effective therapies for BCP crystal–associated diseases. 5. 6. 7. 8. 9. 10. 11. 12. ACKNOWLEDGMENTS We are grateful to Dr. Nico van Rooijen (VU University Medical Center, Amsterdam, The Netherlands) for kindly providing us with clodronate liposomes. We thank Dr. Yoichiro Iwakura (University of Tokyo, Tokyo, Japan) for providing us with IL-1␣–/– and IL-1␤–/– mice and Professor Jurg Tschopp (University of Lausanne, Lausanne, Switzerland) for providing us with NLRP3–/– and ASC–/– mice. 13. 14. 15. AUTHOR CONTRIBUTIONS All authors were involved in drafting the article or revising it critically for important intellectual content, and all authors approved the final version to be published. Dr. Busso had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study conception and design. Narayan, Pazar, Ea, Kolly, Bagnoud, Chobaz, Lioté, So, Busso. Acquisition of data. Narayan, Pazar, Ea, Kolly, Bagnoud, Chobaz, Vogl, Holzinger. Analysis and interpretation of data. Narayan, Pazar, Ea, Kolly, Bagnoud, Chobaz, Lioté, Vogl, Holzinger, So, Busso. 16. 17. 18. 19. 20. REFERENCES 1. Nalbant S, Martinez JA, Kitumnuaypong T, Clayburne G, Sieck M, Schumacher HR Jr. Synovial fluid features and their relations to osteoarthritis severity: new findings from sequential studies. Osteoarthritis Cartilage 2003;11:50–4. 2. Fuerst M, Bertrand J, Lammers L, Dreier R, Echtermeyer F, Nitschke Y, et al. Calcification of articular cartilage in human osteoarthritis. Arthritis Rheum 2009;60:2694–703. 3. Fuerst M, Niggemeyer O, Lammers L, Schafer F, Lohmann C, Ruther W. Articular cartilage mineralization in osteoarthritis of the hip. BMC Musculoskelet Disord 2009;10:166. 4. Halverson PB, Cheung HS, McCarty DJ, Garancis J, Mandel N. “Milwaukee shoulder”—association of microspheroids containing 21. 22. 23. hydroxyapatite crystals, active collagenase, and neutral protease with rotator cuff defects. II. Synovial fluid studies. Arthritis Rheum 1981;24:474–83. Ea HK, Liote F. Advances in understanding calcium-containing crystal disease. Curr Opin Rheumatol 2009;21:150–7. McCarthy GM, Cheung HS. Point: hydroxyapatite crystal deposition is intimately involved in the pathogenesis and progression of human osteoarthritis. Curr Rheumatol Rep 2009;11:141–7. Ea HK, Monceau V, Camors E, Cohen-Solal M, Charlemagne D, Liote F. Annexin 5 overexpression increased articular chondrocyte apoptosis induced by basic calcium phosphate crystals. Ann Rheum Dis 2008;67:1617–25. Ea HK, Uzan B, Rey C, Liote F. Octacalcium phosphate crystals directly stimulate expression of inducible nitric oxide synthase through p38 and JNK mitogen-activated protein kinases in articular chondrocytes. Arthritis Res Ther 2005;7:R915–26. Prudhommeaux F, Schiltz C, Liote F, Hina A, Champy R, Bucki B, et al. Variation in the inflammatory properties of basic calcium phosphate crystals according to crystal type. Arthritis Rheum 1996;39:1319–26. Dinarello CA. Immunological and inflammatory functions of the interleukin-1 family. Annu Rev Immunol 2009;27:519–50. Netea MG, Simon A, van de Veerdonk F, Kullberg BJ, van der Meer JW, Joosten LA. IL-1␤ processing in host defense: beyond the inflammasomes. PLoS Pathog 2010;6:e1000661. Mizutani H, Schechter N, Lazarus G, Black RA, Kupper TS. Rapid and specific conversion of precursor interleukin 1␤ (IL-1␤) to an active IL-1 species by human mast cell chymase. J Exp Med 1991;174:821–5. Coeshott C, Ohnemus C, Pilyavskaya A, Ross S, Wieczorek M, Kroona H, et al. Converting enzyme-independent release of tumor necrosis factor ␣ and IL-1␤ from a stimulated human monocytic cell line in the presence of activated neutrophils or purified proteinase 3. Proc Natl Acad Sci U S A 1999;96:6261–6. Guma M, Ronacher L, Liu-Bryan R, Takai S, Karin M, Corr M. Caspase 1–independent activation of interleukin-1␤ in neutrophilpredominant inflammation. Arthritis Rheum 2009;60:3642–50. Joosten LA, Netea MG, Fantuzzi G, Koenders MI, Helsen MM, Sparrer H, et al. Inflammatory arthritis in caspase 1 gene–deficient mice: contribution of proteinase 3 to caspase 1–independent production of bioactive interleukin-1␤. Arthritis Rheum 2009;60: 3651–62. Stehlik C. Multiple interleukin-1␤–converting enzymes contribute to inflammatory arthritis [editorial]. Arthritis Rheum 2009;60: 3524–30. Martinon F, Mayor A, Tschopp J. The inflammasomes: guardians of the body. Annu Rev Immunol 2009;27:229–65. Schroder K, Zhou R, Tschopp J. The NLRP3 inflammasome: a sensor for metabolic danger? Science 2010;327:296–300. Mariathasan S, Monack DM. Inflammasome adaptors and sensors: intracellular regulators of infection and inflammation. Nat Rev Immunol 2007;7:31–40. Martinon F, Petrilli V, Mayor A, Tardivel A, Tschopp J. Goutassociated uric acid crystals activate the NALP3 inflammasome. Nature 2006;440:237–41. Announ N, Palmer G, Guerne PA, Gabay C. Anakinra is a possible alternative in the treatment and prevention of acute attacks of pseudogout in end-stage renal failure. Joint Bone Spine 2009;76:424–6. So A, De Smedt T, Revaz S, Tschopp J. A pilot study of IL-1 inhibition by anakinra in acute gout. Arthritis Res Ther 2007;9: R28. Terkeltaub R, Sundy JS, Schumacher HR, Murphy F, Bookbinder S, Biedermann S, et al. The interleukin 1 inhibitor rilonacept in treatment of chronic gouty arthritis: results of a placebo-controlled, monosequence crossover, non-randomised, single-blind pilot study. Ann Rheum Dis 2009;68:1613–7. OCP CRYSTALS ARE PROINFLAMMATORY IN VIVO 24. Bardin T, Varghese Cherian P, Schumacher HR. Immunoglobulins on the surface of monosodium urate crystals: an immunoelectron microscopic study. J Rheumatol 1984;11:339–41. 25. Ortiz-Bravo E, Sieck MS, Schumacher HR Jr. Changes in the proteins coating monosodium urate crystals during active and subsiding inflammation: immunogold studies of synovial fluid from patients with gout and of fluid obtained using the rat subcutaneous air pouch model. Arthritis Rheum 1993;36:1274–85. 26. Horai R, Asano M, Sudo K, Kanuka H, Suzuki M, Nishihara M, et al. Production of mice deficient in genes for interleukin (IL)-1␣, IL-1␤, IL-1␣/␤, and IL-1 receptor antagonist shows that IL-1␤ is crucial in turpentine-induced fever development and glucocorticoid secretion. J Exp Med 1998;187:1463–75. 27. Mariathasan S, Newton K, Monack DM, Vucic D, French DM, Lee WP, et al. Differential activation of the inflammasome by caspase-1 adaptors ASC and Ipaf. Nature 2004;430:213–8. 28. Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, et al. Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock. Nat Med 2007;13:1042–9. 29. Kreckler LM, Wan TC, Ge ZD, Auchampach JA. Adenosine inhibits tumor necrosis factor-␣ release from mouse peritoneal macrophages via A2A and A2B but not the A3 adenosine receptor. J Pharmacol Exp Ther 2006;317:172–80. 30. Moulin D, Donze O, Talabot-Ayer D, Mezin F, Palmer G, Gabay C. Interleukin (IL)-33 induces the release of pro-inflammatory mediators by mast cells. Cytokine 2007;40:216–25. 31. Getting SJ, Flower RJ, Parente L, de Medicis R, Lussier A, Woliztky BA, et al. Molecular determinants of monosodium urate crystal-induced murine peritonitis: a role for endogenous mast cells and a distinct requirement for endothelial-derived selectins. J Pharmacol Exp Ther 1997;283:123–30. 32. Van Rooijen N, Sanders A, van den Berg TK. Apoptosis of macrophages induced by liposome-mediated intracellular delivery of clodronate and propamidine. J Immunol Methods 1996;193: 93–9. 33. Herndon BL, Abbasi S, Bennett D, Bamberger D. Calciumbinding proteins MRP 8 and 14 in a Staphylococcus aureus infection model: role of therapy, inflammation, and infection persistence. J Lab Clin Med 2003;141:110–20. 34. Kunimi K, Maegawa M, Kamada M, Yamamoto S, Yasui T, Matsuzaki T, et al. Myeloid-related protein-8/14 is associated with proinflammatory cytokines in cervical mucus. J Reprod Immunol 2006;71:3–11. 35. Cohen SB. The use of anakinra, an interleukin-1 receptor antagonist, in the treatment of rheumatoid arthritis. Rheum Dis Clin North Am 2004;30:365–80. 36. Hazuda DJ, Strickler J, Kueppers F, Simon PL, Young PR. Processing of precursor interleukin 1␤ and inflammatory disease. J Biol Chem 1990;265:6318–22. 37. Pazar B, Ea HK, Narayan S, Kelly L, Bagnoud N, Chobaz V, et al. Basic calcium phosphate crystals induce monocyte/macrophage IL-1␤ secretion through the NLRP3-inflammasome in vitro. J Immunol. In press. 38. Jaramillo M, Godbout M, Naccache PH, Olivier M. Signaling events involved in macrophage chemokine expression in response to monosodium urate crystals. J Biol Chem 2004;279:52797–805. 39. Schumacher HR. Crystal-induced arthritis: an overview. Am J Med 1996;100:46–52S. 40. Dostert C, Petrilli V, van Bruggen R, Steele C, Mossman BT, 433 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. Tschopp J. Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science 2008;320:674–7. Hornung V, Bauernfeind F, Halle A, Samstad EO, Kono H, Rock KL, et al. Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization. Nat Immunol 2008;9:847–56. Li H, Ambade A, Re F. Cutting edge: necrosis activates the NLRP3 inflammasome. J Immunol 2009;183:1528–32. Kolly L, Karababa M, Joosten LA, Narayan S, Salvi R, Petrilli V, et al. Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF. J Immunol 2009; 183:4003–12. Ippagunta SK, Brand DD, Luo J, Boyd KL, Calabrese C, Stienstra R, et al. Inflammasome-independent role of apoptosis-associated speck-like protein containing a CARD (ASC) in T cell priming is critical for collagen-induced arthritis. J Biol Chem 2010;285: 12454–62. Chen CJ, Kono H, Golenbock D, Reed G, Akira S, Rock KL. Identification of a key pathway required for the sterile inflammatory response triggered by dying cells. Nat Med 2007;13:851–6. Eigenbrod T, Park JH, Harder J, Iwakura Y, Nunez G. Cutting edge: critical role for mesothelial cells in necrosis-induced inflammation through the recognition of IL-1␣ released from dying cells. J Immunol 2008;181:8194–8. Boisvert WA, Rose DM, Johnson KA, Fuentes ME, Lira SA, Curtiss LK, et al. Up-regulated expression of the CXCR2 ligand KC/GRO-␣ in atherosclerotic lesions plays a central role in macrophage accumulation and lesion progression. Am J Pathol 2006;168:1385–95. Iyer SS, Pulskens WP, Sadler JJ, Butter LM, Teske GJ, Ulland TK, et al. Necrotic cells trigger a sterile inflammatory response through the Nlrp3 inflammasome. Proc Natl Acad Sci U S A 2009;106: 20388–93. Martin WJ, Walton M, Harper J. Resident macrophages initiating and driving inflammation in a monosodium urate monohydrate crystal–induced murine peritoneal model of acute gout. Arthritis Rheum 2009;60:281–9. Haskill S, Becker S. Disappearance and reappearance of resident macrophages: importance in C. parvum-induced tumoricidal activity. Cell Immunol 1985;90:179–89. Melnicoff MJ, Horan PK, Morahan PS. Kinetics of changes in peritoneal cell populations following acute inflammation. Cell Immunol 1989;118:178–91. Nelson DS, Boyden SV. The loss of macrophages from peritoneal exudates following the injection of antigens into guinea-pigs with delayed-type hypersensitivity. Immunology 1963;6:264–75. Perera C, McNeil HP, Geczy CL. S100 calgranulins in inflammatory arthritis. Immunol Cell Biol 2010;88:41–9. Lackmann M, Rajasekariah P, Iismaa SE, Jones G, Cornish CJ, Hu S, et al. Identification of a chemotactic domain of the pro-inflammatory S100 protein CP-10. J Immunol 1993;150: 2981–91. Ryckman C, Vandal K, Rouleau P, Talbot M, Tessier PA. Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion. J Immunol 2003;170:3233–42. Ryckman C, McColl SR, Vandal K, de Medicis R, Lussier A, Poubelle PE, et al. Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air-pouch model of acute gouty arthritis. Arthritis Rheum 2003;48:2310–20.