Abstracts © 2017 Wolters Kluwer S63 325.5 325.6 Results of Kidney Transplant From Donors With Sepsis-Induced Acute Kidney Injury Knowledge & Attitude of Health Staff on Deceased Donor Organ Donation & Transplantation in a Sri Lankan Tertiary Care Setting Agnieszka Wieczorek1, Małgorzata Lipińska-Gediga1, Agnieszka Siebert1, Dariusz Patrzałek2 1 Anesthesia and Intensive Care, 4th Military Hospital, Wrocław, Poland; 2 Physiotherapy, Medical University Wroclaw, Wrocław, Poland. Prasad Herath1, M.H.P. Godakandage1, W.A.N.N. Peries1, L.P. Dilrukshi1, T.D.S. Gunasekera1, A.R.S. Fernando1, N.H.E. Hareendra De Silva1, M.N. Danansuriya2, K.K.C. De Silva1, Ruwan Dissanayake1 1 Transplant Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka; 2 Public Health Complex, Ministry of Health, Colombo, Sri Lanka. Introduction: Acute kidney injury (AKI) is a syndrome comprising multiple clinical conditions and the most common cause of AKI in critically ill patients is sepsis. The pathophysiology of sepsis-induced AKI is complex and multifactorial. The functional consequences during sepsis-induced AKI are dramatic, the histological changes are moderate and do not entirely explain the clinical status. Resolved septicemia is a relative contraindication to organ procurement. Methods: Two young patients with brain injury were hospitalized on ICU and both developed sepsis due to aspiration pneumonia. These patients were around 30 years old and had no medical history before this hospitalization. The antibiotic treatment was in accordance with microbiological results of BAL and blood cultures. Both patients developed sepsis-induced AKI and were treated with CRRT. Bood microbiological test were negative in third day of antibiotic therapy. After diagnosis of brain death they were classified as marginal donors with diagnosis of resolved septicemia. We decided to transplant the organs after the optimistic histological results of biopsy taken from retrieved kidneys. Results: The histological exam showed focal and mild acute tubular necrosis without inflammation and four kidneys were transplant. None of the organ recipient developed any inflammatory complications and their transplant kidney function one year after transplant procedure remains stabile. Conclusion: The pathomechanism of sepsis related AKI is complex and patient with sepsis related AKI may become a kidney donor in particular conditions. References: 1. Zarbock A, Gomez H, Kellum JA. Sepsis-induced acute kidney injury revisited: pathophysiology, prevention and future therapies. Curr Opin Crit Care 2014, 20:588-595. 2. Ronco C, Kellum J, Bellomo R, House AA. Potential Interventions in Sepsis-Related Acute Kidney Injury. Clin J Am Soc Nephro 3: 531-544, 2008. Introduction: Deceased donor organ donation and transplantation in Sri Lanka is still in its early stages. Lack of organs is a significant problem at present. Further, availability of deceased donors lags behind those of live. One of the presumed reasons for this is poor knowledge and attitude among the health care staff and general public. The present study aimed to assess the knowledge and attitude of medical and nursing staff in surgical and medical intensive care units (ICU) in the National Hospital of Sri Lanka (NHSL) on deceased organ donation. Method: A pre-tested, self-administered questionnaire on knowledge and attitude was used. Sample contained doctors and nursing officers of the ICUs of NHSL where potential donors are common. A percentage score was calculated with regard to knowledge and attitude. Ethical approval was obtained from the ethical committee of National Hospital of Sri Lanka. Results: Sample contained 167 subjects; 110 (65.9%) nursing officers and 57 (34.1%) doctors. Majority were females (87.4%) and were in the 31-40-year age group (90, 53.9%). Of the sample, 110 (65.9%) had studied up to diploma, 44 (26.3%) up to basic degree and 13 (7.8%) were undergoing postgraduate training. Majority were Buddhists (102, 61.1%) followed by Muslims (20,12%) and Catholics (19,11.4%). About 40% (68) had ICU work experience of 1-5 years with the mean experience being 6.9 years (range 1-24) and 74.3% (124) were involved in the deceased organ donation process either in preparation or coordination. Of the sample, 87.4% (146) had a knowledge score of more than 50% with 4 (2.3%) achieving 100%. Nearly 75% (n, 127) acknowledged that they have adequate knowledge to address the public, however only 46.7% (78) were aware about the related legal procedures on deceased donor organ donation and transplantation in Sri Lanka. Majority (153,91.6%) possessed a positive attitude on organ donation while admitting the need for better awareness. There were no statistically significant associations between the age, designation, sex, level of education, religion or work experience with regard to knowledge and attitude (p>0.05). Conclusion: There is a satisfactory knowledge and positive attitude among the medical and nursing staff in ICUs of NHSL on deceased organ donation and transplantation. However, knowledge on certain aspects need to be further improved specially on procedural and legal aspects, which may increase deceased organ donations in Sri Lanka. Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.