close

Вход

Забыли?

вход по аккаунту

?

1873-3468.12886

код для вставкиСкачать
Article type
: Research Letter
Accepted Article
Phosphorylation of Aspergillus fumigatus PkaR Impacts Growth and Cell
Wall Integrity Through Novel Mechanisms
E. Keats Shwaba, Praveen R. Juvvadia, Greg Waittb, Erik J. Soderblomb, M. Arthur
Moseleyb, Nathan I. Nicelyc and William J. Steinbacha,d*
a
Department of Pediatrics, Division of Pediatric Infectious Diseases, Duke University
Medical Center, Durham NC, USA
b
Duke Proteomics and Metabolomics Core Facility, Center for Genomic and Computational
Biology, Duke University, Durham NC, USA
c
Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA
d
Department of Molecular Genetics and Microbiology, Duke University Medical Center,
Durham NC, USA
Running Head: Phosphorylation of Aspergillus fumigatus PkaR
* Address for correspondence:
William J. Steinbach,
Department of Pediatrics, Division of Pediatric Infectious Diseases,
Duke University Medical Center, Durham, NC 27710, USA
bill.steinbach@duke.edu
ABSTRACT
PKA signaling is essential for growth and virulence of the fungal pathogen Aspergillus
fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi.
Employing LC-MS/MS, we identified novel phosphorylation sites on the regulatory subunit
PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the
This article has been accepted for publication and undergone full peer review but has not
been through the copyediting, typesetting, pagination and proofreading process, which may
lead to differences between this version and the Version of Record. Please cite this article as
doi: 10.1002/1873-3468.12886
This article is protected by copyright. All rights reserved.
importance of two phosphorylation clusters for hyphal growth and cell wall stress response.
Accepted Article
We also identified key differences in the regulation of PKA subcellular localization in A.
fumigatus compared to other species. This is the first analysis of the phosphoregulation of a
PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences
between PKA regulation in filamentous fungi compared to mammals and yeast species,
suggesting divergent targeting opportunities.
KEYWORDS
Aspergillus fumigatus, Protein kinase A, Phosphorylation, Signaling pathway regulation, Cell
wall integrity, Echinocandin
ABBREVIATIONS
PKA, protein kinase A
CSP, caspofungin
LC-MS/MS, Liquid chromatography-tandem mass spectroscopy
INTRODUCTION
The filamentous fungal pathogen Aspergillus fumigatus is a major infectious cause of
mortality in immunocompromised patients [1-3]. In this fungus, the cAMP signaling pathway
is responsible for regulating diverse processes and has been shown to be essential for
virulence in an immunosuppressed murine model of invasive aspergillosis [4-6]. The master
regulator of the cAMP pathway is protein kinase A (PKA), activated by release of the PKA
catalytic (C) subunit from an inhibitory complex with the regulatory (R) subunit following
binding of cAMP to the latter component. In contrast to the highly conserved structure of the
PKA C subunit, marked diversity is observed among the R subunits of various species,
This article is protected by copyright. All rights reserved.
allowing for refinement of regulatory function and consequent modulation of PKA activity to
Accepted Article
suit the specific requirements of different groups of organisms [7].
In mammals, multiple R subunits direct the subcellular localization of PKA in various tissues
through interaction with A-kinase Anchor Proteins (AKAPs) [8]. While AKAP homologues
have not been identified in fungi, PKA R subunits have been shown to control PKA
localization in a number of fungal species, primarily via shuttling of the C subunit between
the cytoplasm and nucleus, though the specific mechanisms are not well understood [9]. In
the ascomycete model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe,
localization of both the PKA C and R subunits is carbon source-dependent. In these species,
both subunits are primarily observed in the nucleus during rapid log phase growth in the
presence of abundant glucose, whereas glucose starvation during stationary phase growth or
growth on an alternate carbon source results in migration of both subunits into the cytoplasm
[10, 11]. In contrast, the PKA C subunit of the pathogenic yeast Candida albicans was found
to be primarily localized to the nucleus during stationary phase growth [12], indicating
differences in R subunit function even among closely related species. In all three yeasts, C
subunit localization appears to be directed by the R subunit under non-cAMP-inducing
conditions (i.e. low glucose), when the inactive PKA holoenzyme tetramer would be
expected to assemble [10-12].
While cAMP-mediated activation of PKA occurs through a highly conserved mechanism,
other more specialized avenues for regulating PKA activity have also been identified. In
particular, phosphorylation of both C and R subunits is known to play an important
regulatory role in a number of species. In addition to activation through conserved C subunit
phosphorylation as in mammals and other fungi, we previously reported a unique mechanism
This article is protected by copyright. All rights reserved.
of PKA inhibition in the pathogenic filamentous fungus A. fumigatus involving
Accepted Article
phosphorylation of the C subunit substrate-binding cleft [13]. We also showed that deletion
of pkaC1, encoding the major PKA C subunit, resulted in hypersensitivity to the cell walltargeting antifungal agent caspofungin, implicating a role for the PKA-signaling pathway in
the cell wall-stress response. Genomic analyses undertaken to identify genes involved in
caspofungin susceptibility in budding yeast have revealed the importance of the PKCmediated cell wall integrity pathway in addition to other genes involved directly in cell wall
and cell membrane biosynthesis [14]. Furthermore, PKC inhibition was found to result in
increased susceptibility to cell wall inhibitors in clinical Aspergillus species isolates [14].
Regulation through R subunit phosphorylation has been primarily studied in S. cerevisiae,
wherein phosphorylation of N-terminal serine clusters in this protein has been shown to
mediate carbon source-dependent migration from the nucleus into the cytoplasm [15-19]. A
mutant mimicking constitutive phosphorylation in these clusters displayed increased
cytoplasmic localization in the presence of glucose, while a phosphorylation-deficient mutant
localized to the nucleus during growth with ethanol as the sole carbon source [18].
Phosphorylation of a specific site within this N-terminal region was found to have a
suppressive effect on PKA function, suggesting that this modification enhances the inhibitory
capacity of the S. cerevisiae R subunit [16]. Both mammalian type II and S. cerevisiae R
subunits possess an autoinhibitory PKA target site identified as phosphorylated in vivo [2022]. Phosphorylation of this site in S. cerevisiae was shown to occur via PKA C subunit in
vitro, and mutation to alanine resulted in greater binding affinity between the C and R
subunits, leading to a tenfold increase in inhibition efficiency, while the phosphomimetic
mutation to glutamate or aspartate resulted in reduced inhibition [20]. In vivo,
phosphorylation of the autoinhibitory site was found to increase during stationary phase
This article is protected by copyright. All rights reserved.
growth and the alanine substitution strain exhibited a markedly slower doubling time relative
Accepted Article
to the wild-type strain, as well as a transition to stationary phase at a lower optical density
[20].
No experimental observations are available regarding specialized regulatory mechanisms of
the PKA R subunit in filamentous fungi, including model organisms or fungal pathogens. In
A. fumigatus, deletion of the single PKA R-subunit-encoding gene (pkaR) results in numerous
phenotypic defects similar to those resulting from C subunit deletion, including reduced
hyphal growth, decreased conidiation, and attenuated virulence [5], indicating that proper
regulation of PKA activity is comparably important to A. fumigatus physiology as the
presence of the kinase itself. Given this importance in virulence, we sought to delve into the
specific regulatory mechanisms of A. fumigatus PKA R subunit by examining
phosphoregulation of the protein under optimal growth conditions and cell wall stress
conditions, as well as verify its role in localization of the PKA C subunit. We undertook a
detailed functional analyses of phosphorylation sites identified via liquid chromatographytandem mass spectroscopy (LC-MS/MS) and report marked variance between the
phosphoregulation and function of the PKA R subunit in this filamentous fungal pathogen
compared to mammalian and yeast species. These findings serve to highlight the unique
nature of filamentous fungi and underscore the potential novelty associated with even highly
conserved cellular pathways in this important group of organisms.
MATERIALS AND METHODS
Protein extraction and purification
A. fumigatus recombinant strains expressing wild-type or mutant forms of PkaR-GFP or
PkaC1-GFP fusion protein were cultured in liquid glucose minimal medium (GMM) with
This article is protected by copyright. All rights reserved.
250 rpm shaking for 24 h at 37°C with or without 1g/mL CSP. Total cell lysate was
Accepted Article
obtained by homogenizing mycelia (1g wet weight) using a mortar and pestle as previously
described [23, 24]. Total protein in the crude extracts was quantified by the Bradford method
and samples were normalized to contain 10 mg protein each. GFP-Trap® (Chromotek)
affinity purifications were performed from crude extracts according to the manufacturer’s
instructions as previously described [24].
Phosphopeptide enrichment and LC-MS/MS analysis
Phosphopeptide enrichment and LC-MS/MS were carried out as described previously [13].
Briefly, GFP-Trap® purified samples were processed for TiO2 phosphopeptide enrichment
and mass spectrometry as previously described [23, 24]. Proteolytic digestion was performed
by the addition of sequencing grade trypsin (Promega) directly to the resin. Peptides were
subjected to phosphopeptide enrichment then resuspended and subjected to chromatographic
separation on a Waters NanoAquity UPLC. Samples were analyzed on a QExactive Plus
mass spectrometer using a data-dependent mode of acquisition. MS/MS spectra of the 10
most abundant precursor ions were acquired with a CID energy setting of 27 and a dynamic
exclusion of 20 s was employed for previously fragmented precursor ions.
Qualitative identifications and quantitative analysis of selected ion chromatograms
from raw LC-MS/MS data
Raw LC-MS/MS data files were processed in Mascot distiller (Matrix Science) and then
submitted to independent Mascot database searches (Matrix Science) against a custom
NCBI_Aspergillus database containing both forward and reverse entries of each protein as
described previously [13]. Phosphorylation site localization was assessed by exporting peak
lists
directly
from
Scaffold
into
the
This article is protected by copyright. All rights reserved.
online
AScore
algorithm
(http://ascore.med.harvard.edu/ascore.html). Peak area calculations from extracted ion
Accepted Article
chromatograms were generated within Skyline v3.5 (MacCoss Lab, University of
Washington) following manual peak integration based on identification of retention time and
accurate mass.
Modeling of PkaC1 three dimensional structure
The sequences of the A. fumigatus PKA catalytic subunit and PKA regulatory subunit were
threaded using Coot [25] onto source models with highest sequence homologies, S.
cerevisiae PKA [26] and Mus musculus PKR [27], respectively. Structural figures were
rendered in PyMOL (The PyMOL Molecular Graphics System, Version 1.8 Schrödinger,
LLC.).
Construction of pkaR mutations in Aspergillus fumigatus
Mutant strains were generated as described previously [13]. Briefly, deletion of the gene
encoding PkaR was accomplished by replacement of the pkaR coding region with a selectable
pyrG marker in an akuBKU80 pyrG- auxotrophic strain (See Table S1 for all strains used) via
homologous recombination. Deletion was confirmed via PCR and Southern blot. C-terminal
GFP-labeling of PkaR and PkaC1 was accomplished by insertion of the appropriate coding
region (lacking a stop codon) 5’ to, and in frame with, the egfp coding region within the
pUCGH vector, followed by transformation into the akuBKU80 strain and screening via
hygromycin B selection as described [23]. Site-directed mutagenesis of pkaR was
accomplished by amplifying coding regions of the gene containing the desired point
mutations via fusion PCR as described previously [13]. All mutant strains, as well as the
pkaR-egfp strain expressing GFP-tagged native PkaR protein, were verified for homologous
integration of mutations and egfp coding sequence at the native pkaR locus. Genomic DNAs
This article is protected by copyright. All rights reserved.
amplified from mutant strains using a forward primer targeting a genomic DNA sequence
Accepted Article
upstream of the transformation construct and a reverse primer targeting the egfp coding
region were sequenced to confirm mutations and in-frame gfp fusion. Expression of GFPlabeled PkaC1 in the pkaR deletion background was accomplished by co-transformation of an
akuBKU80 pyrG- auxotrophic strain with linearized plasmids for each modification, followed
my hygromycin B selection of transformants.
Determination of PKA enzyme activity
PKA activity assays were carried out as described previously [13]. Briefly, crude protein
extract normalized by the Bradford method was added in duplicate for each strain to reaction
mixtures containing fluorescent dye-coupled PepTag® (Promega) as a test substrate. Activity
was assessed based on migration of phosphorylated peptide on an agarose gel. For
quantification, gel fragments containing phosphorylated peptide were excised, melted and
fluorescence readings were taken using a 540 nm excitation wavelength in a 96-well plate
using a SpectraMax M3 plate reader (Molecular Devices) and compared to a standard curve.
For statistical analysis, the activity of each mutant strain was compared to that of the pkaRegfp strain via Student’s t-test (P < 0.05).
Assessment of radial growth and CSP sensitivity
For radial growth assays, conidia (104) were point inoculated on solid agar media in petri
plates and incubated for 5 days at 37°C, 40°C or 50°C. Media used included standard GMM
(1% glucose, pH 6.5) as well as modified GMM containing 0 or 0.1% glucose, substitution of
1% sodium acetate for glucose, omission of nitrogen source sodium nitrate, substitution with
alternate nitrogen source ammonium tartrate, and altered pH levels of 4.0 and 8.0. The mean
radial growth rates for each of the strains were compared statistically by Student’s t-test (P <
This article is protected by copyright. All rights reserved.
0.05). For CSP sensitivity determination, CLSI M38-A2 in vitro antifungal susceptibility
Accepted Article
standards were used [28].
Fluorescence Microscopy
Conidia (104) of GFP-labeled strains were cultured on coverslips immersed in 10 mL of
liquid growth medium and incubated for 20 h at 37°C. Alternative growth media were as
described above for radial growth assessment, substituting liquid for solid agar media.
Hyphae were visualized using an Axioskop 2 plus microscope (Zeiss) equipped with
AxioVision 4.6 imaging software
RESULTS
A. fumigatus PkaR is phosphorylated at multiple sites in both caspofungin-dependent
and independent manners
In order to assess the phosphorylation state of PkaR during growth under both optimal and
cell wall stress conditions, two A. fumigatus strains expressing either C-terminally GFPlabeled PkaR (PkaR-GFP) or PkaC1 (PkaC1-GFP) fusion proteins were constructed and each
cultured in the presence or absence of the anti-cell wall antifungal agent caspofungin (CSP;
1µg/mL). The GFP-labeled proteins from each strain were purified by GFP-Trap® affinity
purification (Chromotek) and subjected to LC-MS/MS analysis. In the case of the PkaC1GFP fusion strain, PkaR protein was indirectly isolated based on association with the labeled
catalytic subunit. Thus, phosphorylation sites were identified independently for PkaR protein
isolated through two distinct methods, one direct and one indirect, enhancing the robustness
of the site determinations made. The majority of phosphorylated residues were identified
from PkaR purified using both of these methods. The analysis led to the discovery of 20
potentially phosphorylated serine and threonine residues within the PkaR protein in total.
This article is protected by copyright. All rights reserved.
These were clustered in three primary regions (Fig. 1A), including the less conserved amino-
Accepted Article
terminal portion associated with subcellular localization/targeting in S. cerevisiae [10], the
“hinge” or autoinhibitory region homologous to the catalytic subunit binding domain of other
species [7, 29], and the non-conserved extreme carboxyl terminus. Figure 1B shows amino
acid sequence alignments of phosphorylated regions of A. fumigatus PkaR with other fungal
and mammalian species. Of the sites identified here, S4, T121, S122, S124, S127 and T139
display identity to sites previously reported as phosphorylated in S. cerevisiae [16, 18].
However, with the exception of the S122 homologue, localization of these phosphorylation
sites in the yeast species is imprecise due to the analytical methods employed. Some such
studies have used gel shifts to indicate the presence or absence of phosphorylation without
regard to localization [17, 21] or in conjunction with mutational analysis to identify general
phosphorylated regions [18]. When more sophisticated mass spectroscopic techniques have
been applied, phosphorylation data were reported without analysis of localization
probabilities for specific sites [16]. Nonetheless, general phosphorylation of the N terminal
and hinge regions appears conserved between the two fungal species. Only phosphorylation
of the S122 homologue has previously been reported in mammalian species [21, 22].
Due to clustering of serine and threonine residues in these regions, precise localization of
phosphorylation sites is sometimes difficult. Table 1 shows localization probability scores
(Ascore) for each potential site identified. Of the 20 potential sites, only 10 had localization
scores of 50 percent or higher. Sites with lower scores are likely in some cases to represent
misattributions of adjacent phosphorylation sites with higher scores. For example, the
phosphorylation identified at S30 with a 30.08 localization score may in fact represent
misattributed phosphorylation at S24, which has a localization score of 100.00 and is also
present in the peptide used to identify S30. Sites with lower localization scores frequently
result from random attribution by the analysis software in cases where distinctions cannot be
This article is protected by copyright. All rights reserved.
made between adjacent phosphorylatable residues. Sites with higher localization scores (here
Accepted Article
a score of greater than 50 is used as a cutoff as this indicates nonrandom assignation of a
particular phosphorylation site) presumably represent true distinctions between sites,
indicating that peptides analyzed have been fragmented adequately to isolate individual
phosphorylatable residues, as illustrated in the associated spectrum plots (Fig. S1). Thus, we
considered the 10 identified sites with localization scores above the cutoff value of 50 to be
likely true phosphorylation sites, while those below the cutoff value were considered
indeterminate. In the case of the most N terminal identified phosphorylation, no distinction
could be made between sites S4, S5, S6 or T10, but it is probable that at least one of these
sites was phosphorylated in the analyzed sample.
For relative quantitative comparisons in a non-targeted LC-MS/MS based experiment, only
area-under-the-curve measurements from the exact same modified peptide species (including
position of phosphorylated modification within the full peptide sequence) can be directly
compared across samples. This is due to the fact that peptides of different primary amino acid
sequences or modification positions have different physical chemical properties and therefore
ionize at different efficiencies even if they are present in equal molar quantities. Thus,
statistical comparisons of phosphorylation abundance can only be made between the same
phosphorylated residue across different samples in this study. However, comparison of the
total numbers of peptides identified for multiple sites (that is, the number of times any
peptide was identified displaying phosphorylation at each of the particular residues in
question) can still help to generate hypotheses regarding relative abundance of
phosphorylation at these sites. For this reason we have included these values in Table 1. This
comparison suggests a relatively high degree of phosphorylation at sites S24, S39 and S122,
an intermediate degree of phosphorylation at S407 and S411, and a lower degree of
phosphorylation at the other sites identified. The number of peptides identified for sites
This article is protected by copyright. All rights reserved.
closely adjacent to hypothesized highly abundant sites (e.g. T38 and S39) may be inflated due
Accepted Article
to some of these peptides representing misattribution of phosphorylation at the more
abundant site.
Direct quantitative comparison of phosphorylation levels for the same site across multiple
samples is possible using LC-MS/MS, so we compared abundance of phosphorylated
peptides for selected sites in the presence and absence of 1 g/mL CSP in order to identify
phosphorylation events that might play a role in cell wall stress. Table 2 summarizes the
results of phosphorylation level comparisons between CSP-treated and untreated samples for
PkaR isolated from the PkaR-GFP and PkaC1-GFP-expressing strains. Two replicates were
included for each strain type and each CSP treatment condition. Statistically significant (P <
0.05) dependence of phosphorylation on CSP exposure was identified for several sites.
Phosphorylation was found to be downregulated during CSP exposure for T38 and S39, while
it was upregulated during CSP exposure for S122, S124, S127, S137, T121/S139 dual
phosphorylation, and S407/S411 dual phosphorylation. Upregulation was dramatic in the case
of hinge region phosphopeptides (residues 121-139), ranging from 18.9 to 182.4 fold
increases.
Structural modeling of A. fumigatus PkaR predicts key roles for phosphorylation in
regulation of PkaC1 binding
To determine a structural role for phosphorylated residues in the PKA holoenzyme, the A.
fumigatus PkaC1 and PkaR sequences were threaded onto S. cerevisiae [26] and Mus
musculus source models [27], respectively, as previously reported [13] (Fig. 2).
Phosphorylation of S122 was predicted to have the largest single effect on C subunit binding
due to its proximity to ATP in the holoenzyme structure. According to the model, the side
This article is protected by copyright. All rights reserved.
chain of S122 may be in favorable geometry to accommodate the gamma-phosphate of bound
Accepted Article
ATP and possibly participate in hydrogen bonding with it. Modeling a phosphoserine side
chain at position 122 proved to be difficult due to steric clashes with protein in virtually every
rotameric configuration; thus the phosphoserine was modeled in the singular way that it did
not clash with protein. In this configuration, it clashed head-on with the gamma-phosphate of
bound ATP (Fig. 2, inset). This is expected to cause strong charge repulsion between the
phosphate functional groups of phosphoserine and ATP.
Other phosphorylated residues including T121 and S124 may influence holoenzyme
formation and function with less impact. Specifically, T121 has its side chain oriented toward
solvent in the structure, so while a phosphothreonine at position 121 would avoid steric clash,
there may still be some charge repulsion with nearby ATP. S124 represented the opposite
case: with a phosphoserine at position 124, significant charge repulsion would not be
expected due to the distance from the ATP triphosphate moiety, however the additional
electronic bulk of a phosphoserine side chain may alter the PkaC1-PkaR interface in ways
that could affect function. It is notable that phosphorylation at this cluster of residues is most
closely associated with the PkaC1-PkaR interface (also including S127) and is also strongly
increased in response to CSP exposure (Table 2).
S24 and S39 were unfortunately not in any available structural models. They are part of the
conserved regulatory subunit N-terminal dimerization domain (“D/D”) that is connected to
the inhibitory polypeptide region via flexible linker ([30, 31]. Since evidence suggests the
D/D interaction to be structurally symmetrical, it may be that phosphorylation of S24 and S39
could cause steric clash and/or charge repulsion with their equivalents thus disrupting the
assembly of subunits in some way. The serine and threonine residues spanning positions 127-
This article is protected by copyright. All rights reserved.
139 were not involved in either the PkaC1-PkaR holoenzyme or the quaternary tetrameric
Accepted Article
protein structure, reflecting a diminished potential role for their rates of phosphorylation.
S407 and S411 were found to be phosphorylated with greater than 50% localization
probability though they are not in the PkaC1-PkaR interface region. These residues are part
of the C-terminal domain that is conserved and associated with cAMP binding. The extreme
C-terminal portion bearing these residues is highly variable and the structural significance of
phosphorylation in that region remains unclear.
Phosphorylation of PkaR contributes to hyphal growth and cell wall stress tolerance
In order to assess the functional role of phosphorylation at identified sites, site-directed
mutagenesis was used to generate strains expressing mutant PkaR isoforms with nonphosphorylatable alanines substituted for selected phosphorylated residues. In the N-terminal
domain, sites S24 and S39 were selected for examination due to the high number of
phosphopeptides identified for these sites (Table 1), which would seem to suggest abundant
phosphorylation and therefore a greater likelihood of functional significance. A strain was
therefore generated expressing PkaR with alanine substitutions at these residues
(PkaRS24A;S39A). In the hinge region, S122 was targeted for mutagenesis due to both the
abundance of phosphopeptides identified and because the site is homologous to the conserved
autoinhibitory sites of yeast and mammalian PKA regulatory subunits, in which systems its
phosphorylation is of known functional importance. For this site, strains were generated
expressing substitutions to both alanine (PkaRS122A) and glutamate (PkaRS122E), the latter
meant to represent a constitutively phosphorylated state, as constitutive phosphorylation and
dephosphorylation at this site might be expected to have unique functional consequences due
to altered catalytic subunit interaction. Additionally, because both S122 and the adjacent
predicted phosphorylation sites T121 and S124 were found to exhibit increased
This article is protected by copyright. All rights reserved.
phosphorylation during CSP exposure (Table 2), a mutant strain was generated in which all
Accepted Article
three sites were mutated to alanines (PkaRT121A;S122A;S124A) to examine the possibility that
tandem phosphorylation of this amino acid cluster is required to produce significant
physiological effects. Finally, the S158 residue located at the junction between the hinge
region and cAMP binding domains, was targeted for mutagenesis to alanine (PkaRS158A) due
to the high localization probability score associated with this site and because
phosphorylation has not previously been identified in homologous areas of other species
studied (Fig. 1B), suggesting the potential for novel functionality. Sites within the extreme Cterminal region were not pursued for further examination in this study due to the fact that
phosphorylation at these sites was not identified through analysis of directly purified GFPlabeled PkaR, but only through analysis of native PkaR indirectly purified through
association with GFP-labeled PkaC1, possibly due to inhibition of phosphorylation at these
sites by the presence of the C-terminal GFP label. The lack of any discernable phenotypic
variation between strains expressing native PkaR versus PkaR-GFP (Fig. S2) despite the
apparent absence of C terminal phosphorylation in the latter strain argues against functional
significance of phosphorylation in this region. All PkaR mutant isoforms were C terminally
labeled with GFP to aid in downstream analyses and comparisons were made to a strain
expressing GFP-labeled native PkaR.
Strains expressing the described PkaR mutant isoforms were examined for altered PKA
activity based on phosphorylation of a fluorescently labeled PepTag substrate peptide
(Promega) added to whole cell extracts from each mutant and compared in duplicate to PkaRGFP (WT; positive control) and PkaR deletion (pkaR) strains, as well as a PkaC1 deletion
strain (pkaC1) [13] as a negative control. While none of the mutant strains exhibited
significantly altered catalytic activity, phosphorylation of the target peptide was reduced by
This article is protected by copyright. All rights reserved.
approximately half in the pkaR strain compared to WT in a statistically significant manner
Accepted Article
(Fig. 3A, B).
PKA activity has been previously associated with alternate carbon source utilization and heat
stress tolerance in A. fumigatus [6, 13], carbon and nitrogen stress responses in S. cerevisiae
[32, 33] and pH stress response in Cryptococcus neoformans [34]. To determine whether
PkaR phosphorylation plays a role in hyphal growth under normal or stress-inducing
conditions, the PkaR phosphorylation mutant strains were further examined for altered radial
growth under optimal growth conditions on glucose minimal medium (GMM) as well as
growth on the non-fermentable carbon source acetate, under carbon and nitrogen starvation
conditions, heat stress, and alkaline and acidic pH stresses. Of the strains examined, the
PkaRS24A;S39A, PkaRS24A;T38A;S39A and PkaRT121A;S122A;S124A mutants demonstrated slight but
significant (P < 0.01) growth defects under optimal growth conditions (Fig. 4A, B) that were
not exacerbated disproportionately to the control strain under any of the stress conditions
tested (Fig. S3). None of the other strains exhibited significant growth defects compared to
the control strain under any conditions tested, including the single alanine and glutamate
substitution mutants at the S122 locus.
Mutants were also tested for cell wall stress tolerance by exposure to various concentrations
of CSP (Fig. 5). The pkaR strain showed markedly attenuated growth during exposure to
CSP concentrations as low as 0.25 µg/mL compared to the control strain, and did not display
paradoxical growth recovery [35] at higher concentrations, as observed in the wild-type strain
at 4 µg/mL. Of the mutant strains, only the PkaRT121A;S122A;S124A strain displayed increased
sensitivity to caspofungin. The growth pattern of this mutant was noticeably more aberrant
than the wild- type strain at CSP 0.25 ug/mL, and hyphal elongation was markedly attenuated
compared to the wild-type strain at CSP 0.5 ug/mL. While some paradoxical growth recovery
This article is protected by copyright. All rights reserved.
was observed in this strain at higher CSP concentrations, it was less pronounced than in the
Accepted Article
wild-type strain. Although phosphorylation levels of S122 had been found to increase
markedly during CSP exposure via LC-MS/MS, neither the PkaRS122A or PkaRS122E mutant
strains demonstrated altered sensitivity to the compound. Thus, mutation of this site alone
was insufficient to alter CSP sensitivity.
Loss of PkaR does not influence localization of PkaC1 under basal or stress-inducing
growth conditions
As a primary function of the PKA regulatory subunit in both mammalian and yeast species
studied appears to be directing the subcellular localization of the catalytic subunit, we
examined the localization patterns of GFP-labeled PkaC1 in both native (WT) and PkaRdeletion (pkaR) genetic backgrounds via fluorescence microscopy grown for 24 hours under
a variety of conditions including: basal (1% glucose), glucose limitation (0.1% glucose),
glucose starvation (0% glucose), alternative nonfermentable carbon source (sodium acetate),
alternative nitrogen source (ammonium tartrate), nitrogen starvation, pH4, and pH8. In
contrast to observations reported in yeast species, we found primarily cytoplasmic
localization of PkaC1 under all the conditions examined, and loss of PkaR did not have any
clear impact of PkaC1 localization. Figure 6 shows representative images of PkaC1
localization in both genetic backgrounds during growth in the presence of various glucose
concentrations (data not shown for alternative growth conditions).
This article is protected by copyright. All rights reserved.
DISCUSSION
Accepted Article
We have defined novel mechanisms through which the PKA regulatory (R) subunit of A.
fumigatus is regulated. We identified numerous novel sites of phosphorylation within this
important protein, including two phosphorylation clusters with roles in regulating hyphal
growth and/or the cell-wall integrity of the fungus. This is the first report identifying specific
phosphorylation sites for the PKA R subunit of a filamentous fungus. For the majority of sites
identified in this study, homologous sites in other species are not known to be phosphorylated,
indicating the potential for both novel mechanisms of phosphorylation and unique
downstream effects on PKA function. In further support of this concept, we have
demonstrated that localization of the catalytic (C) subunit in this species is independent of the
regulatory subunit across a range of growth conditions, in contrast to the established roles of
PKA R subunits in both mammalian and yeast species.
Similarly to findings in S. cerevisiae, we identified clustered phosphorylation in the Nterminal portion of PkaR, within the less-conserved region upstream of the autoinhibitory
hinge region where C subunit binding occurs. In S. cerevisiae, phosphorylation of this region
has been shown to be important for the nuclear localization of the R subunit Bcy1 [18].
However, the amino acid sequence of this region in A. fumigatus PkaR bears only a low
degree of similarity to the corresponding region of Bcy1, and the specific phosphorylation
sites identified are not generally conserved between the two species (Fig. 1). Thus, it is
plausible that phosphorylation of this domain may play a different role in A. fumigatus. We
found that mutation of the two sites in this region most heavily phosphorylated based on
peptide counts (S24 and S39, Table 1) resulted in slight but significant attenuation of radial
growth. This appeared to be a general defect in hyphal growth and was not exacerbated
disproportionately during growth under any of the environmental stresses examined. While
additional mutation of T38 did not influence the phenotype further, it is possible that
This article is protected by copyright. All rights reserved.
phosphorylation at the various identified sites within this region may function redundantly, so
Accepted Article
that mutation of a greater number of sites would be required to produce a strong phenotypic
effect. In the future, it would be of interest to examine the physiological impact of truncating
this region in order to better assess its importance and specific role in PKA function, as has
been previously performed in budding yeast [10].
The identification of phosphorylation within this N terminal domain homologous to the
targeting region of yeast raised the question of whether this phosphorylation would play a
similar role in A. fumigatus. However, we were unable to identify a role for PkaR in
controlling PkaC1 subcellular localization under conditions in which such a role has been
previously established in S. cerevisiae, C. albicans and the fission yeast S. pombe. In these
yeast species, shuttling between the nucleus and cytoplasm in response to environmental
factors, particularly glucose abundance, appears to be an essential component of PKA
function. In this study, we found that under all conditions examined PkaC1 remained
primarily cytoplasmic, and that deletion of PkaR did not have a discernable effect on
localization. This result is thus indicative of major discrepancy between the function of PKA
R subunits in the yeasts and A. fumigatus. As the R-subunit-dependence of PKA localization
has not previously been examined in a filamentous fungus, it is possible that this may
represent a divergence between yeast species and filamentous fungi in general.
While phosphorylation of the highly conserved autophosphorylation site residue S122 was
identified in our study, mutation of this site alone to either a non-phosphorylatable alanine or
a phosphomimetic glutamate had no discernable phenotypic effects. This sharply contrasts
with findings in yeast, in which the homologous site was identified as integral to regulating
the stability of R and C subunit interaction and the mutation of which had pronounced
physiological impact [20, 36]. In A. fumigatus, we found that mutation of additional
This article is protected by copyright. All rights reserved.
phosphorylation sites within this hinge region was required in order to observe phenotypic
Accepted Article
alteration. These observations would also seem to be inconsistent with predictions based on
our structural modeling (Fig. 2), wherein S122 phosphorylation alone was expected to
strongly inhibit interaction between PkaR and ATP-bound PkaC1, which would presumably
result in significant dysregulation of PKA activity and commensurate phenotypic effects in
the PkaRS122E mutant strain. The phenotypic effect produced through the mutation of adjacent
phosphorylation sites in addition to S122 reflects possible structural differences between A.
fumigatus PkaR and/or PkaC1 in comparison to the source model structures used, resulting in
altered biochemical interactions between the subunits compared to those predicted. As in the
case of the N terminal region of the protein, phosphorylation of this hinge region may exhibit
functional redundancy between specific sites. As in yeast, this phosphorylation may act to
destabilize R and C subunit interaction. The strong increase in phosphorylation observed in
this region during caspofungin exposure is of particular interest. As we found a notable
increase in sensitivity to this antifungal compound in the PkaRT121A;S122A;S124A triple mutant, it
would be worthwhile to determine whether mutation of the other caspofungin-dependent
phosphorylation sites in this region would result in even greater sensitivity. It is possible that
phosphorylation of the PkaR hinge region during caspofungin exposure may serve to enhance
PKA activity to a minor degree through reduced R subunit binding, promoting tolerance to
cell wall stress through undetermined mechanisms. Better understanding of the mechanisms
involved in regulating this response could potentially lead to new methods for enhancing the
efficacy of caspofungin and other cell-wall-targeting agents.
In order to explore possible pathways involved in PkaR phosphoregulation, we performed a
bioinformatic analysis using the NetPhos 3.1 server [37] to identify potential kinases
involved in phosphorylation at sites found to be involved in hyphal growth and/or
caspofungin tolerance (Table 3). For the S24, T38, S39 cluster, moderate hits were produced
This article is protected by copyright. All rights reserved.
for both MAP (p38 MAPK) and casein (CKI) kinases. The prediction of MAP kinase
Accepted Article
involvement is bolstered by the reported direct phosphorylation of the targeting region of S.
cerevisae Bcy1 by MPK1[16], homologous to A. fumigatus MpkA, a key regulator of the cell
wall integrity (CWI) pathway [38]. This result thus suggests potential cross talk between the
cAMP and CWI pathways, or possibly involvement of the high osmolarity glycerol response
pathway, regulated by the MAP kinase SakA [39, 40]. Analysis of the hinge region cluster
comprising T121, S122 and S124 produced moderate hits for both calcium/calmodulindependent protein kinase (CaM Kinase-II) and glycogen synthase kinase (GKS3) at all three
sites (though in the case of S122 the strongest prediction was for PKA, as expected).
Interestingly, in addition to CaM kinases, both the GSK3 homologue GskA and the CKI
homologue CkiA have been found to play important roles in the calcium signaling pathway
of Aspergillus nidulans [41]. Thus, calcium signaling might potentially be involved in
regulating phosphorylation of both the hinge and targeting region clusters of PkaR. More
research is needed to verify these possibilities.
This work represents the first foray into understanding the regulation of PKA R subunit
function in filamentous fungi. Our findings highlight important differences between the
mechanisms of PKA regulation in A. fumigatus compared to mammals and previously
examined yeast species. Though R subunit phosphorylation as a means of PKA regulation is
common to each group, the specific regulatory functions appear to be quite different. While
this work provides new insight into PKA regulation in filamentous fungi, questions
concerning the precise mechanisms through which R subunit phosphorylation impacts PKA
function remain to be resolved in future studies. Though the basic PKA signaling mechanism
is conserved throughout the eukarya, control of this activity through diverse R subunit
isoforms represents a highly versatile component to this essential pathway and a promising
focus for identifying key variations between signaling among different taxa. Elucidating the
This article is protected by copyright. All rights reserved.
functional importance of the PKA R subunit and how its functions are specifically regulated
Accepted Article
through phosphorylation and other mechanisms in A. fumigatus will therefore represent an
important step forward in our overall understanding and eventual targeting of this important
human fungal pathogen.
FUNDING INFORMATION
These studies were supported by the Tri-Institutional Molecular Mycology and Pathogenesis
Training Program (2T32 AI052080-11) post-doctoral fellowship to EKS and NIH/NIAID
multi-PI R01 award AI112595 to WJS.
FIGURE LEGENDS
Figure 1. Multiple sites of phosphorylation identified in PkaR by mass spectrometry.
(A) Diagram showing locations of phosphorylated sites within the PkaR protein. Residues
highlighted in green were identified by LC-MS/MS as phosphorylated with greater than 50%
localization probability, while residues highlighted in gray had less than 50% localization
probability. (B) Clustal W alignment of phosphorylated regions of PKA regulatory subunits
of selected fungal and mammalian species. Amino acids highlighted in blue indicate their
conservation in relation to A. fumigatus PkaC1. Amino acids highlighted in green indicate
residues that have been experimentally determined to undergo phosphorylation in the
associated organism. Numbering of the residues showing the respective phosphorylation is
based on the A. fumigatus protein.
This article is protected by copyright. All rights reserved.
Figure 2. Serine and threonine residue phosphorylation in A. fumigatus PkaR. The
Accepted Article
threaded Aspergillus fumigatus PkaR-PkaC1 model is shown with the PkaC1 polypeptide
backbone in white, the PkaR backbone in violet, and the ATP cofactor in atom colors. Serine
and threonine residues found to be phosphorylated with greater than 50% localization
probability are highlighted in green while those with less than 50% are highlighted in cyan.
The inset shows the effect of a phosphorylated Ser122 residue: massive steric clash and
charge repulsion with the gamma-phosphate of bound ATP.
Figure 3. Impact of PkaR phospho-mutations on protein kinase activity. (A) Protein
normalized crude cellular extracts for each mutant strain were added in duplicate to reaction
mixtures containing fluorescent dye-coupled PepTag peptide (Promega) as a PKA-specific
test substrate and incubated for 30 minutes at room temperature. Activity was assessed
qualitatively based on migration of negatively charged, phosphorylated peptide towards the
anode of an agarose gel. Results shown for representative set of strains. (B) Activity was
quantified by comparing fluorescence of phosphorylated substrate for each sample to a
standard curve using a plate reader and the average values for each mutant were plotted.
Activity was defined as pmol of substrate phosphorylated per minute. Error bars represent
standard deviation. Activities were compared to the PkaR-GFP (WT) control via Student’s ttest and statistically significant differences (P < 0.05) are indicated by asterisks above
columns. Other than the pkaC1 negative control, only the pkaR strain demonstrated
significantly altered PKA activity.
Figure 4. Radial growth of A. fumigatus pkaR mutants. (A) Conidia of the indicated
strains were point inoculated onto GMM agar plates and incubated for five days at 37°C. WT
indicates control strain expressing GFP-tagged native PkaR (PkaR-GFP). (B) Quantitation of
This article is protected by copyright. All rights reserved.
radial growth. Columns represent an average of three to eight replicates and error bars
Accepted Article
represent standard deviation. Asterisks above columns represent statistically significant
differences compared to WT as determined by Student’s t-tests (P < 0.05). Significant growth
defects
were
identified
for
the
DpkaR,
PkaRS24A;S39A,
PkaRS24A;T38A;S39A,
and
PkaRT121A:S122A:S124A strains.
Figure 5. Caspofungin tolerance of A. fumigatus pkaR mutants. Sensitivity of WT and
PkaR mutants to caspofungin (CSP). 102 conidia were inoculated into liquid RPMI medium
containing 0, 0.25, 0.5, 1, or 4 µg/mL of CSP and incubated at 37°C for 48 hours. The pkaR
strain and a strain deficient for phosphorylation at three phosphorylated hinge region residues
showed increased sensitivity to CSP compared to WT. PkaR deletion also resulted in a loss of
paradoxical growth recovery at higher CSP concentrations. Scale bars represent 100 mm.
Figure 6. Localization PkaC1 in WT and DpkaR strains. Conidia (104) of strains
expressing GFP-labeled PkaC1 (PkaC1-GFP) in wild-type (WT) and pkaR gene deletion
(pkaR) backgrounds were cultured on coverslips immersed in liquid broth with the indicated
glucose concentrations and incubated for 24 h at 37°C. Hyphae were visualized using an
Axioskop 2 plus microscope (Zeiss) equipped with AxioVision 4.6 imaging software.
Differential interference contrast (DIC) images are shown to the left, while GFP fluorescence
(PkaC1-GFP) images are shown to the right. Scale bars indicate 10 mm distances. In all
instances, PkaC1 localization appeared to be primarily cytosolic.
This article is protected by copyright. All rights reserved.
Figure S1. Spectra indicating phosphorylation at specific PkaR residues. Tandem mass
Accepted Article
spectra of peptides from PkaR reveal ten unique phosphorylated serine residues. The
presence of each identified C-terminal (y) and N-terminal (b) product ions are indicated
within the peptide sequence. Peaks indicating phosphorylation are represented in yellow.
Figure S2. Radial growth of A. fumigatus pkaR-GFP and PkaR mutant strains. (A)
Comparison of hyphal growth in strains expressing native or GFP-tagged PkaR. Conidia of
the pkaR-egfp strain (expressing C-terminally GFP-tagged PkaR, PkaR-GFP) and its parent
strain akuBKU80 were point inoculated onto GMM agar plates and incubated for five days at
37°C in triplicate. No significant differences in radial growth rate were measured between
the two strains. Representative plates are shown. (B) Quantitation of radial growth of
selected PkaR mutants under stress conditions. Conidia of the indicated strains were point
inoculated onto agar plates and incubated for five days. “Standard” refers to growth at 37 C
on GMM (pH 6.5). The “40 C” and “50 C” groups were grown on GMM at those
temperatures. For the “pH 4” and “pH 8” groups, GMM was adjusted to the indicated pHs.
For the “Acetate” group, sodium acetate (1%) was substituted for glucose in the medium.
Columns represent an average of 8 replicates for standard conditions and 3 replicates for all
other conditions. Error bars represent standard deviation. Asterisks above columns
represent statistically significant differences in comparison to the WT (PkaR-GFP) control as
determined by Student’s t-tests (P < 0.05). For the stress conditions depicted here and
others not shown but referenced in the text, only the DpkaR strain showed significantly
altered growth in comparison to the WT control.
This article is protected by copyright. All rights reserved.
ccepted Articl
Phosphorylated
Residue
S4, S5,
S6, or
T10
S17
Peptide Sequencea
m/z
Charge
Mass
Error
(ppm)
Mascot
Ion
Scoreb
Ascore
Localization
Probabilityc
Total
Peptides
Identified
MADSS[pS]FPGTNPFLK
774.3443
0
2.30
10.3
N/A
1
V[pS]TKDDKYSPIQK
530.2598
794.8847
2
2
1.97
0.20
22.9
68.2
67.89
100.00
2
101
1002.4272
1104.9654
1104.9645
977.0815
950.4237
713.0707
1
0
0
1
1
1
-2.28
0.73
-0.15
2.58
0.20
1.71
19
39.5
53.9
16.1
67.5
49.4
30.08
56.11
87.25
39.79
78.54
73.44
4
8
468
1
61
8
923.9208
2
-0.84
21.5
37.04
8
898.3883
898.3907
977.0815
735.9932
676.7689
558.7351
636.7849
0
0
1
0
1
0
1
-1.68
0.97
2.58
2.19
1.68
1.76
0.36
25.7
16.5
16.1
45.6
29.8
39.9
24.3
17.48
15.14
38.06
95.38
99.31
94.14
59.75
2
5
1
4
12
27
5
S24
VSTKDDKY[pS]PIQK
S30
T38
S39
S121
S122
S124
KYSPIQKI[pS]EEEEYEVT[pS]PTDPTFR
ISEEEEYEV[pT]SPTDPTFR
ISEEEEYEVT[pS]PTDPTFR
R[pT]SVSAESLNPTSAGSDSW[pT]PPCHPK
RT[pS]VSAESLNPTSAGSDSWTPPCHPK
RTSV[pS]AESLNPTSAGSDSWTPPCHPK
RTSVSAE[pS]LNPTSAGSDSWTPPCHPKTEEQLSR
RTSVSAESLNP[pT]SAGSDSWTPPCHPK
TSVSAESLNPTSAGSD[pS]WTPPCHPK
R[pT]SVSAESLNPTSAGSDSW[pT]PPCHPK
TAV[pS]NNFLFSHLDDDQFR
RAEY[pS]AKP[pS]PS
AEYSAKP[pS]PS
RAEYSAKPSP[pS]
S127
S131
S137
S139
S158
S407
S411
S413
Table 1. PkaR Phosphorylation Sites Identified by LC-MS/MS Analysis.
a
[pS/T] indicates phosphorylated residue
Mascot identity score of > 41 indicates identity or extensive homology (p < 0.05)
c
Probability of phosphorylated residue localization based on Ascore algorithm
b
This article is protected by copyright. All rights reserved.
ccepted Articl
Table 2. Caspofungin-Dependence of Phosphorylation at Selected PkaR Sites Identified by LC-MS/MS Analysis.
Phosphorylated
Residue
Peptide Sequencea
PkaC1-GFP
CSPPeak Area
PkaC1-GFP
CSP+
Peak Area
PkaC1-GFP
Fold
Change
PkaR-GFP
CSPPeak Area
PkaR-GFP
CSP+
Peak Area
PkaR-GFP
Fold
Change
S17
V[pS]TKDDKYSPIQK
S24
VSTKDDKY[pS]PIQK
1.43e9
1.43e9
1.00e9
9.95e8
-1.4
-1.4
3.43e9
3.45e9
1.78e9
1.80e9
-1.9
-1.9
3.04e10
3.04e10
2.16e6
6.39e7
6.39e7
254e10
2.54e10
1.13e7
6.31e9
6.10e9
-1.2
-1.2
52.3
98.7
95.4
5.93e10
5.93e10
1.52e5
5.71e8
5.72e8
2.24e10
2.24e10
2.77e7
1.08e10
1.08e10
-2.7*
-2.6*
182.4*
18.9*
18.9*
3.38e7
2.07e7
6.42e7
6.47e6
4.82e8
3.14e7
6.31e9
1.80e7
14.3
1.5
98.3
2.8
1.46e7
8.17e7
5.71e8
9.16e8
4.09e7
1.08e10
2.44e7
1.54e7
62.9*
-2.0
18.9*
-1.6
4.33e8
1.24e9
6.17e9
6.66e8
1.03e9
5.72e9
1.5*
-1.2
-1.1
2.20e6
2.12e6
8.08e6
2.32e5
5.79e6
5.22e6
-9.5
2.7
-1.5
T38
ISEEEEYEV[pT]SPTDPTFR
ISEEEEYEVT[pS]PTDPTFR
S39
S121;S139 R[pT]SVSAESLNPTSAGSDSW[pT]PPCHPK
RT[pS]VSAESLNPTSAGSDSWTPPCHPK
S122
RTSV[pS]AESLNPTSAGSDSWTPPCHPK
S124
S127
S131
S137
S158
TSVSAE[pS]LNPTSAGSDSWTPPCHPK
RTSVSAESLNP[pT]SAGSDSWTPPCHPK
TSVSAESLNPTSAGSD[pS]WTPPCHPK
TAV[pS]NNFLFSHLDDDQFR
S407;S411
RAEY[pS]AKP[pS]PS
AEYSAKP[pS]PS
S411
RAEYSAKPSP[pS]
S413
* Indicates statistical significance (P<0.05).
This article is protected by copyright. All rights reserved.
Table 3. Potential Kinases Targeting PkaR Phosphorylation Sites
Accepted Article
Phosphorylation Site
S24
T38
S39
T121
S122
S124
Kinases
CKI
p38
MAPK
GSK3
NetPhos Prediction Score
0.525
CKI
p38
MAPK
CKI
0.52
0.532
GSK3
0.495
GSK3
0.444
CaM-II
0.429
PKA
0.794
CaM-II
0.494
GSK3
0.431
CaM-II
0.474
GSK3
0.441
0.489
0.483
0.519
This article is protected by copyright. All rights reserved.
Accepted Article
This article is protected by copyright. All rights reserved.
Accepted Article
This article is protected by copyright. All rights reserved.
Accepted Article
This article is protected by copyright. All rights reserved.
Accepted Article
This article is protected by copyright. All rights reserved.
Accepted Article
This article is protected by copyright. All rights reserved.
Accepted Article
This article is protected by copyright. All rights reserved.
Документ
Категория
Без категории
Просмотров
3
Размер файла
1 484 Кб
Теги
12886, 1873, 3468
1/--страниц
Пожаловаться на содержимое документа