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2004
Nucleic acids
U 0700
Base-Discriminating Fluorescent (BDF) Nucleoside: Distinction of Thymine by
Quenching. — A novel fluorescent probe containing pyrene-labeled
50- 176 Fluorescence
7-deaza-2'-deoxyadenosine (VI) is developed for the detection of thymine base on a
target DNA. Key step is the Sonogashira coupling of substituted propargyl amine (II)
to 7-iodo adenosine derivative (I). After protection of the deoxyribose 5'-hydroxyl
group and conversion to the phosphoramidite, the nucleoside is incorporated into oligonucleotides. — (SAITO, Y.; MIYAUCHI, Y.; OKAMOTO, A.; SAITO*, I.; Chem.
Commun. (Cambridge) 2004, 15, 1704-1705; NEWCAT Inst., Fac. Eng., Nihon Univ.,
Japan Sci. Technol Corp., Tamura, Koriyama 963, Japan; Eng.) — M. Paetzel
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