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BY (11) 11635
(13) C1
(46) 2009.02.28
(19)
(12)
(51)
UREAPLASMA
MYCOPLASMA HOMINIS
(54)
UREALYTICUM
(21)
(22) 2006.05.16
(43) 2007.12.30
(71)
:
: a 20060463
(73)
:
"
-
-
"
" (BY)
(72)
(2006)
C 12Q 1/68
:
" (BY)
(56) WO 97/46963 A1.
JP 2001245697 A, 2001.
JP 11089597 A, 1999.
;
;
;
(BY)
(57)
Ureaplasma urealyticum
urealyticum
BY 11635 C1 2009.02.28
0,2
2�3
urealyticum
,
Mycoplasma hominis,
, 20
2�7
5
2�
,
0,02
2
2�4
Mycoplasma hominis
Ureaplasma urealyticum
Mycoplasma hominis,
Ureaplasma
,
2�6
,
,
0,002
Ureaplasma
1:1.
,
Mycoplasma hominis
-
,
(
.
)
-
BY 11635 C1 2009.02.28
Ureaplasma urealyticum
MyMycoplasma hominis,
-
coplasma hominis
Ureaplasma urealyticum
,
.
Ureaplasma urealytiUreaplasma urealyticum
cum
Mycoplasma hominis
Mycoplasma hominis,
,
Ureaplasma urealyticum
My-
coplasma hominis
(R2)
( ) ? 0,89.
? 0,9,
log 10
.
Ureaplasma urealyticum
hominis,
,
plasma urealyticum
Mycoplasma hominis,
,
, 20
2�7
5
0,2
2�
,
0,02
2�3
urealyticum
Mycoplasma hominis
2
4
2�
,
Mycoplasma
Urea6
2�
,
0,002
Ureaplasma
1:1.
.
-
,
,
1-
,2-
.
("BIOMERIEUX",
Ureaplasma urealyticum
Ureaplasma urealyticum
)
-
.
Genomic DNA Miniprep Kit (Sigma,
,
GenElute? Mammalian
.
,
-
)
.
(
)
Ureaplasma urealyticum
1:
C� N
32,1� 6,02E + 23
C =
,
,C =
= 8,0 �10
6
Gs � Mw
751719,0 � 321,4 �
Ureaplasma urealyticum
2�7
.
103
Ureaplasma urealyticum 2�
.
260
=
20
0,002
260
� k,
.
-
2:
,
= 0,762� = 38,1
.
-
10.
1:1
2� - 2�3
.
5- 10,0, 1,0, 0,1, 0,01
Ureaplasma urealyticum
Ureaplasma urealyticum
5-
7
20,0-0,002
/
5,
Ureaplasma urealyticum
1� , 1� , 1� , 1�4 1�3
,
0,001
.
7
2
6
5
BY 11635 C1 2009.02.28
Ureaplasma urealyticum.
5Ureaplasma urealyticum 1�7, 1�6, 1�5, 1�4
1�3
10,0, 1,0, 0,1, 0,01 0,001
.
Ureaplasma urealyticum
"
-FRT Ureaplasma urealyticum".
-1
7
,
-1,
10
7
3
Ureaplasma urealyticum
1� - 1�
2,
(NTC). NTC
,
.
"Rotor-Gene-3000"
: 95 � 5
; 95 � - 20
, 65 � - 20
; 72 � - 20
, 10
; 95 � - 20
, 60 � - 30
,
72 � - 15
, 35
.
NAGK,
Ureaplasma urealyticum,
: 5'-TGGGCAGACACATCGTAGCA-3'
(NAGK -1), 5'-3' (NAGK -2), 5'-VICTGTTGCCCGAGATTGACCCGGT-TAMRA-3'.
Eppendorf
0,2
15
,
6,85
, 2,5
(100
Tris-HCl (pH 8,4), 50
l), 4
25 mM MgCl2, 0,2
25
dNTP, 1,25
,
20TagMan(
- 300
200
) 0,2
Taq.
25
.
10
,
- 10
.
3-5
15
. ./
.
"Rotor-Gene-3000"
: 95 � - 5
, 95 � - 15
,
60 � - 60
; 50
.
NTC (No Template Control). NTC
,
.
Ureaplasma urealyticum
NAGK
NTC.
(R2)
log 10
Ureaplasma
urealyticum (
)
log 10
NAGK
),
Ureaplasma urealyticum,
0,99 0,98
.
0,91
Ureaplasma urealyticum 0,89
NAGK.
,
:
Ureaplasma urealyticum
Mycoplasma hominis,
,
,
2
(R )
log 10
? 0,9
( ) ? 0,89
.
.
220034, .
,
.
, 20.
3
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