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Патент USA US2112507

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2,112,507
‘Patented Mar. 29, 19-38 _
' UNITED STATES
PATENT OFFICE» §
2,112,507
_
PROCESS OF PRODUCING VACCINE
LYMPH.
.
Klshiro Sukegawa, Koishikawa-ku, Tokyo, Japan
No Drawing. Application June 29; 1935, Serial
No. 29,175. In Japan December 28, 1933
5Claims.
This invention relates to a process of produc
ing vaccine lymph, and consists in adding to meat
Juice a suitable decomposed albumin, e. g; pepton
or amino-acid, and salt and sugar, neutralizing
5 it with colloidsol of sodium silicate or alkali-car
bonate, adding hormone thereto, next adding ac,
tive elements oi.’ bird's ovum obtained during the
process of it's hatching, thereby obtaining a cul
tural media, and then transmitting and culti
10 vating the virus of smallpox in said cultural
medium.
‘
l The object of the invention is to cultivate the
virus of smallpox by feeding them arti?cially,
without relying upon the animal body as in the
15 process heretofore practised, thereby obtaining,
in a simple and economical manner, the virus of
smallpox necessary for the vaccination into the
human body, whose reaction upon the skin is mild
when inoculated upon the human body and whose
20
protective power keeps long potent.
According to this invention, the liquid of meat
juice is ?rst added with a suitable decomposed
albumin (e. g. pepton or amino-acid), salt and
sugar such as grape-sugar or milk-sugar.
The
5 mixture is neutralized by adding thereto colloid
(01. 167-78),
quantity of the active elements of the ovum, i. e.
embryonic ectoblast tissue, and plasma of the
choroid urethral membrane obtained on the 8th
day during the incubative process of a hen’s egg.
Then the legal vaccine lymph is transplanted and is
cultivated in the culture media thus prepared for
10 days in the incubator at 37° C. Then the prod
uct is ?ltered, and thereto is added 0.5% glycer
inum of carbolic acid, thereby producing the pure,
sterilized vaccine lymph.
l0
In'carrying’out my process, I may use amino
acid in place of pepton, milk-sugar in place of »
grape-sugar, egg of duck or quail in place of egg
of hen, hormone of thyroid-gland or hypophysis
in place of adrenalin, sodium silicate, with the 16
same satisfactory result.
I may also use a solid‘
culture media, which is made by adding Japanese
gelatine to the above mentioned liquid nutriment.
A historical record of the methods of produc
ing the vaccine lymph is as follows:(A) _
, 20
VARIOLATION
This method was prevalent in India and China
in ancient times and consists in inoculating the
toxin oi smallpox directly upon the human body. 25
sol of sodium silicate or alkali-carbonate. Then, vBut this method often provokes actual case.
to said neutralized liquid is added some sort of
(B)
VACCINATION
hormone body, e. g. such as pituitary body, or
thyroid gland of hormone, or some chemical com
This is known as “Jenner’s method", for which
the following four kinds of virus of smallpox are 30
30' pound which has the equivalent e?ect, and there
.
to is also added the ef?cacious element of bird's in use:
ovum obtained on the third day or on any day up
(1) Humanized vaccine
to the twelfth day during the process of its hatch
This is’ a matter taken from the vesicle pro
ing (e. g. in the case oi.’ a hen’s egg‘, embryonic
duced by inoculating the germs of smallpox of a
35 ectoblast tissue and plasma of the choroid ure
thral membrane, or an extract thereof), thereby
obtaining a liquid or solid cultural medium.
Then, the virus of smallpox, namely the vaccine
lymph separated from miscellaneous germs (the
40 vaccine lymph established according to the pres
ent regulation, or retrovaccination cow lymph
established similarly) is transplanted and culti
vated in the cultural media prepared in the man
ner mentioned above.
45
An example of the mode of carrying out this
invention is as follows:
To 100 parts of meat juice, there are added
from 10 to 15 parts of the decomposed albumin,
e. g. pepton or amino-acid, from 5 to '7 parts 0!
50 salt, and from 30 to‘ 300 parts of grape sugar
successively. Said liquid is then neutralized by
adding from 0.1 to 0.2 part of colloidsol of sodium
silicate. Then the same is admixed with the ac
tive element oi.’ suprarenal capsule, e. g. 5 parts
55 of‘ 1000 fold solution of adrenalin, and a small
cow upon the human body. But the use 01' this 35
kind of vaccine has been abolished since 1864,
for fear of spreading such infectious diseases as
syphilis, tuberculosis, skin diseases.
(2) Original vaccine
40
This is a matter taken from the vesicle pro- '
duced naturally on the cow's teat. But it is in
convenient inasmuch as this kind of vaccine is
not always available.
45
(3) Revaccinated vaccine
This is a matter taken from the vesicle produced
by revaccinating humanized vaccine upon the
body of a calf.
-
"
(4) Animal vaccine
50
This is a matter taken from the vesicle pro
duced by inoculating the original cow lymph upon
the body of another calf.
-
-
,
Among the above-mentioned, (B), (2) and (4) 55
2,119,507
2
then adding the active elements of a bird's
are both improvements upon Jenner's method to.
I
ovum
consisting of embryonic ectoblast tissue
and it is the revaccinated vaccine that isused, and plasma of the urethral‘ membrane obtained
in every country at present. ,
during the process of its hatching, thereby ob
But all these cow'lymphs have severe reaction taining a cultural medium, and then transplant
when they are inoculated upon the human body. ing and cultivating the virus of smallpox in said
'In order to avoid such a defect, sometimes the
2. A process for the production of vaccine
rabbit is used as material. _
And although in common use, vaccines which lymph comprising adding peptone, salt and sugar
are produced upon the body of the calf are min
to meat iuice, neutralizing the product with col
gled with various kinds oi! germs and contain such
10 animal organizations as the spore‘, lymph or scab loidsol of sodium silicate, adding adrenalin there
to, then adding‘the active elements of, a bird's
of the calf, causing severe secondary reactions ovum consisting of embryonic ectoblast tissue
,when inoculated upon the human body.
and plasma of‘ the urethral membrane obtained
As for the miscellaneous bacteria mixed in the during the process of its hatching, thereby ob
virus of smallpox, it has been proposedhereto
medium, and then transplant15 fore to sterilize them, mixing 1 part of vaccine taining a‘ cultural the virus of smallpox in said
ing and cultivating
lymph with 3 parts'of 80% water ‘solution of cultural medium.
cultural medium.
glycerlne, and storing. it in a refrigerator for a
period of one or two months. It has been also
to add from 0.6 to 0.8% of carbolic acid
to the glycerinated virus for the, same purpose.
But I have found that all the above-mentioned
existing methods were unable to make the virus
perfectly nonbacilli.
V
‘ Contrary to any of the old processes which rely
upon vital body such as animal body in cultivat
ing the virus, according to this invention the virus
‘ - '
‘
‘
10
.
15
.
3. A process'for the production. or vaccine
lymph comprising adding‘ peptone, salt and sugar 20
to meat juice, neutralizing the product with 'col
loidsol of alkali carbonate, adding adrenalin
thereto, then adding the active-elements of a
bird's ovum consisting of embryonic ectobla'st
tissue and plasma of the urethral membrane ob 25
tained during the process of its hatching, thereby '
obtaining a cultural medium, and then trans
planting and cultivating ‘the virus of small pox
of smallpox is transplanted and cultivated upon I in said cultural medium.
the arti?cial nutriment media, so that it is pos
4. A process for the production of vaccine
sible to easily and economically produce. the virus
pure from any othergerms, which can make the
human body immune with very slight reaction on
lymph comprising adding aminoacid, salt and
sugar to meat juice, neutralizing the product with ‘
colloidsol of sodium silicate, adding adrenalin
then ad ng the active elements of a . '
The vaccine, prepared according to the present thereto,
bird's ovum co isting of embryonic ectoblast 35
tissue and plasma of the urethral membrane ob
35 invention, has been successfully subjected to a
large number of experimental tests, using both tained during the process of its hatching, there
animal and human subjects, and thus verifying by obtaining a cultural medium, and then trans
the development of the virus of smallpox in the planting and cultivating the virus of smallpox in
culture media of the invention, a positive resultv said cultural medium.
49
having been obtained in every case. In making
5. A process ‘for the production of vaccine
these tests, hypodermic inoculation was employed.‘ lymph comprising adding aminoacid, salt and
However, the vaccine of the present invention sugar to meat juice, neutralizing the product
‘may also be used with the cutting method or by with colloidsol of alkali carbonate, adding adren
injection. The utility of the invention is evident alin thereto, then adding the active elements of 45
a bird's cvum'consisting of embryonic ectoblast
'
45 from the foregoing.
tissue and plasma of the urethral membrane ob
What I claim is:- ‘
'
v
1. A process for the production of vaccine tained during the process of its hatching, there
lymph comprising adding an aminoacld-contain
by obtaining a cultural medium, and then trans
ing substance, salt and sugar to meat juice, neu
planting and cultivating the virus of smallpox in _
tralizing the product with a member of the group said cultural medium._
50
consisting of the colloidsols of sodium .silicate
1
‘KISHIRO SUKEGAWA.
_ the skin after the’inoculation. '
and sodium carbonate-adding adrenalin there
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