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Патент USA US2125508

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2,125,508
Patented Aug. 2, 1938
; UNITED, STATES2,125,508'VP'ATENT, OFFICE
PROCESS OF PREPARING A SUBSTANCE‘
‘PROMOTING LACTATION
Carl Ludwig Lautenschliiger, Frankfort-on-the
Main, and Willy Ludwig, Frankfort-on-the
Main-Hochst, Germany, assignors ‘to Winthrop
.Chemical Company, Inc., New York, N. Y., a
corporation of New York .
.
No Drawing. Application January 9. 1937, Serial
No. 119,868. In Germany January ‘15, 1936
4 Claims.
(01. rev-L74)
The present invention relates to the’ prepara
tion of ‘the substance promoting lactation from
the anterior lobes of the hypophysis.
The parent material for the preparation of
5 the substance promoting lactation has been
fresh anterior lobes of the hypophysis or dried
glands which contain all the active substances
of the gland. Many attempts have been made
to isolate and to separate these substances from
10 the extracts containing them. These attempts
have always been accompanied by very consider
able losses; the separation of the gonad-stimu
mental separation of these two constituents from
‘
lating substance from that promoting lactation
is especially di?icult.
15
,
Now we have found that the substance pro
moting lactation may be prepared by extracting
the gonad-stimulating substance from the an
terior lobes of the hypophysis and extracting the
‘substance promoting lactation from the thus pre
2 O ‘ treated gland.
As starting material there may,
for instance, be used glands which have been
prev-treated by the process described in U. S.
application Serial No. 94,877, filed August '7, 1936,
in the name of Willy Ludwig. According to the
25 process described in that patent application the
a common solution.
The following examples serve to illustrate the,
invention, but they are not intended to limit it
thereto:
'
_
5
1. The moist residue of 15 grams of the an
terior lobes of the hypophysis which previously
have been extracted in the cold state four times
with 15 grams of vacetone each and then once
with water is stirred with 150 cc. of water and 10
the mixture is adjusted with N-caustic soda
solution to a pH-value of 8.5. The mixture is
then extracted and centrifuged for one hour at
35° C. This mixture is then extracted twice in
the same manner at a pH-value of 9.0 and 10.0 15
respectively.
The united extracts are again centrifuged, the
clear solution is adjusted to a pH-value of 5.0,
and the precipitate thus produced is dried with
alcohol. -
20 ~
2. The moist residue of 15 grams of the an—
terior lobes of the hypophysis which previously
have been extracted in the cold state four times
with 15 grams of acetone each and then once
with water is extracted and centrifuged (as de- 25
gonad-stimulating substance and other active . scribed ‘in Example 1) at room temperature and
substances of the hypophysis are extracted and
obtained in a high yield.
During the said process'the anterior lobes oi
30 the hypophysis are extracted at a temperature
not exceeding 60° C. with the aid of an organic
solvent miscible with water and then with
aqueous solvents. If the glands thus pre-treated
and freed from the gonad-stimulating substance
35 are treated with a strong alkali within the pH
limits of about 8.0 to about 12.0 in a manner
similar to that in which non-pretreated hyp0ph~
ysis has hitherto' been treated, there is ob
tained an extract of the substance promoting
40 lactation which is to, a large extent free from the
gonad-stimulating substance. This extract is
further treated, for instance, by precipitating the
at a pH-value of 8.5 to 11.5. The residue ob
tained by the subsequent precipitation at a
pH-value vof 5.0 is redissolved at a pH-value of
8.0 with addition of a disinfecting'agent, for in- 30
stance, sodium 2-ethyl-mercury-mercapto-ben
zoxazoie-S-carboxylate the solution is brought to
a proportion of 1:10000 and dried in a frozen
state under reduced pressure.
We claim: '
‘
‘
35
1. Process of preparing a substance promot
ing lactation which comprises extracting the an
terior lobes of the hypophysis at a temperature
not exceeding 60° C. with the aid of organic
solvents miscible with water, extracting the re- 40
maining gland residue with water and then with
an aqueous alkaline solution of a pH-value of
about 8'to.about 12 and precipitating the active
active substance near the isoelectric point i. e.
substance from the alkaline extract by acidi?ca
at a pH-value of about 5.5,‘ redissolving it in . tion.
g
'
feebly alkaline water, suitably with addition of
a disinfecting agent, freezing and drying the
solution.
.
_
The improved industrial advantage of the in
50 vention resides'in the fact that from the same
gland material there may be obtained, in a yield
of nearly 80 to 90 per cent, the important con
stituents, that are the gonad-stimulating sub-1
stance and the substance promoting lactation,
55 without the necessity for a subsequent detri
45
2. Process of preparing a substance promoting
_ ~lactation which comprises extracting the an
terior lobes of the hypophysis at a temperature
not exceeding 60° C. with the aid of organic
solvents miscible with water, extracting the 50
remaining gland residue withlwater and then
with an aqueous alkaline solution of a pH-value
of about 8 to about 12 and precipitating the
active substance from they alkaline extract by
acidifying it to a pH~value of 5.5.
-
55
2
2,125,508
i 3. Process of preparing a substance prcmoting ' terior lobes of the hypophysisat a temperature
iactation: which comprises extracting the an
not exceeding 60° C. with the aid of organic sol
terior lobes of the hypophysis at a temperature
not exceeding 60° C. with thegaid of organic
solvents miscible with water, extracting the re
maining gland residue with water and then with
an aqueous alkaii solution of a pH-value of about
8 to about 12 and precipitating the active sub
stance from the alkaline extract by acidifying
10 it to a pH-value of 5.5, redissolving the‘ precipi
Itate in an aqueous alkaline solution, freezing and
drying the solution.
1*
4. Process of preparing a substance promoting
lactation which comprises extracting the an
vents miscible with water, extracting the re
maining gland residueriwithv water andrthen with
an aqueous alkali Asolution of a pH-value of
about 8 to abe-ut 12 and precipitating the active
substance from the alkaline extract by acidifying
it to a.pH-va.lue of- 5.5, redissolving the precipi
tate in an aqueous alkaline solution in the pres
ence of a disinfecting agent, freezing and dry- 10
mg the solution.
CARL LUDWiG LAi?'E'NscHLAGEa."
WILLY LUDWIG.'_
'
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