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How to submit a Mass Spectrometry Sample:

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How to submit a Mass Spectrometry Sample:
When submitting a sample for analysis make sure that your requisition sheet is filled out completely and carefully. If you are unsure of which ionization technique (EI/CI/ESI) is required, ask one of the lab personnel. Dr.I. Starke phone: 5150 or S.Fürstenberg phone: 5294, 5293
In general, if your sample decomposes while obtaining a melting point, then you will not be able to request a electron impact ionization spectrum (EI). Electrospray ionization (ESI) will be required. Usually till 1.5 miligram of your sample are sufficient for an analysis.
Ionization Techniques available
Electron impact ionization (EI)
is the classical ionization technique in mass spectrometry; however, its application is restricted to thermally stable samples with low molecular mass (m/z 650). An EI spectrum contains many signals of fragment-ions from the compound and is therefore useful for structural characterization and identification of a compound. Electrospray ionization (ESI)
is the method of choice for proteins, oligosaccharides, oligonucleotides and complexes. However, the sample must be soluble and stable in protic solvents. For an overview of the instruments available, see our :
Instruments
1. The GC-MS systems SSQ710 and TRACE DSQII are single quadrupole mass spectrometer equipped with an Electron impact ionization source. Mass range: up to 1050 Da. Direct inlet port and GC ( Low resolution spectra) Concentration of the samples : 1 mg / 100 microliter
2. The GC-TOF is a Time of flight mass spectrometer equipped with an Electron impact (EI) source. Mass range : up to 1.500 Da, Direct inlet port and GC. Concentration of the samples : about 1 mg
3. The Q-TOF is a Time of flight mass spectrometer equipped with an Electrospray ionization (ESI) source. Mass range : up to 20 kDa Concentration of the samples : 0.1 - 1.5 mg (exact sample amount is needed)
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