close

Вход

Забыли?

вход по аккаунту

?

Virus Replication

код для вставкиСкачать
Virus Replication
Hsiao-Sheng Liu
еЉ‰ж Ўз”џ
ењ‹з«‹ж€ђеЉџе¤§е­ёеѕ®з”џз‰©жљЁе…Ќз–«е­ёз ”з©¶ж‰Ђ
The objectives:
• How to calculate virus titer?
• One step growth curve
• The general stages of viral replication
cycle
• Attachment, penetration and release of
the viruses
How to measure virus titer?
Baculovirus plaques
Zones of clearing (plaques) are generated by infection of insect (Sf9} cells
with individual baculovirus particles. Uninfected Sf9 cells surrounding the
plaque are stained pink with neutral red.
10-6
10-7
PFU: plaque forming unit
211 plaques
1 PFU = 1 plaque = 1 bacterial phage
Count : plaque 數量 x 稀釋倍數 x 10/9 = PFU/ml
Ex: 211 x 107 x 10/9 = 2.34 x 109 PFU/ml
What is one step growth curve?
й™°и”Ѕжњџ
жЅ›дјЏжњџ
What are the general stages in
viral replication cycles?
General replication
procedure
Downloaded from: StudentConsult (on 15 May 2010 10:15 AM)
В© 2005 Elsevier
Complicated replication
procedure (e.g. Herpes virus)
How the viral replication cycle is
known?
Attachment: receptor, tissue
tropism
HA: Hemagglutinine
Trimmers of HA on
influenza virus membrane
Coreceptor: CCR5
CRCX4
CCR5: macrophage-tropic HIV
CXCR4: lymphocyte-tropic HIV
How does the virus enter
the cell?
Fusion
Endocytosis
Pinocytosis (Viropexis)
Paramyxovirus fusion пѓ Syncytia
Togavirus
How is the virus released from
the cell?
Orthomyxovirus
with M protein
Herpes virus
Respiratory syncytial virus budding
Brooks et al, 1998
Документ
Категория
Презентации
Просмотров
11
Размер файла
13 752 Кб
Теги
1/--страниц
Пожаловаться на содержимое документа