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Microscope Review

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Microscope Review
Give the name and function
of each structure
labeled.
A – Ocular: eyepiece used to
look at specimen
B – Fine adjustment: to focus
specimen under high power
C – Arm: used to hold
microscope
D – Objective lens: used to
magnify image
E – Coarse adjustment: Used to
focus specimen under low
power
F – Diaphragm – adjust amount
of light reaching specimen
E
F
What was the highest
possible
magnification that
can be obtained
when using this
microscope?
Objective - Ocular
40 x 10 =
400x
E
F
What happens to the
amount of light in the
field of view when
switching from low to
high power?
E
The amount of light
decreases (so the
field of view gets
darker)
F
What happens to the
field of view when
switching from low to
high power?
E
The field of view
decreases ( so you
see less of the
slide).
F
Which structure can only
be used to focus the
specimen under high
power? Support your
answer.
The fine adjustment
because using the
coarse adjustment
under high power can
break the objective
lens or the slide.
E
F
What are two possible adjustments that
need to be made when switching to
high power?
Adjust the diaphragm to make the filed
of view brighter.
Focus with the fine adjustment.
What happens to the number of cells
that are visible in the field of view
when switching to high power?
The number of cells that are visible in
the field of view decreases.
What must be done to the
microscope before switching from
low power to high power?
Center the specimen
Focus with the coarse adjustment
Which laboratory technique is
illustrated in the diagram?
Preparing a wet mount.
Describe the steps to follow when
preparing a wet mount.
1. Place a drop of water on slide.
2. Place specimen on slide.
3. Place the cover slip at an angle.
4. Slowly lower the cover slip on to the
specimen.
Why must the cover slip be placed
at an angle when preparing a wet
mount?
To avoid air bubbles.
The diagram represents a cell in the field of
view of a compound light microscope. In
which direction should the slide be moved
on the microscope stage to center the cell
in the field of view?
Towards C
The image to the left is placed on the
slide. A student then views the
letter “f” under low power. Which
diagram below most closely
resembles the image under low
power?
A student sees the image to the left
when observing the letter "f" with
the low-power objective lens of a
microscope. Which diagram below
most closely resembles the image
the student will see after
switching to high power?
The total magnification of an image is
the result of the combined
magnifications of the
1) eyepiece and diaphragm
2) objective and eyepiece
3) objective and mirror
4) low-power and high-power
objectives
A student views some cheek cells
under low power. Before switching
to high power, the student should
(1) adjust the eyepiece
(2) center the image being viewed
(3) remove the slide from the stage
(4) remove the coverslip
A student changes the objective of a
microscope from 10x to 50x. If
this is the only change made, what
will happen to the field of view?
(1) Its diameter will decrease.
(2) Its diameter will increase.
(3) Its brightness will increase.
(4) Its brightness will remain the
same.
A student determined that exactly 10
pollen grains could fit along the
diameter of the field of view of a
microscope. If each pollen grain has a
diameter of 200 micrometers, what is
the diameter in millimeters of this
microscope's field of view?
(1) 2,000 mm
(2) 2 mm
(3) 5 mm
(4) 500 mm
A student is using a compound light
microscope to observe a wet mount of
unstained human cheek cells. Which
cell organelle will most likely become
more visible after she adds iodine
solution to the slide?
(1) nucleus
(2) ribosome
(3) cell wall
(4) mitochondrion
To locate a specimen on a prepared slide with a
compound microscope, a student should begin with
the low-power objective rather than the highpower objective because the
(1) field of vision is smaller under low power than
under high power
(2) field of vision is larger under low power than
under high power
(3) specimen does not need to be stained for
observation under low power but must be stained
for observation under high power
(4) amount of the specimen that can be observed
under low power is less than the amount that can be
observed under high power
An unstained wet mount preparation
of cheek cells shows few details.
Which substance could be added
to the slides to make the details
more visible?
(1) methylene blue
(2) dilute acid
(3) Benedict's solution
(4) bromthymol blue
After switching from the high-power to
the low-power objective lens of a
compound light microscope, the area
of the low-power field will appear
(1) larger and brighter
(2) smaller and brighter
(3) larger and darker
(4) smaller and darker
The diagram represents a hydra as viewed with
a compound light microscope. If the hydra
moves to the right of the slide preparation,
which diagram below best represents what
will be viewed through the microscope?
A cover slip should be used for
preparing a
(1) frog for dissection
(2) solution of iodine for food testing
(3) wet mount of elodea (a simple plant)
(4) test to determine the pH of a
solution
Which part of a microscope should be
used with the low power objective,
but not with the high-power
objective?
a. fine adjustment
b. diaphragm
c. coarse adjustment
d. eyepiece
A prepared slide was placed on the stage of a
microscope so that the slide faced the
student, as shown in the diagram below.
Which image will the student most likely see?
Convert:
1. 6.23mm
2. 5678um
3. .093mm
4. 48.2um
6230
= ______
um
5.678
= ______
mm
93
= ______
um
.0482
= ______
mm
The diagram represents the field of view of a
compound light microscope. Three
unicellular organisms are located across the
diameter of the field. What is the
approximate length of each unicellular
organism?
1) 250 um
2) 500 um 3) 1,000 um 4) 1,500 um
This organism could be centered in the field
of view by moving the microscope slide
towards
(1) A
(2) B
(3) C
(4) D
250um
500um
125um
The diameter is 1mm. What is the length
of the cell in um? 375um
1750um
3.5mm
3500um
The length of the cell is approximately 1750um.
There are 3 cells
across the field
of view.
1500/3 = 500um
Field of view = 1.5mm
= 1500um
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