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Lecture 11b: CDC/NIH Vertebrate Animal Biosafety Level Criteria

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CDC/NIH Vertebrate Animal
Biosafety Level Criteria
в–є For
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all Animal Biosafety Levels (1 - 4)
IACUC Approval
Authorized access to facilities
Animal handling training
Medical surveillance program
Written safety manual(s)
Pest control program
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є For
all Animal Biosafety Levels (1 - 4)
no eating, drinking, smoking, touching face
no food or drink storage within facilities
minimize aerosols
decontaminate work surfaces, equipment
transport wastes in leak-proof covered
containers
 handle sharps safely

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CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є For
all Animal Biosafety Levels (1 - 4)
 Protective clothing recommended, not worn
outside facility
 Facilities separate from general building traffic
 External doors self-closing/self-locking
 Animal room doors open inward, self-closing
 Water resistant, easily cleaned surfaces
(horizontal surfaces kept to a minimum)
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є For
all Animal Biosafety Levels (1 - 4)
 Sealed, break resistant windows
 Floor drain traps filled with water or
disinfectant
 No recirculation of exhaust air
 Rooms have negative pressure gradient to
adjacent hallway
 Hand washing sink available in facility
 180 F cage wash rinse temperature
 Adequate illumination
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є
Animal Biosafety Level 2 (ABSL2)
 Restrict access to few as possible (advise support
staff of potential hazards)
 Biohazard sign posted on animal room entrance
(hazard ID, contact info., & entry requirements)
 Immunizations, serum surveillance
 Labeled leakproof carriers, exterior disinfected
before transport (wastes, tissues, etc.)
 Safe sharps policies (plasticware, safe sharps
devices, limited use of needles/syringes)
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 2 (ABSL2)
 Limited to animals dedicated to work
 Exposures, spills reported immediately
 Gloves, gowns, uniforms or lab coats worn
in rooms (removed prior to exit)
 Face protection (goggles, safety glasses,
full face shields, masks) selected on basis
of risk
 Biosafety cabinets used to confine aerosol
procedures
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 2 (ABSL2)
 Filter top cages, cage dumping
containment stations used where
appropriate
 Autoclave available
 hand washing sink in animal room
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 3 (ABSL3)
 Individuals at increased risk of infection
not allowed in facility
 All wastes treated before disposal as
regulated biomedical waste (incineration
recommended)
 Specialized training provided and
updated
 Cages autoclaved/decontaminated
before bedding is removed
 Spill procedures posted
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 3 (ABSL3)
 Uniforms or scrub suits covered by
solid-front gown
 Face/eye, and where necessary,
respiratory protection worn by all who
enter animal rooms
 Boots, shoe covers, etc. worn when
needed
 Consider use of containment ventilated
cage units, housing in biosafety
cabinets, of filter bonnets.
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 3 (ABSL3)
 All procedures performed in biosafety
cabinet (wear respiratory protection if
working outside cabinet)
 Consider clothes change room and
shower
 Penetrations in rooms are sealed or
capable of being sealed
 Hands-free sink near exit door
 Sink-trap filled with disinfectant after
each use
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 3 (ABSL3)
Ducted exhaust ventilation system
Exhaust discharge away from air intakes,
Consider HEPA filtration of exhaust air
Room airflow monitoring device (personnel
verify airflow direction upon entry)
 Consider alarms for ventilation system failure
 Consider interlock of exhaust/supply systems
to avoid pressurization
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CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 3 (ABSL3)
 Biosafety cabinets tested/certified
annually
 Autoclave located near BL3 suite
 Vacuum system protected with HEPA
filter if used
 Initial and annual verification of BL3
facility design parameters
 Consider additional facility
enhancements as needed (effluent
decontamination, showers, etc)
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 4 (ABSL4)
 Program includes post-exposure
counseling and prophylaxis
 Site-specific biosafety manual prepared
 Double-door pass through autoclave
 Procedures performed in pairs
 Materials unrelated to work not allowed
in facility
 Additional access control measures
(security guard)
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 4 (ABSL4)
 Complete clothes change, personal shower
upon exit
 All material in/out of facility through pass
through autoclave or disinfectant chamber
 Autoclave door interlocked to prevent both
open simultaneously
 Chart exposures, accidents/incidents,
employee absenteeism, appearance of
related signs/symptoms of infection
CDC/NIH Verterbrate Animal
Biosafety Level Criteria
в–є Animal
Biosafety Level 4 (ABSL4)
 Serum samples analyzed at defined
intervals
 Positive pressure suits or Class III
biosafety cabinet line
 Very unique engineering requirements
for BL4 laboratories (dedicated building
within building concept)
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